Limits...
Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

Show MeSH

Related in: MedlinePlus

The growth of E56-old PESPs after implantation.(a) The PESPs were grafted on the wound and protected by the adult white BAMA pini-pig skin. (b and c) The wound was healed first by host mouse epidermis 2 and 3 weeks postimplantation. (d and e) After 4 weeks of implantation, black color became visible. Finally, the black hair grew out. (f) The dermal side of the black after 6-week implantation.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2807464&req=5

pone-0008717-g002: The growth of E56-old PESPs after implantation.(a) The PESPs were grafted on the wound and protected by the adult white BAMA pini-pig skin. (b and c) The wound was healed first by host mouse epidermis 2 and 3 weeks postimplantation. (d and e) After 4 weeks of implantation, black color became visible. Finally, the black hair grew out. (f) The dermal side of the black after 6-week implantation.

Mentions: In the model of implantation of PESPs, the wound was healed first by mouse epidermal cells (Figures 2b and 2c). However, 2 to 3 weeks posttransplantation, some black spots and nodules could be observed and touched, respectively, under the healed wound. Black spots and hairs could be observed in all PESPs in 4–6 weeks after transplantation (Figures 2d and 2e). One granule of skin precursor could form a skin island like a sucker (Figure 2f). During 8th–12th week posttransplantation, the skin islands had been fusing together. From 8 weeks posttransplantation, the black hairs could be easily found in the wound area (Figure 2e). The growth speed of all implanted skin precursors obviously slowed down after 12 weeks posttransplantation. There was no significant difference in gross morphology of posttransplants between any two gestational age groups, except the time of appearance of the black color and the black hairs in the wound area (Table 1).


Embryonic porcine skin precursors can successfully develop into integrated skin without teratoma formation posttransplantation in nude mouse model.

Huang Z, Yang J, Luo G, Gan C, Cheng W, Yuan S, Peng X, Tan J, Wang X, Hu J, Yang S, Reisner Y, Ge L, Wei H, Cheng P, Wu J - PLoS ONE (2010)

The growth of E56-old PESPs after implantation.(a) The PESPs were grafted on the wound and protected by the adult white BAMA pini-pig skin. (b and c) The wound was healed first by host mouse epidermis 2 and 3 weeks postimplantation. (d and e) After 4 weeks of implantation, black color became visible. Finally, the black hair grew out. (f) The dermal side of the black after 6-week implantation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2807464&req=5

pone-0008717-g002: The growth of E56-old PESPs after implantation.(a) The PESPs were grafted on the wound and protected by the adult white BAMA pini-pig skin. (b and c) The wound was healed first by host mouse epidermis 2 and 3 weeks postimplantation. (d and e) After 4 weeks of implantation, black color became visible. Finally, the black hair grew out. (f) The dermal side of the black after 6-week implantation.
Mentions: In the model of implantation of PESPs, the wound was healed first by mouse epidermal cells (Figures 2b and 2c). However, 2 to 3 weeks posttransplantation, some black spots and nodules could be observed and touched, respectively, under the healed wound. Black spots and hairs could be observed in all PESPs in 4–6 weeks after transplantation (Figures 2d and 2e). One granule of skin precursor could form a skin island like a sucker (Figure 2f). During 8th–12th week posttransplantation, the skin islands had been fusing together. From 8 weeks posttransplantation, the black hairs could be easily found in the wound area (Figure 2e). The growth speed of all implanted skin precursors obviously slowed down after 12 weeks posttransplantation. There was no significant difference in gross morphology of posttransplants between any two gestational age groups, except the time of appearance of the black color and the black hairs in the wound area (Table 1).

Bottom Line: PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later.In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages.Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
How to improve the wound healing quality of severe burn patients is still a challenge due to lack of skin appendages and rete ridges, no matter how much progress has been made in the fields of either stem cell or tissue engineering. We thus systematically studied the growth potential and differentiation capacity of porcine embryonic skin precursors. Implantation of embryonic skin precursors (PESPs) of different gestational ages in nude mice can generate the integrity skin, including epidermis, dermis and skin appendages, such as sweat gland, hair follicle, sebaceous gland, etc.. PESPs of embryonic day 42 possess the maximal growth potential, while, the safe window time of PESPs transplantation for prevention of teratoma risk is E56 or later. In conclusion, PESPs can form the 3 dimensional structures of skin with all necessary skin appendages. Our data strongly indicate that porcine embryonic skin precursors harvested from E56 of minipig may provide new hope for high-quality healing of extensive burns and traumas.

Show MeSH
Related in: MedlinePlus