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Genetic variance of Derzsy's disease strains isolated in Poland.

Woźniakowski G, Kozdruń W, Samorek-Salamonowicz E - J Mol Genet Med (2009)

Bottom Line: The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan.Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study.The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Viral Diseases, National Veterinary Research Institute, Al Partyzantów 57, 24-100 Puławy, Poland.

ABSTRACT
The aim of this study was the assessment of the genetic variance of Derzy's disease (GPV) strains isolated from cases occurring in Poland. The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan. The observed genetic variance of the aminoacid sequence within the group of Polish strains was low and reached 5% of the overall analysed sequence. Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study. The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of the Polish strains of GPV and MDPV on the basis of VP3 amino acid sequence. The values on the axis multiplied by 100% give the identity coefficient of strains.
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Figure 4: Phylogenetic tree of the Polish strains of GPV and MDPV on the basis of VP3 amino acid sequence. The values on the axis multiplied by 100% give the identity coefficient of strains.

Mentions: The first group consisted of the strains originating from Hungary, Polish vaccine strain PIW-82, Polish field strain 14/02 and GPV486 English vaccine strain. The second group was represented by FY, 99-1222, HEB, ZD, QTH and ZZ strain, whereas the third group was represented largely by Taiwanese strains (98-1218, TWL, 82-38, SP, 82-322). The strains originating from France and one Taiwanese strain 92-217 fell into the fourth group. Polish field strains of GPV: 14/01, 9/03, 24/03, 33/03, 54/03, 8/07, 14/07, 16/07 and G/07 were classified into two different groups. Seven of them, 14/01, 9/03, 24/03, 33/03, 14/07, 16/07 and G/07, were classified into the fifth group but into two different branches even though their sequences were similar. Chinese strains DB-Q and DB-H formed Group 8. Two strains of identical sequence, 54/03 and 8/07, were assigned to Group 9. The strains of Muscovy duck parvovirus FM, GD, TWK fell into a common group (Group 7), irrespective of their geographical origin. Moreover, on the basis of the analysis of the amino acid sequence of VP3 protein the GPV strains isolated in Poland, 24/03, 33/03 and 14/02, were classified into a common phylogenetic group (Figure 4). The high homology of the VP3 amino acid sequence was also found among strains 9/03, G/07, 16/07 and PIW-82. The most divergent was the sequence of the Polish MFP strain that showed 5% variation. The strains 14/01 and 14/07 as well as 54/03 and 8/07 were assigned into two different groups. The VP3 amino acid sequence of the MDPV FM differed by 16% as compared with other strains isolated in Poland. Despite this variation, overall, the data obtained in the present study suggest a common origin of the GPV and FM MDPV strains.


Genetic variance of Derzsy's disease strains isolated in Poland.

Woźniakowski G, Kozdruń W, Samorek-Salamonowicz E - J Mol Genet Med (2009)

Phylogenetic tree of the Polish strains of GPV and MDPV on the basis of VP3 amino acid sequence. The values on the axis multiplied by 100% give the identity coefficient of strains.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2805842&req=5

Figure 4: Phylogenetic tree of the Polish strains of GPV and MDPV on the basis of VP3 amino acid sequence. The values on the axis multiplied by 100% give the identity coefficient of strains.
Mentions: The first group consisted of the strains originating from Hungary, Polish vaccine strain PIW-82, Polish field strain 14/02 and GPV486 English vaccine strain. The second group was represented by FY, 99-1222, HEB, ZD, QTH and ZZ strain, whereas the third group was represented largely by Taiwanese strains (98-1218, TWL, 82-38, SP, 82-322). The strains originating from France and one Taiwanese strain 92-217 fell into the fourth group. Polish field strains of GPV: 14/01, 9/03, 24/03, 33/03, 54/03, 8/07, 14/07, 16/07 and G/07 were classified into two different groups. Seven of them, 14/01, 9/03, 24/03, 33/03, 14/07, 16/07 and G/07, were classified into the fifth group but into two different branches even though their sequences were similar. Chinese strains DB-Q and DB-H formed Group 8. Two strains of identical sequence, 54/03 and 8/07, were assigned to Group 9. The strains of Muscovy duck parvovirus FM, GD, TWK fell into a common group (Group 7), irrespective of their geographical origin. Moreover, on the basis of the analysis of the amino acid sequence of VP3 protein the GPV strains isolated in Poland, 24/03, 33/03 and 14/02, were classified into a common phylogenetic group (Figure 4). The high homology of the VP3 amino acid sequence was also found among strains 9/03, G/07, 16/07 and PIW-82. The most divergent was the sequence of the Polish MFP strain that showed 5% variation. The strains 14/01 and 14/07 as well as 54/03 and 8/07 were assigned into two different groups. The VP3 amino acid sequence of the MDPV FM differed by 16% as compared with other strains isolated in Poland. Despite this variation, overall, the data obtained in the present study suggest a common origin of the GPV and FM MDPV strains.

Bottom Line: The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan.Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study.The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Viral Diseases, National Veterinary Research Institute, Al Partyzantów 57, 24-100 Puławy, Poland.

ABSTRACT
The aim of this study was the assessment of the genetic variance of Derzy's disease (GPV) strains isolated from cases occurring in Poland. The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan. The observed genetic variance of the aminoacid sequence within the group of Polish strains was low and reached 5% of the overall analysed sequence. Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study. The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

No MeSH data available.


Related in: MedlinePlus