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Survivin gene levels in the peripheral blood of patients with gastric cancer independently predict survival.

Bertazza L, Mocellin S, Marchet A, Pilati P, Gabrieli J, Scalerta R, Nitti D - J Transl Med (2009)

Bottom Line: In contrast with previously published data, the transcript abundance of CEA, CK19 and VEGF was not associated with patients' clinical outcome.Gene expression levels of Survivin add significant prognostic value to the current TNM staging system.The validation of these findings in larger prospective and multicentric series might lead to the implementation of this biomarker in the routine clinical setting in order to optimize risk stratification and ultimately personalize the therapeutic management of these patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Oncological & Surgical Sciences, Section of Clinica Chirurgica 2, University of Padova, via Giustiniani 2, 35128, Padua, Italy. loris.bertazza@unipd.it

ABSTRACT

Background: The detection of circulating tumor cells (CTC) is considered a promising tool for improving risk stratification in patients with solid tumors. We investigated on whether the expression of CTC related genes adds any prognostic power to the TNM staging system in patients with gastric carcinoma.

Methods: Seventy patients with TNM stage I to IV gastric carcinoma were retrospectively enrolled. Peripheral blood samples were tested by means of quantitative real time PCR (qrtPCR) for the expression of four CTC related genes: carcinoembryonic antigen (CEA), cytokeratin-19 (CK19), vascular endothelial growth factor (VEGF) and Survivin (BIRC5).

Results: Gene expression of Survivin, CK19, CEA and VEGF was higher than in normal controls in 98.6%, 97.1%, 42.9% and 38.6% of cases, respectively, suggesting a potential diagnostic value of both Survivin and CK19. At multivariable survival analysis, TNM staging and Survivin mRNA levels were retained as independent prognostic factors, demonstrating that Survivin expression in the peripheral blood adds prognostic information to the TNM system. In contrast with previously published data, the transcript abundance of CEA, CK19 and VEGF was not associated with patients' clinical outcome.

Conclusions: Gene expression levels of Survivin add significant prognostic value to the current TNM staging system. The validation of these findings in larger prospective and multicentric series might lead to the implementation of this biomarker in the routine clinical setting in order to optimize risk stratification and ultimately personalize the therapeutic management of these patients.

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Gene expression levels of the four genes of interest in N87 human gastric cancer cells (as measured by quantitative real time PCR: see text for details). The natural logarithm of the expression levels is reported on the y axis: the axis origin (0) represents the reference sample (called calibrator) with which all experimental samples are compared in the relative quantification method (see text for details).
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Figure 1: Gene expression levels of the four genes of interest in N87 human gastric cancer cells (as measured by quantitative real time PCR: see text for details). The natural logarithm of the expression levels is reported on the y axis: the axis origin (0) represents the reference sample (called calibrator) with which all experimental samples are compared in the relative quantification method (see text for details).

Mentions: In the N87 cell line, mRNA levels of the CEA, CK19, Survivin, and VEGF genes were highly expressed (Figure 1): therefore, this gastric carcinoma cell line represented a valid positive control for our experiments.


Survivin gene levels in the peripheral blood of patients with gastric cancer independently predict survival.

Bertazza L, Mocellin S, Marchet A, Pilati P, Gabrieli J, Scalerta R, Nitti D - J Transl Med (2009)

Gene expression levels of the four genes of interest in N87 human gastric cancer cells (as measured by quantitative real time PCR: see text for details). The natural logarithm of the expression levels is reported on the y axis: the axis origin (0) represents the reference sample (called calibrator) with which all experimental samples are compared in the relative quantification method (see text for details).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2807427&req=5

Figure 1: Gene expression levels of the four genes of interest in N87 human gastric cancer cells (as measured by quantitative real time PCR: see text for details). The natural logarithm of the expression levels is reported on the y axis: the axis origin (0) represents the reference sample (called calibrator) with which all experimental samples are compared in the relative quantification method (see text for details).
Mentions: In the N87 cell line, mRNA levels of the CEA, CK19, Survivin, and VEGF genes were highly expressed (Figure 1): therefore, this gastric carcinoma cell line represented a valid positive control for our experiments.

Bottom Line: In contrast with previously published data, the transcript abundance of CEA, CK19 and VEGF was not associated with patients' clinical outcome.Gene expression levels of Survivin add significant prognostic value to the current TNM staging system.The validation of these findings in larger prospective and multicentric series might lead to the implementation of this biomarker in the routine clinical setting in order to optimize risk stratification and ultimately personalize the therapeutic management of these patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Oncological & Surgical Sciences, Section of Clinica Chirurgica 2, University of Padova, via Giustiniani 2, 35128, Padua, Italy. loris.bertazza@unipd.it

ABSTRACT

Background: The detection of circulating tumor cells (CTC) is considered a promising tool for improving risk stratification in patients with solid tumors. We investigated on whether the expression of CTC related genes adds any prognostic power to the TNM staging system in patients with gastric carcinoma.

Methods: Seventy patients with TNM stage I to IV gastric carcinoma were retrospectively enrolled. Peripheral blood samples were tested by means of quantitative real time PCR (qrtPCR) for the expression of four CTC related genes: carcinoembryonic antigen (CEA), cytokeratin-19 (CK19), vascular endothelial growth factor (VEGF) and Survivin (BIRC5).

Results: Gene expression of Survivin, CK19, CEA and VEGF was higher than in normal controls in 98.6%, 97.1%, 42.9% and 38.6% of cases, respectively, suggesting a potential diagnostic value of both Survivin and CK19. At multivariable survival analysis, TNM staging and Survivin mRNA levels were retained as independent prognostic factors, demonstrating that Survivin expression in the peripheral blood adds prognostic information to the TNM system. In contrast with previously published data, the transcript abundance of CEA, CK19 and VEGF was not associated with patients' clinical outcome.

Conclusions: Gene expression levels of Survivin add significant prognostic value to the current TNM staging system. The validation of these findings in larger prospective and multicentric series might lead to the implementation of this biomarker in the routine clinical setting in order to optimize risk stratification and ultimately personalize the therapeutic management of these patients.

Show MeSH
Related in: MedlinePlus