Limits...
Involvement of nitric oxide in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.

Veeresh B, Patil BM, Veeresh Babu SV, Jeedi NM, Unger BS - Indian J Pharmacol (2009)

Bottom Line: Intravenous administration of 1-phenylbiguanide (1-PBG) induced a net secretion of fluid in both jejunum and colon. 1-PBG had a more prominent secretory effect in the colon, causing a three-fold increase in volume of fluid secreted/g of colon than in the jejunum.Pretreatment with (L-NNA) prevented the 1-PBG-induced fluid accumulation in both jejunum and colon.The inhibitory effect of L-NNA on 1-PBG-induced fluid accumulation was reversed by L-Arg but not by D-Arg.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, K.L.E.Ss College of Pharmacy, J.N.M.C. Campus, Nehru Nagar, Belgaum - 590 010, India.

ABSTRACT

Objectives: The aim of the present study was to investigate the involvement of nitric oxide in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.

Materials and methods: Fluid movement in jejunum and colon were determined simultaneously in the same rat, by modifying the Beubler method. Nomega-nitro-L-arginine (L-NNA, 20 mg/kg, s.c) alone and in combination with L-arginine (L-Arg, 150 mg/kg s.c) or D-arginine (D-Arg, 150 mg/kg, s.c) were administered 30 min before administration of 1-PBG (18.5 mug/kg, i.v).

Results: Intravenous administration of 1-phenylbiguanide (1-PBG) induced a net secretion of fluid in both jejunum and colon. 1-PBG had a more prominent secretory effect in the colon, causing a three-fold increase in volume of fluid secreted/g of colon than in the jejunum. Pretreatment with (L-NNA) prevented the 1-PBG-induced fluid accumulation in both jejunum and colon. The inhibitory effect of L-NNA on 1-PBG-induced fluid accumulation was reversed by L-Arg but not by D-Arg.

Conclusion: These results provide evidence that nitric oxide plays an important role in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.

No MeSH data available.


Effect of combined administration of L-NNA and L-arginine or D-arginine on 1-PBG-induced intraluminal fluid transport. Results are expressed as mean ± S.E.M. (n = 6). A negative value represents net absorption and positive value represents net secretion. ***P < 0.01, **P < 0.001 vs. 1-PBG; *P < 0.01 vs. 1-PBG + L-NNA
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2812781&req=5

Figure 0002: Effect of combined administration of L-NNA and L-arginine or D-arginine on 1-PBG-induced intraluminal fluid transport. Results are expressed as mean ± S.E.M. (n = 6). A negative value represents net absorption and positive value represents net secretion. ***P < 0.01, **P < 0.001 vs. 1-PBG; *P < 0.01 vs. 1-PBG + L-NNA

Mentions: L-arginine (150-600 mg/kg) reversed the effect of L-NNA (20 mg/kg) on 1-PBG-induced fluid accumulation in the gut in a dose-related fashion. A 600 mg/kg dose of L-arginine and a 300 mg/kg dose of L-arginine abolished completely the inhibitory effect of L-NNA in jejunum and colon, respectively. In contrast, D-arginine (300 mg/kg) did not alter the effects of L-NNA in rats treated with 1-PBG [Figure 2].


Involvement of nitric oxide in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.

Veeresh B, Patil BM, Veeresh Babu SV, Jeedi NM, Unger BS - Indian J Pharmacol (2009)

Effect of combined administration of L-NNA and L-arginine or D-arginine on 1-PBG-induced intraluminal fluid transport. Results are expressed as mean ± S.E.M. (n = 6). A negative value represents net absorption and positive value represents net secretion. ***P < 0.01, **P < 0.001 vs. 1-PBG; *P < 0.01 vs. 1-PBG + L-NNA
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2812781&req=5

Figure 0002: Effect of combined administration of L-NNA and L-arginine or D-arginine on 1-PBG-induced intraluminal fluid transport. Results are expressed as mean ± S.E.M. (n = 6). A negative value represents net absorption and positive value represents net secretion. ***P < 0.01, **P < 0.001 vs. 1-PBG; *P < 0.01 vs. 1-PBG + L-NNA
Mentions: L-arginine (150-600 mg/kg) reversed the effect of L-NNA (20 mg/kg) on 1-PBG-induced fluid accumulation in the gut in a dose-related fashion. A 600 mg/kg dose of L-arginine and a 300 mg/kg dose of L-arginine abolished completely the inhibitory effect of L-NNA in jejunum and colon, respectively. In contrast, D-arginine (300 mg/kg) did not alter the effects of L-NNA in rats treated with 1-PBG [Figure 2].

Bottom Line: Intravenous administration of 1-phenylbiguanide (1-PBG) induced a net secretion of fluid in both jejunum and colon. 1-PBG had a more prominent secretory effect in the colon, causing a three-fold increase in volume of fluid secreted/g of colon than in the jejunum.Pretreatment with (L-NNA) prevented the 1-PBG-induced fluid accumulation in both jejunum and colon.The inhibitory effect of L-NNA on 1-PBG-induced fluid accumulation was reversed by L-Arg but not by D-Arg.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, K.L.E.Ss College of Pharmacy, J.N.M.C. Campus, Nehru Nagar, Belgaum - 590 010, India.

ABSTRACT

Objectives: The aim of the present study was to investigate the involvement of nitric oxide in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.

Materials and methods: Fluid movement in jejunum and colon were determined simultaneously in the same rat, by modifying the Beubler method. Nomega-nitro-L-arginine (L-NNA, 20 mg/kg, s.c) alone and in combination with L-arginine (L-Arg, 150 mg/kg s.c) or D-arginine (D-Arg, 150 mg/kg, s.c) were administered 30 min before administration of 1-PBG (18.5 mug/kg, i.v).

Results: Intravenous administration of 1-phenylbiguanide (1-PBG) induced a net secretion of fluid in both jejunum and colon. 1-PBG had a more prominent secretory effect in the colon, causing a three-fold increase in volume of fluid secreted/g of colon than in the jejunum. Pretreatment with (L-NNA) prevented the 1-PBG-induced fluid accumulation in both jejunum and colon. The inhibitory effect of L-NNA on 1-PBG-induced fluid accumulation was reversed by L-Arg but not by D-Arg.

Conclusion: These results provide evidence that nitric oxide plays an important role in 5-HT(3) receptor agonist-induced fluid accumulation in jejunum and colon of anesthetized rats.

No MeSH data available.