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Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

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Related in: MedlinePlus

The teratoma-like structure of E42-old PESPs after 6-week implantation.Cartilage tissue and cutaneous tissue were found in neoregenerative tissues (HE, ×200 for larger photo, ×400 for smaller photo).
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pone-0008717-g005: The teratoma-like structure of E42-old PESPs after 6-week implantation.Cartilage tissue and cutaneous tissue were found in neoregenerative tissues (HE, ×200 for larger photo, ×400 for smaller photo).

Mentions: The possibility of PESPs differentiation into teratoma was tested in our experiments. The histological examination of implanted skin precursors after 12 weeks of implantation showed that PESPs obtained from E42 could differentiate into both cutaneous tissues and cartilage tissue (2 out of 12 mice, 3 out of 50 grafts) (Figure 5). No teratoma was found following implantation of in E56 or later skin precursor grafts.


Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

The teratoma-like structure of E42-old PESPs after 6-week implantation.Cartilage tissue and cutaneous tissue were found in neoregenerative tissues (HE, ×200 for larger photo, ×400 for smaller photo).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2807464&req=5

pone-0008717-g005: The teratoma-like structure of E42-old PESPs after 6-week implantation.Cartilage tissue and cutaneous tissue were found in neoregenerative tissues (HE, ×200 for larger photo, ×400 for smaller photo).
Mentions: The possibility of PESPs differentiation into teratoma was tested in our experiments. The histological examination of implanted skin precursors after 12 weeks of implantation showed that PESPs obtained from E42 could differentiate into both cutaneous tissues and cartilage tissue (2 out of 12 mice, 3 out of 50 grafts) (Figure 5). No teratoma was found following implantation of in E56 or later skin precursor grafts.

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

Show MeSH
Related in: MedlinePlus