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Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

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Related in: MedlinePlus

Growth potential of PESPs from different gestational ages posttransplantation.E42-old PESPs possessed the maximal growth potential. There were significant difference between E42 and E35 (p = 0.001), E42 and E70 (p = 0.001), E42 and E91 (p = 0.001). The number of animals at each time point was 12. There was no significant difference between E42 and E56 (p = 0.21).
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pone-0008717-g006: Growth potential of PESPs from different gestational ages posttransplantation.E42-old PESPs possessed the maximal growth potential. There were significant difference between E42 and E35 (p = 0.001), E42 and E70 (p = 0.001), E42 and E91 (p = 0.001). The number of animals at each time point was 12. There was no significant difference between E42 and E56 (p = 0.21).

Mentions: Generally, the growth potential of PESPs increases firstly and then decreases along with its gestational age. PESPs from E42 showed the maximal growth potential (47.1±6.3 mm2) that was significantly higher than that exhibited by tissues harvested at other gestational ages (Figure 6).


Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

Growth potential of PESPs from different gestational ages posttransplantation.E42-old PESPs possessed the maximal growth potential. There were significant difference between E42 and E35 (p = 0.001), E42 and E70 (p = 0.001), E42 and E91 (p = 0.001). The number of animals at each time point was 12. There was no significant difference between E42 and E56 (p = 0.21).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2807464&req=5

pone-0008717-g006: Growth potential of PESPs from different gestational ages posttransplantation.E42-old PESPs possessed the maximal growth potential. There were significant difference between E42 and E35 (p = 0.001), E42 and E70 (p = 0.001), E42 and E91 (p = 0.001). The number of animals at each time point was 12. There was no significant difference between E42 and E56 (p = 0.21).
Mentions: Generally, the growth potential of PESPs increases firstly and then decreases along with its gestational age. PESPs from E42 showed the maximal growth potential (47.1±6.3 mm2) that was significantly higher than that exhibited by tissues harvested at other gestational ages (Figure 6).

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

Show MeSH
Related in: MedlinePlus