Limits...
Distinct cytokine pattern in aqueous humor during immune reactions following penetrating keratoplasty.

Maier P, Heizmann U, Böhringer D, Kern Y, Reinhard T - Mol. Vis. (2010)

Bottom Line: We found statistically significant differences for IL-10 and INF-gamma among the study groups.We observed no significant differences concerning all other cytokines evaluated in this study.Perhaps this is a donor-specific activation of the immune privilege in the anterior chamber as an attempt to prevent damage during immune reactions.

View Article: PubMed Central - PubMed

Affiliation: University Eye Hospital and Lions Cornea Bank Baden-Württemberg, Freiburg, Germany. philip.maier@uniklinik-freiburg.de

ABSTRACT

Purpose: Cytokine patterns in the aqueous humor before penetrating keratoplasty might permit us to predict the development of immune reactions. We therefore analyzed ten different cytokines in the aqueous humor of patients before and following penetrating keratoplasty.

Methods: We collected aqueous humor from patients undergoing cataract extraction or penetrating keratoplasty (baseline, n=197), from patients undergoing cataract extraction after penetrating keratoplasty without signs of an endothelial immune reaction (acceptors, n=33), and from patients who underwent irrigation of the anterior chamber due to endothelial immune reactions (rejectors, n=25). We determined interleukin (IL)-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, interferon (INF)-gamma, and tumor necrosis factor-alpha in the aqueous humor, using cytometric bead arrays.

Results: We found statistically significant differences for IL-10 and INF-gamma among the study groups. We observed no significant differences concerning all other cytokines evaluated in this study.

Conclusions: Elevated levels of INF-gamma emphasize its important role in immune reactions following penetrating keratoplasty. Antagonizing INF-gamma might be an option when treating immune reactions. High levels of IL-10 during endothelial immune reactions may reflect a downregulation of the delayed-type hypersensitivity. Perhaps this is a donor-specific activation of the immune privilege in the anterior chamber as an attempt to prevent damage during immune reactions. We plan to investigate, in upcoming prospective trials, whether the analysis of cytokine patterns in the aqueous humor before penetrating keratoplasty will enable us to predict the occurrence of immune reactions.

Show MeSH

Related in: MedlinePlus

Random forest analysis demonstrates the relative importance of individual cytokines in classifying different study groups.Random forest analysis of data set 1 (A) and data set 2 (B) demonstrating the relative importance of individual cytokines in classifying aqueous humor from different study groups. The individual plots represent the relative importance of the cytokines in the overall classification. The vertical axes represent individual cytokines sorted by importance. The horizontal axes represent the average decrease in classification accuracy. Abbreviations: IL=interleukin, TNF=tumor necrosis factor, INF=interferon.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2807615&req=5

f1: Random forest analysis demonstrates the relative importance of individual cytokines in classifying different study groups.Random forest analysis of data set 1 (A) and data set 2 (B) demonstrating the relative importance of individual cytokines in classifying aqueous humor from different study groups. The individual plots represent the relative importance of the cytokines in the overall classification. The vertical axes represent individual cytokines sorted by importance. The horizontal axes represent the average decrease in classification accuracy. Abbreviations: IL=interleukin, TNF=tumor necrosis factor, INF=interferon.

Mentions: The random forest algorithm estimates the importance of a variable in discriminating among study groups. Therefore, we first performed this analysis on both data sets to identify which cytokines seemed key to permitting differentiation among study groups. The results of these analyses are demonstrated in Figure 1, which illustrates the relative importance of individual cytokines in classifying aqueous humor from different study groups.


Distinct cytokine pattern in aqueous humor during immune reactions following penetrating keratoplasty.

Maier P, Heizmann U, Böhringer D, Kern Y, Reinhard T - Mol. Vis. (2010)

Random forest analysis demonstrates the relative importance of individual cytokines in classifying different study groups.Random forest analysis of data set 1 (A) and data set 2 (B) demonstrating the relative importance of individual cytokines in classifying aqueous humor from different study groups. The individual plots represent the relative importance of the cytokines in the overall classification. The vertical axes represent individual cytokines sorted by importance. The horizontal axes represent the average decrease in classification accuracy. Abbreviations: IL=interleukin, TNF=tumor necrosis factor, INF=interferon.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2807615&req=5

f1: Random forest analysis demonstrates the relative importance of individual cytokines in classifying different study groups.Random forest analysis of data set 1 (A) and data set 2 (B) demonstrating the relative importance of individual cytokines in classifying aqueous humor from different study groups. The individual plots represent the relative importance of the cytokines in the overall classification. The vertical axes represent individual cytokines sorted by importance. The horizontal axes represent the average decrease in classification accuracy. Abbreviations: IL=interleukin, TNF=tumor necrosis factor, INF=interferon.
Mentions: The random forest algorithm estimates the importance of a variable in discriminating among study groups. Therefore, we first performed this analysis on both data sets to identify which cytokines seemed key to permitting differentiation among study groups. The results of these analyses are demonstrated in Figure 1, which illustrates the relative importance of individual cytokines in classifying aqueous humor from different study groups.

Bottom Line: We found statistically significant differences for IL-10 and INF-gamma among the study groups.We observed no significant differences concerning all other cytokines evaluated in this study.Perhaps this is a donor-specific activation of the immune privilege in the anterior chamber as an attempt to prevent damage during immune reactions.

View Article: PubMed Central - PubMed

Affiliation: University Eye Hospital and Lions Cornea Bank Baden-Württemberg, Freiburg, Germany. philip.maier@uniklinik-freiburg.de

ABSTRACT

Purpose: Cytokine patterns in the aqueous humor before penetrating keratoplasty might permit us to predict the development of immune reactions. We therefore analyzed ten different cytokines in the aqueous humor of patients before and following penetrating keratoplasty.

Methods: We collected aqueous humor from patients undergoing cataract extraction or penetrating keratoplasty (baseline, n=197), from patients undergoing cataract extraction after penetrating keratoplasty without signs of an endothelial immune reaction (acceptors, n=33), and from patients who underwent irrigation of the anterior chamber due to endothelial immune reactions (rejectors, n=25). We determined interleukin (IL)-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, interferon (INF)-gamma, and tumor necrosis factor-alpha in the aqueous humor, using cytometric bead arrays.

Results: We found statistically significant differences for IL-10 and INF-gamma among the study groups. We observed no significant differences concerning all other cytokines evaluated in this study.

Conclusions: Elevated levels of INF-gamma emphasize its important role in immune reactions following penetrating keratoplasty. Antagonizing INF-gamma might be an option when treating immune reactions. High levels of IL-10 during endothelial immune reactions may reflect a downregulation of the delayed-type hypersensitivity. Perhaps this is a donor-specific activation of the immune privilege in the anterior chamber as an attempt to prevent damage during immune reactions. We plan to investigate, in upcoming prospective trials, whether the analysis of cytokine patterns in the aqueous humor before penetrating keratoplasty will enable us to predict the occurrence of immune reactions.

Show MeSH
Related in: MedlinePlus