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Genetic variance of Derzsy's disease strains isolated in Poland.

Woźniakowski G, Kozdruń W, Samorek-Salamonowicz E - J Mol Genet Med (2009)

Bottom Line: The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan.Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study.The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Viral Diseases, National Veterinary Research Institute, Al Partyzantów 57, 24-100 Puławy, Poland.

ABSTRACT
The aim of this study was the assessment of the genetic variance of Derzy's disease (GPV) strains isolated from cases occurring in Poland. The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan. The observed genetic variance of the aminoacid sequence within the group of Polish strains was low and reached 5% of the overall analysed sequence. Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study. The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of GPV and MDPV strains on the basis of VP2 encoding region (vacc.=vaccine strain)
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Figure 1: Phylogenetic tree of GPV and MDPV strains on the basis of VP2 encoding region (vacc.=vaccine strain)

Mentions: All studied DNA samples of GPV strains, except MDPV FM, showed one characteristic amplicon of ˜1760bp representing amplified VP2. The VP3 region amplification from GPV strains as well as from MDPV FM strain presented a specific PCR product of ˜1600bp. Sequenced contigs of VP2 and VP3 regions were compared with other accessible GPV and MDPV sequences available in NCBI Genebank. The Polish sequences of VP2 and VP3 regions were aligned with homologous sequences from Hungary, France, China, Taiwan, England and Germany. On the basis of the analysis of the nucleotide sequence encoding VP2 protein six different phylogenetic groups of GPV were distinguished (Figure 1). In the first group the strains originating from the territory of Hungary, GPV486 English strain and Chinese strains fell into the common lineage. The French strains fell into the second group while the third group was represented by strains from the territory of China as well as the Taiwanese TWL strain. The Polish strains constituted the fourth and sixth group (Figure 1). Three Polish strains, 24/03, 33/03 and PIW-82, presented the highly homologous VP2 sequence while six other strains, 54/03, MFP, 14/01, 8/07, 14/07 and G/07, established a separate group. Differences between the fourth and sixth group were confirmed by the analysis of the predicted amino acid sequence for the VP2 protein and were presented as a separate tree (Figure 2). The analysis of the amino acid sequence of VP2 protein of the Polish strains revealed the occurrence of two phylogenetic groups. The strains 33/03, 24/03 and PIW-82 were classified into the first group, while the strains 14/07 and G/07 fell into the second group. The homology coefficient of the amino acid sequence of the Polish strains ranged from 83 to 100%. The strains of Muscovy duck parvovirus (MDPV) originating from China, Taiwan and Hungary constituted a common fifth group (Figure 1). The topology of the particular branches of the VP2-based phylogenetic tree revealed that the studied strains possessed a common ancestor.


Genetic variance of Derzsy's disease strains isolated in Poland.

Woźniakowski G, Kozdruń W, Samorek-Salamonowicz E - J Mol Genet Med (2009)

Phylogenetic tree of GPV and MDPV strains on the basis of VP2 encoding region (vacc.=vaccine strain)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2805842&req=5

Figure 1: Phylogenetic tree of GPV and MDPV strains on the basis of VP2 encoding region (vacc.=vaccine strain)
Mentions: All studied DNA samples of GPV strains, except MDPV FM, showed one characteristic amplicon of ˜1760bp representing amplified VP2. The VP3 region amplification from GPV strains as well as from MDPV FM strain presented a specific PCR product of ˜1600bp. Sequenced contigs of VP2 and VP3 regions were compared with other accessible GPV and MDPV sequences available in NCBI Genebank. The Polish sequences of VP2 and VP3 regions were aligned with homologous sequences from Hungary, France, China, Taiwan, England and Germany. On the basis of the analysis of the nucleotide sequence encoding VP2 protein six different phylogenetic groups of GPV were distinguished (Figure 1). In the first group the strains originating from the territory of Hungary, GPV486 English strain and Chinese strains fell into the common lineage. The French strains fell into the second group while the third group was represented by strains from the territory of China as well as the Taiwanese TWL strain. The Polish strains constituted the fourth and sixth group (Figure 1). Three Polish strains, 24/03, 33/03 and PIW-82, presented the highly homologous VP2 sequence while six other strains, 54/03, MFP, 14/01, 8/07, 14/07 and G/07, established a separate group. Differences between the fourth and sixth group were confirmed by the analysis of the predicted amino acid sequence for the VP2 protein and were presented as a separate tree (Figure 2). The analysis of the amino acid sequence of VP2 protein of the Polish strains revealed the occurrence of two phylogenetic groups. The strains 33/03, 24/03 and PIW-82 were classified into the first group, while the strains 14/07 and G/07 fell into the second group. The homology coefficient of the amino acid sequence of the Polish strains ranged from 83 to 100%. The strains of Muscovy duck parvovirus (MDPV) originating from China, Taiwan and Hungary constituted a common fifth group (Figure 1). The topology of the particular branches of the VP2-based phylogenetic tree revealed that the studied strains possessed a common ancestor.

Bottom Line: The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan.Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study.The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Viral Diseases, National Veterinary Research Institute, Al Partyzantów 57, 24-100 Puławy, Poland.

ABSTRACT
The aim of this study was the assessment of the genetic variance of Derzy's disease (GPV) strains isolated from cases occurring in Poland. The nucleotide and predicted aminoacid sequences of VP2 and VP3 surface proteins of the Polish GPV strains were compared with other strains previously isolated in Hungary, France, Germany, China and Taiwan. The observed genetic variance of the aminoacid sequence within the group of Polish strains was low and reached 5% of the overall analysed sequence. Considerable differences in aminoacid sequence were found in the case of Polish field GPV strains and Muscovy duck parvovirus strain MDPV FM which was also analysed in this study. The conducted investigations confirmed the presupposition that Polish GPV strains and strains previously isolated in Hungary and France share a common origin.

No MeSH data available.


Related in: MedlinePlus