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Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags.

Maki N, Martinson J, Nishimura O, Tarui H, Meller J, Tsonis PA, Agata K - Mol. Vis. (2010)

Bottom Line: From the cDNA library 10,449 clones were sequenced and analyzed.From 10,449 reads, 780 contigs and 1,666 singlets were annotated.Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found.

View Article: PubMed Central - PubMed

Affiliation: Center for Developmental Biology, RIKEN Kobe, Kobe, Japan.

ABSTRACT

Purpose: The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level.

Methods: mRNA was purified from iris during dedifferentiation and its cDNA library was constructed. From the cDNA library 10,449 clones were sequenced and analyzed.

Results: From 10,449 reads, 780 contigs and 1,666 singlets were annotated. The presence of several cancer- and apoptosis-related genes during newt dedifferentiation was revealed. Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found.

Conclusions: The expression of cancer- and apoptosis-related genes could be hallmarks during dedifferentiation. The expression sequence tag (EST) resource is useful for the future study of newt dedifferentiation, and the sequence information is available in GenBank (accession numbers; FS290155-FS300559).

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Related in: MedlinePlus

Most frequently counted terms in the lens-regenerating iris. The fifteen most frequently counted terms of biologic process (A), cellular component (B), and molecular function (C) are shown. The total number of these most frequently counted terms was 5,356, 3,259, and 4,152, respectively. In the figure, ATP is adenosine triphosphate and GTP is guanosine triphosphate
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f1: Most frequently counted terms in the lens-regenerating iris. The fifteen most frequently counted terms of biologic process (A), cellular component (B), and molecular function (C) are shown. The total number of these most frequently counted terms was 5,356, 3,259, and 4,152, respectively. In the figure, ATP is adenosine triphosphate and GTP is guanosine triphosphate

Mentions: Functional annotation of the contigs and singlets was performed using the Blast2GO program. The 15 most frequently counted biologic process terms, cellular component terms, and molecular function terms are shown in Figure 1.


Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags.

Maki N, Martinson J, Nishimura O, Tarui H, Meller J, Tsonis PA, Agata K - Mol. Vis. (2010)

Most frequently counted terms in the lens-regenerating iris. The fifteen most frequently counted terms of biologic process (A), cellular component (B), and molecular function (C) are shown. The total number of these most frequently counted terms was 5,356, 3,259, and 4,152, respectively. In the figure, ATP is adenosine triphosphate and GTP is guanosine triphosphate
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2807617&req=5

f1: Most frequently counted terms in the lens-regenerating iris. The fifteen most frequently counted terms of biologic process (A), cellular component (B), and molecular function (C) are shown. The total number of these most frequently counted terms was 5,356, 3,259, and 4,152, respectively. In the figure, ATP is adenosine triphosphate and GTP is guanosine triphosphate
Mentions: Functional annotation of the contigs and singlets was performed using the Blast2GO program. The 15 most frequently counted biologic process terms, cellular component terms, and molecular function terms are shown in Figure 1.

Bottom Line: From the cDNA library 10,449 clones were sequenced and analyzed.From 10,449 reads, 780 contigs and 1,666 singlets were annotated.Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found.

View Article: PubMed Central - PubMed

Affiliation: Center for Developmental Biology, RIKEN Kobe, Kobe, Japan.

ABSTRACT

Purpose: The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level.

Methods: mRNA was purified from iris during dedifferentiation and its cDNA library was constructed. From the cDNA library 10,449 clones were sequenced and analyzed.

Results: From 10,449 reads, 780 contigs and 1,666 singlets were annotated. The presence of several cancer- and apoptosis-related genes during newt dedifferentiation was revealed. Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found.

Conclusions: The expression of cancer- and apoptosis-related genes could be hallmarks during dedifferentiation. The expression sequence tag (EST) resource is useful for the future study of newt dedifferentiation, and the sequence information is available in GenBank (accession numbers; FS290155-FS300559).

Show MeSH
Related in: MedlinePlus