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Cutaneous zygomycosis due to Saksenaea vasiformis in an immunocompetent host.

Baradkar VP, Kumar S - Indian J Dermatol (2009)

Bottom Line: Saksenaea vasiformis is an emerging zygomycete species, most often associated with cutaneous, subcutaneous and rhino- orbito-cerebral infections.Slide cultures were put up on Czapek Dox agar, which showed typical flask-shaped sporangium with rhizoids.The patient was treated successfully with intravenous amphotericin B.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Lokmanya Tilak Municipal Medical College & General Hospital, Sion, Mumbai - 400 002, India. vasantbaradkar@yahoo.com

ABSTRACT
Saksenaea vasiformis is an emerging zygomycete species, most often associated with cutaneous, subcutaneous and rhino- orbito-cerebral infections. Herein, we report a case of cutaneous zygomycosis of face caused by Saksenaea vasiformis in a 54-year-old immunocompetent female. The diagnosis was carried out by microscopy using KOH mount, Gram staining, Gomori's methenamine silver staining, hemotoxylin and eosin staining and culture on Sabouraud's Dextrose agar without actidione. Slide cultures were put up on Czapek Dox agar, which showed typical flask-shaped sporangium with rhizoids. The patient was treated successfully with intravenous amphotericin B.

No MeSH data available.


Related in: MedlinePlus

Ulcer on the face caused by Saksenaea vasiformis
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Figure 0001: Ulcer on the face caused by Saksenaea vasiformis

Mentions: A 54-year-old female presented with ulcerative wound over left cheek of approximately 6 × 6 cm in size for one month [Figure 1]. The patient gave a history of a small nodule three months back, which gradually increased in size before rupturing, following which, there was a whitish purulent discharge from the site. The patient did not give any history of any trauma to the affected part. The patient was nondiabetic, HIV seronegative and there was no history of any major illness in the past or any history suggestive of any immunocompromised state. Examination revealed that the patient was afebrile. The ulcer was slightly tender. Her blood investigations and liver and renal function tests were within normal limits. An emergency slough debridement was done and samples of excised skin and subcutaneous tissue were obtained for bacteriological and fungal culture and for histopathological examination. The material was processed for potassium hydroxide (KOH) preparation, Gram staining, Gomori's methenamine silver nitrate staining (GMS), hematoxylin and eosin staining. Gram stained smear showed broad aseptate hyphae, GMS staining showed brownish asepate hyphae [Figure 2], H and E stain also revealed aseptate hyphae. Bacterial cultures yield no growth of bacteria. There was creamish white cottony growth on Blood agar. There was no growth on Sabouraud's Dextrose (SDA) with actidione, however SDA without actidione kept at 22°C showed luxurious cottony growth after 48 h of incubation.


Cutaneous zygomycosis due to Saksenaea vasiformis in an immunocompetent host.

Baradkar VP, Kumar S - Indian J Dermatol (2009)

Ulcer on the face caused by Saksenaea vasiformis
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2807721&req=5

Figure 0001: Ulcer on the face caused by Saksenaea vasiformis
Mentions: A 54-year-old female presented with ulcerative wound over left cheek of approximately 6 × 6 cm in size for one month [Figure 1]. The patient gave a history of a small nodule three months back, which gradually increased in size before rupturing, following which, there was a whitish purulent discharge from the site. The patient did not give any history of any trauma to the affected part. The patient was nondiabetic, HIV seronegative and there was no history of any major illness in the past or any history suggestive of any immunocompromised state. Examination revealed that the patient was afebrile. The ulcer was slightly tender. Her blood investigations and liver and renal function tests were within normal limits. An emergency slough debridement was done and samples of excised skin and subcutaneous tissue were obtained for bacteriological and fungal culture and for histopathological examination. The material was processed for potassium hydroxide (KOH) preparation, Gram staining, Gomori's methenamine silver nitrate staining (GMS), hematoxylin and eosin staining. Gram stained smear showed broad aseptate hyphae, GMS staining showed brownish asepate hyphae [Figure 2], H and E stain also revealed aseptate hyphae. Bacterial cultures yield no growth of bacteria. There was creamish white cottony growth on Blood agar. There was no growth on Sabouraud's Dextrose (SDA) with actidione, however SDA without actidione kept at 22°C showed luxurious cottony growth after 48 h of incubation.

Bottom Line: Saksenaea vasiformis is an emerging zygomycete species, most often associated with cutaneous, subcutaneous and rhino- orbito-cerebral infections.Slide cultures were put up on Czapek Dox agar, which showed typical flask-shaped sporangium with rhizoids.The patient was treated successfully with intravenous amphotericin B.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Lokmanya Tilak Municipal Medical College & General Hospital, Sion, Mumbai - 400 002, India. vasantbaradkar@yahoo.com

ABSTRACT
Saksenaea vasiformis is an emerging zygomycete species, most often associated with cutaneous, subcutaneous and rhino- orbito-cerebral infections. Herein, we report a case of cutaneous zygomycosis of face caused by Saksenaea vasiformis in a 54-year-old immunocompetent female. The diagnosis was carried out by microscopy using KOH mount, Gram staining, Gomori's methenamine silver staining, hemotoxylin and eosin staining and culture on Sabouraud's Dextrose agar without actidione. Slide cultures were put up on Czapek Dox agar, which showed typical flask-shaped sporangium with rhizoids. The patient was treated successfully with intravenous amphotericin B.

No MeSH data available.


Related in: MedlinePlus