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The role of bloom index of gelatin on the interaction with retinal pigment epithelial cells.

Lai JY - Int J Mol Sci (2009)

Bottom Line: This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells.The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups.These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan. jylai@mail.cgu.edu.tw <jylai@mail.cgu.edu.tw>

ABSTRACT
Biocompatible materials are of considerable interest in the development of cell/drug delivery carriers for therapeutic applications. This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells. Following two days of culture of ARPE-19 cells with gelatin samples G75-100, G175, and G300, the in vitro biocompatibility was determined by cell proliferation and viability assays, and glutamate uptake measurements, as well as cytokine expression analyses. The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups. The Live/Dead assays also showed that the gelatin samples G300 induced mild cytotoxicity. In comparison with the treatment of gelatins with low Bloom index, the exposure to high Bloom strength gelatins markedly reduced the glutamate uptake capacity of ARPE-19 cells. One possible explanation for these observations is that the presence of gelatin samples G300 with high viscosity in the medium may affect the nutrient availability to cultured cells. The analyses of pro-inflammatory cytokine IL-6 expression at both mRNA and protein levels showed that the gelatins with low Bloom index caused less cellular inflammatory reaction and had more acceptable biocompatibility than their high Bloom strength counterparts. These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

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Mean percentage of live cells in the ARPE-19 cultures exposed to various dissolved gelatin materials as measured by the Live/Dead assay.An asterisk indicates statistically significant differences (*p<0.05; n = 6) as compared to controls (without materials).
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f4-ijms-10-03442: Mean percentage of live cells in the ARPE-19 cultures exposed to various dissolved gelatin materials as measured by the Live/Dead assay.An asterisk indicates statistically significant differences (*p<0.05; n = 6) as compared to controls (without materials).

Mentions: Figure 3 is a representative photograph of ARPE-19 cells labeled with Live/Dead stain, where the live cells fluoresce green and the dead cells fluoresce red. In the control groups, the majority of confluent cell cultures are viable (Figure 3A and B). After exposure to gelatin samples G75-100 for two days at 37 °C, the ARPE-19 cultures maintain good viability with only a few dead cells (Figure 3C and D). By contrast, in the G175 and G300 groups, an increased number of red-stained nuclei were noted, indicating that these gelatin samples induced mild cytotoxicity (Figure 3E-H). Figure 4 shows the mean percentage of live cells as determined by the Live/Dead assay. The cell viability was significantly lower in the G300 groups (89.4 ± 1.9%), compared with those of the control (99.2 ± 0.6%), G75-100 (98.5 ± 1.2%), and G175 groups (96.7 ± 1.7%; p<0.05). These findings suggest that the gelatins with low Bloom index are more cytocompatible than their high Bloom strength counterparts.


The role of bloom index of gelatin on the interaction with retinal pigment epithelial cells.

Lai JY - Int J Mol Sci (2009)

Mean percentage of live cells in the ARPE-19 cultures exposed to various dissolved gelatin materials as measured by the Live/Dead assay.An asterisk indicates statistically significant differences (*p<0.05; n = 6) as compared to controls (without materials).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2812822&req=5

f4-ijms-10-03442: Mean percentage of live cells in the ARPE-19 cultures exposed to various dissolved gelatin materials as measured by the Live/Dead assay.An asterisk indicates statistically significant differences (*p<0.05; n = 6) as compared to controls (without materials).
Mentions: Figure 3 is a representative photograph of ARPE-19 cells labeled with Live/Dead stain, where the live cells fluoresce green and the dead cells fluoresce red. In the control groups, the majority of confluent cell cultures are viable (Figure 3A and B). After exposure to gelatin samples G75-100 for two days at 37 °C, the ARPE-19 cultures maintain good viability with only a few dead cells (Figure 3C and D). By contrast, in the G175 and G300 groups, an increased number of red-stained nuclei were noted, indicating that these gelatin samples induced mild cytotoxicity (Figure 3E-H). Figure 4 shows the mean percentage of live cells as determined by the Live/Dead assay. The cell viability was significantly lower in the G300 groups (89.4 ± 1.9%), compared with those of the control (99.2 ± 0.6%), G75-100 (98.5 ± 1.2%), and G175 groups (96.7 ± 1.7%; p<0.05). These findings suggest that the gelatins with low Bloom index are more cytocompatible than their high Bloom strength counterparts.

Bottom Line: This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells.The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups.These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan. jylai@mail.cgu.edu.tw <jylai@mail.cgu.edu.tw>

ABSTRACT
Biocompatible materials are of considerable interest in the development of cell/drug delivery carriers for therapeutic applications. This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells. Following two days of culture of ARPE-19 cells with gelatin samples G75-100, G175, and G300, the in vitro biocompatibility was determined by cell proliferation and viability assays, and glutamate uptake measurements, as well as cytokine expression analyses. The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups. The Live/Dead assays also showed that the gelatin samples G300 induced mild cytotoxicity. In comparison with the treatment of gelatins with low Bloom index, the exposure to high Bloom strength gelatins markedly reduced the glutamate uptake capacity of ARPE-19 cells. One possible explanation for these observations is that the presence of gelatin samples G300 with high viscosity in the medium may affect the nutrient availability to cultured cells. The analyses of pro-inflammatory cytokine IL-6 expression at both mRNA and protein levels showed that the gelatins with low Bloom index caused less cellular inflammatory reaction and had more acceptable biocompatibility than their high Bloom strength counterparts. These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

Show MeSH
Related in: MedlinePlus