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Expression sites of colligin 2 in glioma blood vessels.

Mustafa D, van der Weiden M, Zheng P, Nigg A, Luider TM, Kros JM - Brain Pathol. (2009)

Bottom Line: Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels.There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon.We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory of Neuro-oncology and Clinical Proteomics, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GD Rotterdam, The Netherlands.

ABSTRACT
In a previous study using state-of-the-art proteomic techniques, we identified colligin 2 (HSP47) as a glioma blood vessel-specific protein. In the present study we precisely localized the expression of colligin 2 in the blood vessels of diffusely infiltrating gliomas and relate the expression to the distinct cellular components of the vessels by using multiple immunolabeling and confocal microscopy. We grouped the glioma blood vessels into morphological categories ranging from normal looking capillaries to vessels with hypertrophic and sclerotic changes. The expression patterns of various markers of endothelial and pericytic differentiation were correlated with the position of the cells in the vessels and the expression of colligin 2. We found that colligin 2 is expressed in all categories of glioma blood vessels in cells with endothelial and pericytic lineage. Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels. There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

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Expression of the various markers in normal brain. A–D. CD31, CD34 and collagen IV are all expressed in capillaries. αSMA is variably expressed (panels A, B,D: ×40; panel C: ×20). E–J. CD31, CD34, αSMA, collagen IV are expressed in small vessels in normal brain; endosialin and colligin 2 are not expressed (E,F–H,J: ×40; I: ×20).
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fig01: Expression of the various markers in normal brain. A–D. CD31, CD34 and collagen IV are all expressed in capillaries. αSMA is variably expressed (panels A, B,D: ×40; panel C: ×20). E–J. CD31, CD34, αSMA, collagen IV are expressed in small vessels in normal brain; endosialin and colligin 2 are not expressed (E,F–H,J: ×40; I: ×20).

Mentions: The endothelial cells of small and medium-sized vessels in the samples of a normal brain showed immunopositivity for CD31 and CD34 but not for αSMA (Table 3). The mural cells were variably positive for αSMA. None of the cells of the blood vessels in normal brain were positive for CD105, NG2, endosialin or colligin 2 (Figure 1 and Table 3).


Expression sites of colligin 2 in glioma blood vessels.

Mustafa D, van der Weiden M, Zheng P, Nigg A, Luider TM, Kros JM - Brain Pathol. (2009)

Expression of the various markers in normal brain. A–D. CD31, CD34 and collagen IV are all expressed in capillaries. αSMA is variably expressed (panels A, B,D: ×40; panel C: ×20). E–J. CD31, CD34, αSMA, collagen IV are expressed in small vessels in normal brain; endosialin and colligin 2 are not expressed (E,F–H,J: ×40; I: ×20).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2805918&req=5

fig01: Expression of the various markers in normal brain. A–D. CD31, CD34 and collagen IV are all expressed in capillaries. αSMA is variably expressed (panels A, B,D: ×40; panel C: ×20). E–J. CD31, CD34, αSMA, collagen IV are expressed in small vessels in normal brain; endosialin and colligin 2 are not expressed (E,F–H,J: ×40; I: ×20).
Mentions: The endothelial cells of small and medium-sized vessels in the samples of a normal brain showed immunopositivity for CD31 and CD34 but not for αSMA (Table 3). The mural cells were variably positive for αSMA. None of the cells of the blood vessels in normal brain were positive for CD105, NG2, endosialin or colligin 2 (Figure 1 and Table 3).

Bottom Line: Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels.There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon.We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory of Neuro-oncology and Clinical Proteomics, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GD Rotterdam, The Netherlands.

ABSTRACT
In a previous study using state-of-the-art proteomic techniques, we identified colligin 2 (HSP47) as a glioma blood vessel-specific protein. In the present study we precisely localized the expression of colligin 2 in the blood vessels of diffusely infiltrating gliomas and relate the expression to the distinct cellular components of the vessels by using multiple immunolabeling and confocal microscopy. We grouped the glioma blood vessels into morphological categories ranging from normal looking capillaries to vessels with hypertrophic and sclerotic changes. The expression patterns of various markers of endothelial and pericytic differentiation were correlated with the position of the cells in the vessels and the expression of colligin 2. We found that colligin 2 is expressed in all categories of glioma blood vessels in cells with endothelial and pericytic lineage. Expression of colligin 2 was also found in cells scattered around blood vessels and in few glial fibrillary acidic protein-positive cells within the blood vessels. There is overlap in the expression of colligin 2 and the collagens type I and IV for which colligin 2 is a chaperon. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis.

Show MeSH
Related in: MedlinePlus