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Expression sites of colligin 2 in glioma blood vessels.

Mustafa D, van der Weiden M, Zheng P, Nigg A, Luider TM, Kros JM - Brain Pathol. (2009)

Bottom Line: Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels.There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon.We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory of Neuro-oncology and Clinical Proteomics, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GD Rotterdam, The Netherlands.

ABSTRACT
In a previous study using state-of-the-art proteomic techniques, we identified colligin 2 (HSP47) as a glioma blood vessel-specific protein. In the present study we precisely localized the expression of colligin 2 in the blood vessels of diffusely infiltrating gliomas and relate the expression to the distinct cellular components of the vessels by using multiple immunolabeling and confocal microscopy. We grouped the glioma blood vessels into morphological categories ranging from normal looking capillaries to vessels with hypertrophic and sclerotic changes. The expression patterns of various markers of endothelial and pericytic differentiation were correlated with the position of the cells in the vessels and the expression of colligin 2. We found that colligin 2 is expressed in all categories of glioma blood vessels in cells with endothelial and pericytic lineage. Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels. There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

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Confocal images of glioma blood vessels for the expression of colligin 2 in Mib-1 positive cells. A–E. Double immunolabeling for Mib-1/colligin 2 in a small blood vessel. A fraction of colligin 2 positive endothelial cells are Mib-1 positive (arrows) [panel B: green = collagen I; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole (DAPI); panel E: merged picture]. F–J. Double immunolabeling for Mib-1/colligin 2 in glomeruloid vessels in glioma. Some Mib-1 positive endothelium-surrounding cells express colligin 2 (arrows) while others do not (stars) (panel G: green = Mib-1; panel H: red = colligin 2; panel I: blue = DAPI; panel J: merged picture).
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fig11: Confocal images of glioma blood vessels for the expression of colligin 2 in Mib-1 positive cells. A–E. Double immunolabeling for Mib-1/colligin 2 in a small blood vessel. A fraction of colligin 2 positive endothelial cells are Mib-1 positive (arrows) [panel B: green = collagen I; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole (DAPI); panel E: merged picture]. F–J. Double immunolabeling for Mib-1/colligin 2 in glomeruloid vessels in glioma. Some Mib-1 positive endothelium-surrounding cells express colligin 2 (arrows) while others do not (stars) (panel G: green = Mib-1; panel H: red = colligin 2; panel I: blue = DAPI; panel J: merged picture).

Mentions: Because colligin 2 is the chaperon for the collagen types I and IV, we included these proteins in our investigations. There appeared to be an overlap in expression of colligin 2 and collagen type I and IV. However, not all colligin 2-positive cells showed expression of collagen. For instance, some cells in the glomeruloid and small blood vessels exclusively expressed colligin 2 (Figure 10). The endothelial cells remained negative for collagens I and IV. Noticeably, the individual colligin 2-expressing cells found around the blood vessels never expressed the two collagens (Figure 10). The proliferation-related marker Mib-1 was combined with colligin 2 and co-expression was seen in some endothelial cells of small and hypertrophied vessels. In the glomeruloid vessels Mib-1 expression was seen in both colligin 2 positive and negative cells (Figure 11). The glial fibrillary acidic protein (GFAP)-positive astrocytes residing outside the blood vessels remained negative for colligin 2, but a very low percentage of these cells participating in the glioma blood vessel formation were found to co-express colligin 2 (Figure 12).


Expression sites of colligin 2 in glioma blood vessels.

Mustafa D, van der Weiden M, Zheng P, Nigg A, Luider TM, Kros JM - Brain Pathol. (2009)

Confocal images of glioma blood vessels for the expression of colligin 2 in Mib-1 positive cells. A–E. Double immunolabeling for Mib-1/colligin 2 in a small blood vessel. A fraction of colligin 2 positive endothelial cells are Mib-1 positive (arrows) [panel B: green = collagen I; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole (DAPI); panel E: merged picture]. F–J. Double immunolabeling for Mib-1/colligin 2 in glomeruloid vessels in glioma. Some Mib-1 positive endothelium-surrounding cells express colligin 2 (arrows) while others do not (stars) (panel G: green = Mib-1; panel H: red = colligin 2; panel I: blue = DAPI; panel J: merged picture).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig11: Confocal images of glioma blood vessels for the expression of colligin 2 in Mib-1 positive cells. A–E. Double immunolabeling for Mib-1/colligin 2 in a small blood vessel. A fraction of colligin 2 positive endothelial cells are Mib-1 positive (arrows) [panel B: green = collagen I; panel C: red = colligin 2; panel D: blue = 4′,6-diamidino-2-phenylindole (DAPI); panel E: merged picture]. F–J. Double immunolabeling for Mib-1/colligin 2 in glomeruloid vessels in glioma. Some Mib-1 positive endothelium-surrounding cells express colligin 2 (arrows) while others do not (stars) (panel G: green = Mib-1; panel H: red = colligin 2; panel I: blue = DAPI; panel J: merged picture).
Mentions: Because colligin 2 is the chaperon for the collagen types I and IV, we included these proteins in our investigations. There appeared to be an overlap in expression of colligin 2 and collagen type I and IV. However, not all colligin 2-positive cells showed expression of collagen. For instance, some cells in the glomeruloid and small blood vessels exclusively expressed colligin 2 (Figure 10). The endothelial cells remained negative for collagens I and IV. Noticeably, the individual colligin 2-expressing cells found around the blood vessels never expressed the two collagens (Figure 10). The proliferation-related marker Mib-1 was combined with colligin 2 and co-expression was seen in some endothelial cells of small and hypertrophied vessels. In the glomeruloid vessels Mib-1 expression was seen in both colligin 2 positive and negative cells (Figure 11). The glial fibrillary acidic protein (GFAP)-positive astrocytes residing outside the blood vessels remained negative for colligin 2, but a very low percentage of these cells participating in the glioma blood vessel formation were found to co-express colligin 2 (Figure 12).

Bottom Line: Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels.There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon.We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory of Neuro-oncology and Clinical Proteomics, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GD Rotterdam, The Netherlands.

ABSTRACT
In a previous study using state-of-the-art proteomic techniques, we identified colligin 2 (HSP47) as a glioma blood vessel-specific protein. In the present study we precisely localized the expression of colligin 2 in the blood vessels of diffusely infiltrating gliomas and relate the expression to the distinct cellular components of the vessels by using multiple immunolabeling and confocal microscopy. We grouped the glioma blood vessels into morphological categories ranging from normal looking capillaries to vessels with hypertrophic and sclerotic changes. The expression patterns of various markers of endothelial and pericytic differentiation were correlated with the position of the cells in the vessels and the expression of colligin 2. We found that colligin 2 is expressed in all categories of glioma blood vessels in cells with endothelial and pericytic lineage. Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels. There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

Show MeSH
Related in: MedlinePlus