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Lrmp/Jaw1 is expressed in sweet, bitter, and umami receptor-expressing cells.

Shindo Y, Kim MR, Miura H, Yuuki T, Kanda T, Hino A, Kusakabe Y - Chem. Senses (2010)

Bottom Line: Although the main components of this cascade have been identified, the candidate regulators of them in taste tissues are still unclear.In addition to this specific expression patterns, we found that Lrmp/Jaw1 is associated with type III IP3 receptor (IP3R3) via its coiled-coil domain in the COS7 heterologous expression system.These results raise the possibility that Lrmp/Jaw1 interacts with IP3R3 in taste cells and suggest an important role for Lrmp/Jaw1 in the IP3-Ca2+ signal cascade in sweet, bitter, and umami taste signal transduction.

View Article: PubMed Central - PubMed

Affiliation: Sensory and Cognitive Food Sciences, National Food Research Institute, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan.

ABSTRACT
Inositol 1,4,5-triphosphate-mediated calcium (IP3-Ca2+) signal cascade is an essential process in sweet, bitter, and umami taste signal transduction. Although the main components of this cascade have been identified, the candidate regulators of them in taste tissues are still unclear. In an effort to identify genes involved in taste signal transduction, we found that a gene encoding lymphoid-restricted membrane protein (Lrmp/Jaw1) was expressed in mouse taste tissues. Here we report that Lrmp/Jaw1 is specifically expressed in sweet, bitter, and umami taste receptor-expressing cells of mouse circumvallate, foliate, and fungiform papillae. In addition to this specific expression patterns, we found that Lrmp/Jaw1 is associated with type III IP3 receptor (IP3R3) via its coiled-coil domain in the COS7 heterologous expression system. These results raise the possibility that Lrmp/Jaw1 interacts with IP3R3 in taste cells and suggest an important role for Lrmp/Jaw1 in the IP3-Ca2+ signal cascade in sweet, bitter, and umami taste signal transduction.

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Cellular distribution for mRNA of Lrmp/Jaw1 gene in taste buds. Mouse CV, FL, and FF papillae slices (5 μm) were used for in situ hybridization. The broken lines indicate the outline of each taste bud. The expressions of the genes were visualized by NBT–BCIP. The scale bars indicate 50 μm.
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fig1: Cellular distribution for mRNA of Lrmp/Jaw1 gene in taste buds. Mouse CV, FL, and FF papillae slices (5 μm) were used for in situ hybridization. The broken lines indicate the outline of each taste bud. The expressions of the genes were visualized by NBT–BCIP. The scale bars indicate 50 μm.

Mentions: To identify genes related to taste signal transduction, we isolated and sequenced 600 clones of subtracted cDNA library derived from mouse CV papillae and tongue epithelium. According to sequence annotations, approximately 50 genes predicted to code membrane proteins or signal transduction–related proteins were selected for subsequent in situ hybridization analyses using mouse CV sections. As a result of this process, we found that Lrmp/Jaw1 was distinctly expressed in a specific subset of taste cells (Figure 1). To investigate the expression patterns in detail, we performed in situ hybridization using FL and FF in addition to CV papillae sections. Robust signals were observed in a subset of taste cells of all taste buds in each papilla but not in surrounding tongue epithelium (Figure 1).


Lrmp/Jaw1 is expressed in sweet, bitter, and umami receptor-expressing cells.

Shindo Y, Kim MR, Miura H, Yuuki T, Kanda T, Hino A, Kusakabe Y - Chem. Senses (2010)

Cellular distribution for mRNA of Lrmp/Jaw1 gene in taste buds. Mouse CV, FL, and FF papillae slices (5 μm) were used for in situ hybridization. The broken lines indicate the outline of each taste bud. The expressions of the genes were visualized by NBT–BCIP. The scale bars indicate 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2805811&req=5

fig1: Cellular distribution for mRNA of Lrmp/Jaw1 gene in taste buds. Mouse CV, FL, and FF papillae slices (5 μm) were used for in situ hybridization. The broken lines indicate the outline of each taste bud. The expressions of the genes were visualized by NBT–BCIP. The scale bars indicate 50 μm.
Mentions: To identify genes related to taste signal transduction, we isolated and sequenced 600 clones of subtracted cDNA library derived from mouse CV papillae and tongue epithelium. According to sequence annotations, approximately 50 genes predicted to code membrane proteins or signal transduction–related proteins were selected for subsequent in situ hybridization analyses using mouse CV sections. As a result of this process, we found that Lrmp/Jaw1 was distinctly expressed in a specific subset of taste cells (Figure 1). To investigate the expression patterns in detail, we performed in situ hybridization using FL and FF in addition to CV papillae sections. Robust signals were observed in a subset of taste cells of all taste buds in each papilla but not in surrounding tongue epithelium (Figure 1).

Bottom Line: Although the main components of this cascade have been identified, the candidate regulators of them in taste tissues are still unclear.In addition to this specific expression patterns, we found that Lrmp/Jaw1 is associated with type III IP3 receptor (IP3R3) via its coiled-coil domain in the COS7 heterologous expression system.These results raise the possibility that Lrmp/Jaw1 interacts with IP3R3 in taste cells and suggest an important role for Lrmp/Jaw1 in the IP3-Ca2+ signal cascade in sweet, bitter, and umami taste signal transduction.

View Article: PubMed Central - PubMed

Affiliation: Sensory and Cognitive Food Sciences, National Food Research Institute, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan.

ABSTRACT
Inositol 1,4,5-triphosphate-mediated calcium (IP3-Ca2+) signal cascade is an essential process in sweet, bitter, and umami taste signal transduction. Although the main components of this cascade have been identified, the candidate regulators of them in taste tissues are still unclear. In an effort to identify genes involved in taste signal transduction, we found that a gene encoding lymphoid-restricted membrane protein (Lrmp/Jaw1) was expressed in mouse taste tissues. Here we report that Lrmp/Jaw1 is specifically expressed in sweet, bitter, and umami taste receptor-expressing cells of mouse circumvallate, foliate, and fungiform papillae. In addition to this specific expression patterns, we found that Lrmp/Jaw1 is associated with type III IP3 receptor (IP3R3) via its coiled-coil domain in the COS7 heterologous expression system. These results raise the possibility that Lrmp/Jaw1 interacts with IP3R3 in taste cells and suggest an important role for Lrmp/Jaw1 in the IP3-Ca2+ signal cascade in sweet, bitter, and umami taste signal transduction.

Show MeSH