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Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

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Related in: MedlinePlus

The immunohistological staining of neoregenerative tissues after 6–12 week implantation of E56 PESPs.(a) Cytokeratin MNF116 positive cells were located in epithenium (12-week postimplantation). (b) Cytokeratin MNF116 positive cells were located in hair follicle sheet (6-week postimplantation). (c) Vimentin 9 positive cells were located in the dermis around hair follicles (6-week postimplantation). (d) Vimentin 9 positive cells were located in the deep dermis tissue under the host tissue (12-week postimplantation).
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pone-0008717-g004: The immunohistological staining of neoregenerative tissues after 6–12 week implantation of E56 PESPs.(a) Cytokeratin MNF116 positive cells were located in epithenium (12-week postimplantation). (b) Cytokeratin MNF116 positive cells were located in hair follicle sheet (6-week postimplantation). (c) Vimentin 9 positive cells were located in the dermis around hair follicles (6-week postimplantation). (d) Vimentin 9 positive cells were located in the deep dermis tissue under the host tissue (12-week postimplantation).

Mentions: Fontana stain showed that there were melanin cells in the neoregenerative epidermis (Figure 3a). Furthermore, the neoregenerative epithenium and hair follicle sheet cells were positive for anti-cytokeratin MNF116 (Figures 4a and 4b). Dermal cells around hair follicles and in the deep dermal tissue were positive for anti-vementin 9 (Figures 4c and 4d). Neo-regenerative tissues were completely fused with host mouse tissues.


Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

The immunohistological staining of neoregenerative tissues after 6–12 week implantation of E56 PESPs.(a) Cytokeratin MNF116 positive cells were located in epithenium (12-week postimplantation). (b) Cytokeratin MNF116 positive cells were located in hair follicle sheet (6-week postimplantation). (c) Vimentin 9 positive cells were located in the dermis around hair follicles (6-week postimplantation). (d) Vimentin 9 positive cells were located in the deep dermis tissue under the host tissue (12-week postimplantation).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2807464&req=5

pone-0008717-g004: The immunohistological staining of neoregenerative tissues after 6–12 week implantation of E56 PESPs.(a) Cytokeratin MNF116 positive cells were located in epithenium (12-week postimplantation). (b) Cytokeratin MNF116 positive cells were located in hair follicle sheet (6-week postimplantation). (c) Vimentin 9 positive cells were located in the dermis around hair follicles (6-week postimplantation). (d) Vimentin 9 positive cells were located in the deep dermis tissue under the host tissue (12-week postimplantation).
Mentions: Fontana stain showed that there were melanin cells in the neoregenerative epidermis (Figure 3a). Furthermore, the neoregenerative epithenium and hair follicle sheet cells were positive for anti-cytokeratin MNF116 (Figures 4a and 4b). Dermal cells around hair follicles and in the deep dermal tissue were positive for anti-vementin 9 (Figures 4c and 4d). Neo-regenerative tissues were completely fused with host mouse tissues.

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

Show MeSH
Related in: MedlinePlus