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Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography.

van der Meer FJ, Faber DJ, Aalders MC, Poot AA, Vermes I, van Leeuwen TG - Lasers Med Sci (2010)

Bottom Line: We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system.The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient.This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

ABSTRACT
Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optical attenuation coefficient in necrotic cells decreased from 2.2 +/- 0.3 mm(1) to 1.3 +/- 0.6 mm(-1), whereas, in the apoptotic cells, an increase to 6.4 +/- 1.7 mm(-1) was observed. The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment.

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The attenuation coefficient measured in pelleted human fibroblasts, as a function of time. Time was measured in minutes from the point that the cells were forced into necrosis (filled squares) or apoptosis (filled dots). Sham-treated (control) cells show no change in scattering (circles)
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Fig3: The attenuation coefficient measured in pelleted human fibroblasts, as a function of time. Time was measured in minutes from the point that the cells were forced into necrosis (filled squares) or apoptosis (filled dots). Sham-treated (control) cells show no change in scattering (circles)

Mentions: In the experiments described in this study the cells were pelleted and were proven to be viable (data not shown), as in other studies [22–24]. Qualitatively, when compared with untreated cells (Fig. 2a), the cells treated with 200 µM AraC exhibited a temporary increase in OCT signal and a decrease in imaging depth (Fig. 2b). In contrast, cells treated with 10% ethanol showed an increase in imaging depth (Fig. 2c). These observations are quantified by the measurement of µt of the samples in time as presented in Fig. 3. The AraC-treated cells show an initial increase in µt, followed by a decrease, ending up below the level of the control cells. Treatment with 10% ethanol (EtOH) results in an immediate decrease in backscatter, compared to untreated control cells.Fig. 2


Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography.

van der Meer FJ, Faber DJ, Aalders MC, Poot AA, Vermes I, van Leeuwen TG - Lasers Med Sci (2010)

The attenuation coefficient measured in pelleted human fibroblasts, as a function of time. Time was measured in minutes from the point that the cells were forced into necrosis (filled squares) or apoptosis (filled dots). Sham-treated (control) cells show no change in scattering (circles)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2807596&req=5

Fig3: The attenuation coefficient measured in pelleted human fibroblasts, as a function of time. Time was measured in minutes from the point that the cells were forced into necrosis (filled squares) or apoptosis (filled dots). Sham-treated (control) cells show no change in scattering (circles)
Mentions: In the experiments described in this study the cells were pelleted and were proven to be viable (data not shown), as in other studies [22–24]. Qualitatively, when compared with untreated cells (Fig. 2a), the cells treated with 200 µM AraC exhibited a temporary increase in OCT signal and a decrease in imaging depth (Fig. 2b). In contrast, cells treated with 10% ethanol showed an increase in imaging depth (Fig. 2c). These observations are quantified by the measurement of µt of the samples in time as presented in Fig. 3. The AraC-treated cells show an initial increase in µt, followed by a decrease, ending up below the level of the control cells. Treatment with 10% ethanol (EtOH) results in an immediate decrease in backscatter, compared to untreated control cells.Fig. 2

Bottom Line: We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system.The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient.This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

ABSTRACT
Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optical attenuation coefficient in necrotic cells decreased from 2.2 +/- 0.3 mm(1) to 1.3 +/- 0.6 mm(-1), whereas, in the apoptotic cells, an increase to 6.4 +/- 1.7 mm(-1) was observed. The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment.

Show MeSH
Related in: MedlinePlus