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Expression sites of colligin 2 in glioma blood vessels.

Mustafa D, van der Weiden M, Zheng P, Nigg A, Luider TM, Kros JM - Brain Pathol. (2009)

Bottom Line: Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels.There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon.We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory of Neuro-oncology and Clinical Proteomics, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GD Rotterdam, The Netherlands.

ABSTRACT
In a previous study using state-of-the-art proteomic techniques, we identified colligin 2 (HSP47) as a glioma blood vessel-specific protein. In the present study we precisely localized the expression of colligin 2 in the blood vessels of diffusely infiltrating gliomas and relate the expression to the distinct cellular components of the vessels by using multiple immunolabeling and confocal microscopy. We grouped the glioma blood vessels into morphological categories ranging from normal looking capillaries to vessels with hypertrophic and sclerotic changes. The expression patterns of various markers of endothelial and pericytic differentiation were correlated with the position of the cells in the vessels and the expression of colligin 2. We found that colligin 2 is expressed in all categories of glioma blood vessels in cells with endothelial and pericytic lineage. Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels. There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

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Related in: MedlinePlus

Confocal images of glioma blood vessels for the expression of colligin 2 by activated (CD105-positive) endothelial cells. A–E. Double immunolabeling for CD105/colligin 2 in hypertrophied blood vessel. CD105 and colligin 2 are expressed by endothelial cells while the cells around the endothelium express only colligin 2 (arrows) (panel B: green = CD105; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole; panel E: merged picture).
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fig08: Confocal images of glioma blood vessels for the expression of colligin 2 by activated (CD105-positive) endothelial cells. A–E. Double immunolabeling for CD105/colligin 2 in hypertrophied blood vessel. CD105 and colligin 2 are expressed by endothelial cells while the cells around the endothelium express only colligin 2 (arrows) (panel B: green = CD105; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole; panel E: merged picture).

Mentions: The high resolution of the confocal microscope enables to detail the expression of the various markers at the level of individual cells. In all distinguished types of glioma blood vessels, the lumina-lining endothelial cells were positive for CD31 and CD34, while none of the other cellular components were positive for these markers (Figure 7). CD105, a marker for activated endothelial cells, was expressed by the endothelial cells in all different types of blood vessels in the glioma samples (Figure 8). The percentage of endothelial cells that expressed colligin 2 varied between the blood vessels in the same sample and ranged between high-, and hardly any expression. In some endothelial cells, expression levels of colligin 2 was the same as that of CD31 or CD34, while other cells showed a very low level of expression.


Expression sites of colligin 2 in glioma blood vessels.

Mustafa D, van der Weiden M, Zheng P, Nigg A, Luider TM, Kros JM - Brain Pathol. (2009)

Confocal images of glioma blood vessels for the expression of colligin 2 by activated (CD105-positive) endothelial cells. A–E. Double immunolabeling for CD105/colligin 2 in hypertrophied blood vessel. CD105 and colligin 2 are expressed by endothelial cells while the cells around the endothelium express only colligin 2 (arrows) (panel B: green = CD105; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole; panel E: merged picture).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2805918&req=5

fig08: Confocal images of glioma blood vessels for the expression of colligin 2 by activated (CD105-positive) endothelial cells. A–E. Double immunolabeling for CD105/colligin 2 in hypertrophied blood vessel. CD105 and colligin 2 are expressed by endothelial cells while the cells around the endothelium express only colligin 2 (arrows) (panel B: green = CD105; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole; panel E: merged picture).
Mentions: The high resolution of the confocal microscope enables to detail the expression of the various markers at the level of individual cells. In all distinguished types of glioma blood vessels, the lumina-lining endothelial cells were positive for CD31 and CD34, while none of the other cellular components were positive for these markers (Figure 7). CD105, a marker for activated endothelial cells, was expressed by the endothelial cells in all different types of blood vessels in the glioma samples (Figure 8). The percentage of endothelial cells that expressed colligin 2 varied between the blood vessels in the same sample and ranged between high-, and hardly any expression. In some endothelial cells, expression levels of colligin 2 was the same as that of CD31 or CD34, while other cells showed a very low level of expression.

Bottom Line: Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels.There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon.We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory of Neuro-oncology and Clinical Proteomics, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GD Rotterdam, The Netherlands.

ABSTRACT
In a previous study using state-of-the-art proteomic techniques, we identified colligin 2 (HSP47) as a glioma blood vessel-specific protein. In the present study we precisely localized the expression of colligin 2 in the blood vessels of diffusely infiltrating gliomas and relate the expression to the distinct cellular components of the vessels by using multiple immunolabeling and confocal microscopy. We grouped the glioma blood vessels into morphological categories ranging from normal looking capillaries to vessels with hypertrophic and sclerotic changes. The expression patterns of various markers of endothelial and pericytic differentiation were correlated with the position of the cells in the vessels and the expression of colligin 2. We found that colligin 2 is expressed in all categories of glioma blood vessels in cells with endothelial and pericytic lineage. Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels. There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

Show MeSH
Related in: MedlinePlus