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Expression of TLR2, TLR4, and TLR9 in dermatomyositis and polymyositis.

Kim GT, Cho ML, Park YE, Yoo WH, Kim JH, Oh HJ, Kim DS, Baek SH, Lee SH, Lee JH, Kim HY, Kim SI - Clin. Rheumatol. (2009)

Bottom Line: The expressions of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were measured by real-time reverse transcription-polymerase chain reaction in muscle tissues from 14 patients with DM and PM (nine patients with DM, five patients with PM) and three controls.The expression levels of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were significantly high in patients with DM and PM compared with those in the controls, and the expression levels of TLR4 and TLR9 had significant positive correlations with the expressions of IFNgamma, IL4, IL17, and TNFalpha.These results suggest that the involvement of TLR4 and TLR9 in immunopathogenesis of DM and PM might be connected with activation of CD4(+) T cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Department of Internal Medicine, School of Medicine, Pusan National University, 1-10, Ami-Dong, Seo-Gu, Busan, South Korea.

ABSTRACT
The aim of this study was to investigate the expressions of Toll-like receptor (TLR) 2, TLR4, TLR9, and their correlations with the expression of cytokines that are associated with activation of CD4(+) T cells and inflammation including interferon gamma (IFNgamma), interleukin 4 (IL4), interleukin 17 (IL17), and tumor necrosis factor alpha (TNFalpha) in muscle tissues of patients with dermatomyositis (DM) and polymyositis (PM). The expressions of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were measured by real-time reverse transcription-polymerase chain reaction in muscle tissues from 14 patients with DM and PM (nine patients with DM, five patients with PM) and three controls. The expressions of TLR2, TLR4, and TLR9 were also localized with immunohistochemistry. The expression levels of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were significantly high in patients with DM and PM compared with those in the controls, and the expression levels of TLR4 and TLR9 had significant positive correlations with the expressions of IFNgamma, IL4, IL17, and TNFalpha. Immunohistochemistry showed that TLR2, TLR4, and TLR9 were expressed by infiltrating cells of perimysium in DM, whereas they were expressed by infiltrating cells of endomysium in PM. These results suggest that the involvement of TLR4 and TLR9 in immunopathogenesis of DM and PM might be connected with activation of CD4(+) T cells.

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Immunohistochemistry of TLR4 in muscle tissues of patients with DM, PM, and controls. In the muscle tissues of DM, TLR4 was expressed by infiltrating cells in the perimysial area (B3), whereas in PM, TLR4 was expressed by infiltrating cells in the endomysium (B4). Control muscle tissues were obtained from patients with non-specific muscle manifestations but with normal histological findings (B1, 2). Magnification was ×400
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Fig3: Immunohistochemistry of TLR4 in muscle tissues of patients with DM, PM, and controls. In the muscle tissues of DM, TLR4 was expressed by infiltrating cells in the perimysial area (B3), whereas in PM, TLR4 was expressed by infiltrating cells in the endomysium (B4). Control muscle tissues were obtained from patients with non-specific muscle manifestations but with normal histological findings (B1, 2). Magnification was ×400

Mentions: TLR2, TLR4, and TLR9 were expressed by perimysial infiltrating cells in DM, whereas they were expressed by endomysial infiltrating cells in PM (Figs. 2, 3, and 4).Fig. 2


Expression of TLR2, TLR4, and TLR9 in dermatomyositis and polymyositis.

Kim GT, Cho ML, Park YE, Yoo WH, Kim JH, Oh HJ, Kim DS, Baek SH, Lee SH, Lee JH, Kim HY, Kim SI - Clin. Rheumatol. (2009)

Immunohistochemistry of TLR4 in muscle tissues of patients with DM, PM, and controls. In the muscle tissues of DM, TLR4 was expressed by infiltrating cells in the perimysial area (B3), whereas in PM, TLR4 was expressed by infiltrating cells in the endomysium (B4). Control muscle tissues were obtained from patients with non-specific muscle manifestations but with normal histological findings (B1, 2). Magnification was ×400
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Related In: Results  -  Collection

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Fig3: Immunohistochemistry of TLR4 in muscle tissues of patients with DM, PM, and controls. In the muscle tissues of DM, TLR4 was expressed by infiltrating cells in the perimysial area (B3), whereas in PM, TLR4 was expressed by infiltrating cells in the endomysium (B4). Control muscle tissues were obtained from patients with non-specific muscle manifestations but with normal histological findings (B1, 2). Magnification was ×400
Mentions: TLR2, TLR4, and TLR9 were expressed by perimysial infiltrating cells in DM, whereas they were expressed by endomysial infiltrating cells in PM (Figs. 2, 3, and 4).Fig. 2

Bottom Line: The expressions of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were measured by real-time reverse transcription-polymerase chain reaction in muscle tissues from 14 patients with DM and PM (nine patients with DM, five patients with PM) and three controls.The expression levels of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were significantly high in patients with DM and PM compared with those in the controls, and the expression levels of TLR4 and TLR9 had significant positive correlations with the expressions of IFNgamma, IL4, IL17, and TNFalpha.These results suggest that the involvement of TLR4 and TLR9 in immunopathogenesis of DM and PM might be connected with activation of CD4(+) T cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Department of Internal Medicine, School of Medicine, Pusan National University, 1-10, Ami-Dong, Seo-Gu, Busan, South Korea.

ABSTRACT
The aim of this study was to investigate the expressions of Toll-like receptor (TLR) 2, TLR4, TLR9, and their correlations with the expression of cytokines that are associated with activation of CD4(+) T cells and inflammation including interferon gamma (IFNgamma), interleukin 4 (IL4), interleukin 17 (IL17), and tumor necrosis factor alpha (TNFalpha) in muscle tissues of patients with dermatomyositis (DM) and polymyositis (PM). The expressions of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were measured by real-time reverse transcription-polymerase chain reaction in muscle tissues from 14 patients with DM and PM (nine patients with DM, five patients with PM) and three controls. The expressions of TLR2, TLR4, and TLR9 were also localized with immunohistochemistry. The expression levels of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were significantly high in patients with DM and PM compared with those in the controls, and the expression levels of TLR4 and TLR9 had significant positive correlations with the expressions of IFNgamma, IL4, IL17, and TNFalpha. Immunohistochemistry showed that TLR2, TLR4, and TLR9 were expressed by infiltrating cells of perimysium in DM, whereas they were expressed by infiltrating cells of endomysium in PM. These results suggest that the involvement of TLR4 and TLR9 in immunopathogenesis of DM and PM might be connected with activation of CD4(+) T cells.

Show MeSH
Related in: MedlinePlus