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dSir2 mediates the increased spontaneous physical activity in flies on calorie restriction.

Parashar V, Rogina B - Aging (Albany NY) (2009)

Bottom Line: Calorie restriction (CR) is the most effective way to increase life span and delay the onset of age-related symptoms in animals.We have previously reported that CR affects a variety of physiological phenotypes in flies and results in dramatic behavioral, physical and demographic changes.Here we show effects of low and high calorie levels on the spontaneous physical activity of flies.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Developmental Biology, School of Medicine, University of Connecticut Health Center, Farmington, CT 06030-3301, USA.

ABSTRACT
Calorie restriction (CR) is the most effective way to increase life span and delay the onset of age-related symptoms in animals. We have previously reported that CR affects a variety of physiological phenotypes in flies and results in dramatic behavioral, physical and demographic changes. Here we show effects of low and high calorie levels on the spontaneous physical activity of flies. Wild type flies maintained on a low calorie diet exhibit higher spontaneous activity compared to flies on higher calorie diets. This increase is dependent on the presence of Sir2 since a low calorie diet does not increase the activity of dSir2 flies. Similarly, increasing dSir2 activity by feeding flies resveratrol, a CR mimetic, increases spontaneous physical activity of flies on high caloric food. In Drosophila, spontaneous physical activity therefore closely mimics life span in its dependence on Sir2.

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Low calorie diet is associated with increased spontaneous physical activity of Drosophila. Sum of 24-hour                                            spontaneous physical activity of Canton-S male (A) and female                                            (B) flies on a low (0.5X) and a high (1.5X) calorie food based on                                            collected data for days 4 and 9. Both male and female flies on 0.5X food                                            have increased spontaneous physical activity compared to the flies on l.5X                                            food. The mobility was based on the mean mobility of 3 vials with 10 male                                            or 10 female flies each, and expressed as mean total activity per vial                                            during 24 hours +/- SEM. (C) Mean total 24 hours spontaneous                                            activity of male and female CS flies on 0.5X and 1.5X food at age 4                                            and 9 expressed per vial. Statistical significance was determined by using                                            two-tailed Student's t-test for independent samples.
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Figure 1: Low calorie diet is associated with increased spontaneous physical activity of Drosophila. Sum of 24-hour spontaneous physical activity of Canton-S male (A) and female (B) flies on a low (0.5X) and a high (1.5X) calorie food based on collected data for days 4 and 9. Both male and female flies on 0.5X food have increased spontaneous physical activity compared to the flies on l.5X food. The mobility was based on the mean mobility of 3 vials with 10 male or 10 female flies each, and expressed as mean total activity per vial during 24 hours +/- SEM. (C) Mean total 24 hours spontaneous activity of male and female CS flies on 0.5X and 1.5X food at age 4 and 9 expressed per vial. Statistical significance was determined by using two-tailed Student's t-test for independent samples.

Mentions: We previously reported that the 24-hour activity of flies (mixed genders) depends on the calorie content of the food, with increased activity associated with a low calorie diet [47]. In order to determine gender-specific effects of diet on spontaneous physical activity, male and female Canton-S wild type flies were aged together on food with low (0.5X) and high (1.5X) calorie contents post-eclosion. The caloric content of 0.5X food is 50% that of the 1.0X food, and flies kept on 0.5X food have extended lifespan [42,43]. From 3 days of age, we recorded spontaneous activity of flies separated by gender in 3 groups of 10 male or female flies. Each cohort was transferred into the population monitors with 0.5X or 1.5X food and placed in a temperature controlled incubator set at 25°C, with a 12 hour light-dark cycle. Using computer controlled population activity monitors, we were able to monitor spontaneous physical activity of the flies throughout most of their first 10 days of life. The days when the flies were passed to new vials were not used for calculations. There is a significant increase in the 24 hour spontaneous physical activity (total) observed in male flies kept at 0.5X food compared to flies kept on 1.5 food level at age 4 days [t(1, 58) = 7.21; η2= 0.47], Figure 1A, Table 1. A similar statistical difference in the total spontaneous activity of male flies kept on 0.5X and 1.5X was found at age 9, [t(1, 58) = 6.59; η2= 0.43], suggesting that the difference in activity is not only associated with very young age of 4 days. Female flies show a significant increases in spontaneous physical activity associated with low calorie food at age 4, [t(1, 58) = 9.15; η2= 0.59], but not at age 9, Figure 1B, Table 1. However, this result may be due to the high standard errors of the means observed in recorded mobility for female flies on 0.5X and 1.5X food levels at age 9. We also examined if there is a gender specific difference in the levels of physical activity of the flies in response to 0.5X vs. 1.5X food levels. There is no significant difference in the activity of the male and female flies on 0.5X food levels at both ages, nor on 1.5X food level at age 9. However, there is a statistically significant increase in the spontaneous physical activity of the female flies on 1.5X food levels at age 4 [t(1, 47.2) = -4.575; p<0.001; η2= 0.265]. Interestingly, the activity of the female flies is higher than males at both food levels at age 4, but lower at age 9 days, Figure 1C. These data further confirm that, like mammals, male and female flies respond to a low calorie diet with increased spontaneous physical activity.


dSir2 mediates the increased spontaneous physical activity in flies on calorie restriction.

Parashar V, Rogina B - Aging (Albany NY) (2009)

Low calorie diet is associated with increased spontaneous physical activity of Drosophila. Sum of 24-hour                                            spontaneous physical activity of Canton-S male (A) and female                                            (B) flies on a low (0.5X) and a high (1.5X) calorie food based on                                            collected data for days 4 and 9. Both male and female flies on 0.5X food                                            have increased spontaneous physical activity compared to the flies on l.5X                                            food. The mobility was based on the mean mobility of 3 vials with 10 male                                            or 10 female flies each, and expressed as mean total activity per vial                                            during 24 hours +/- SEM. (C) Mean total 24 hours spontaneous                                            activity of male and female CS flies on 0.5X and 1.5X food at age 4                                            and 9 expressed per vial. Statistical significance was determined by using                                            two-tailed Student's t-test for independent samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2806034&req=5

Figure 1: Low calorie diet is associated with increased spontaneous physical activity of Drosophila. Sum of 24-hour spontaneous physical activity of Canton-S male (A) and female (B) flies on a low (0.5X) and a high (1.5X) calorie food based on collected data for days 4 and 9. Both male and female flies on 0.5X food have increased spontaneous physical activity compared to the flies on l.5X food. The mobility was based on the mean mobility of 3 vials with 10 male or 10 female flies each, and expressed as mean total activity per vial during 24 hours +/- SEM. (C) Mean total 24 hours spontaneous activity of male and female CS flies on 0.5X and 1.5X food at age 4 and 9 expressed per vial. Statistical significance was determined by using two-tailed Student's t-test for independent samples.
Mentions: We previously reported that the 24-hour activity of flies (mixed genders) depends on the calorie content of the food, with increased activity associated with a low calorie diet [47]. In order to determine gender-specific effects of diet on spontaneous physical activity, male and female Canton-S wild type flies were aged together on food with low (0.5X) and high (1.5X) calorie contents post-eclosion. The caloric content of 0.5X food is 50% that of the 1.0X food, and flies kept on 0.5X food have extended lifespan [42,43]. From 3 days of age, we recorded spontaneous activity of flies separated by gender in 3 groups of 10 male or female flies. Each cohort was transferred into the population monitors with 0.5X or 1.5X food and placed in a temperature controlled incubator set at 25°C, with a 12 hour light-dark cycle. Using computer controlled population activity monitors, we were able to monitor spontaneous physical activity of the flies throughout most of their first 10 days of life. The days when the flies were passed to new vials were not used for calculations. There is a significant increase in the 24 hour spontaneous physical activity (total) observed in male flies kept at 0.5X food compared to flies kept on 1.5 food level at age 4 days [t(1, 58) = 7.21; η2= 0.47], Figure 1A, Table 1. A similar statistical difference in the total spontaneous activity of male flies kept on 0.5X and 1.5X was found at age 9, [t(1, 58) = 6.59; η2= 0.43], suggesting that the difference in activity is not only associated with very young age of 4 days. Female flies show a significant increases in spontaneous physical activity associated with low calorie food at age 4, [t(1, 58) = 9.15; η2= 0.59], but not at age 9, Figure 1B, Table 1. However, this result may be due to the high standard errors of the means observed in recorded mobility for female flies on 0.5X and 1.5X food levels at age 9. We also examined if there is a gender specific difference in the levels of physical activity of the flies in response to 0.5X vs. 1.5X food levels. There is no significant difference in the activity of the male and female flies on 0.5X food levels at both ages, nor on 1.5X food level at age 9. However, there is a statistically significant increase in the spontaneous physical activity of the female flies on 1.5X food levels at age 4 [t(1, 47.2) = -4.575; p<0.001; η2= 0.265]. Interestingly, the activity of the female flies is higher than males at both food levels at age 4, but lower at age 9 days, Figure 1C. These data further confirm that, like mammals, male and female flies respond to a low calorie diet with increased spontaneous physical activity.

Bottom Line: Calorie restriction (CR) is the most effective way to increase life span and delay the onset of age-related symptoms in animals.We have previously reported that CR affects a variety of physiological phenotypes in flies and results in dramatic behavioral, physical and demographic changes.Here we show effects of low and high calorie levels on the spontaneous physical activity of flies.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Developmental Biology, School of Medicine, University of Connecticut Health Center, Farmington, CT 06030-3301, USA.

ABSTRACT
Calorie restriction (CR) is the most effective way to increase life span and delay the onset of age-related symptoms in animals. We have previously reported that CR affects a variety of physiological phenotypes in flies and results in dramatic behavioral, physical and demographic changes. Here we show effects of low and high calorie levels on the spontaneous physical activity of flies. Wild type flies maintained on a low calorie diet exhibit higher spontaneous activity compared to flies on higher calorie diets. This increase is dependent on the presence of Sir2 since a low calorie diet does not increase the activity of dSir2 flies. Similarly, increasing dSir2 activity by feeding flies resveratrol, a CR mimetic, increases spontaneous physical activity of flies on high caloric food. In Drosophila, spontaneous physical activity therefore closely mimics life span in its dependence on Sir2.

Show MeSH
Related in: MedlinePlus