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Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa.

Mok FS, Thiyagarajan V, Qian PY - Proteome Sci (2009)

Bottom Line: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite.Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles.The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

View Article: PubMed Central - HTML - PubMed

Affiliation: KAUST Global Partnership Program, Department of Biology/Atmospheric, Marine and Coastal Environment Program, The Hong Kong University of Science and Technology, Hong Kong SAR, China.

ABSTRACT

Background: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.

Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).

Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

No MeSH data available.


Related in: MedlinePlus

Differential expression of proteins during the development of spionid Pseudopolydora vexillosa larvae. COM: competent larvae, META: newly metamorphosed juveniles. (A) Number of protein spots observed in each stage. (B) Number of protein spots showing a 2-fold increase in spot volume between successive stages, and (C) number of protein spots that showed a 2-fold decrease in spot volume between successive stages. (D) Number of spots that showed a significant difference in both their presence/absence and their spot volumes between successive stages by Student's t test (p < 0.02), n = 2.
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Figure 5: Differential expression of proteins during the development of spionid Pseudopolydora vexillosa larvae. COM: competent larvae, META: newly metamorphosed juveniles. (A) Number of protein spots observed in each stage. (B) Number of protein spots showing a 2-fold increase in spot volume between successive stages, and (C) number of protein spots that showed a 2-fold decrease in spot volume between successive stages. (D) Number of spots that showed a significant difference in both their presence/absence and their spot volumes between successive stages by Student's t test (p < 0.02), n = 2.

Mentions: Ninety-nine new protein spots, concentrated in the pI range of 4.5 to 7, appeared in the competent larvae that were not evident in pre-competent larvae; no new protein spots were detected in metamorphosed juveniles (Fig. 2, 5A). A visual comparison of the protein profiles from competent larvae and metamorphosed juveniles showed that the positions of the protein spots in these two stages were unchanged (see Fig. 2B, 2C). Between the competent larvae and the newly-metamorphosed juveniles, 149 proteins were up-regulated and 11 were down-regulated (Fig. 5B, 5C). The protein gels of the competent larvae showed 124 protein spots that were significantly different (in terms of both presence and volumes of the protein spots) from those of the pre-competent larvae, but only 34 spots that were significantly different from the newly-metamorphosed juveniles (t2 = 6.967, p < 0.02, Fig. 5D).


Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa.

Mok FS, Thiyagarajan V, Qian PY - Proteome Sci (2009)

Differential expression of proteins during the development of spionid Pseudopolydora vexillosa larvae. COM: competent larvae, META: newly metamorphosed juveniles. (A) Number of protein spots observed in each stage. (B) Number of protein spots showing a 2-fold increase in spot volume between successive stages, and (C) number of protein spots that showed a 2-fold decrease in spot volume between successive stages. (D) Number of spots that showed a significant difference in both their presence/absence and their spot volumes between successive stages by Student's t test (p < 0.02), n = 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2803448&req=5

Figure 5: Differential expression of proteins during the development of spionid Pseudopolydora vexillosa larvae. COM: competent larvae, META: newly metamorphosed juveniles. (A) Number of protein spots observed in each stage. (B) Number of protein spots showing a 2-fold increase in spot volume between successive stages, and (C) number of protein spots that showed a 2-fold decrease in spot volume between successive stages. (D) Number of spots that showed a significant difference in both their presence/absence and their spot volumes between successive stages by Student's t test (p < 0.02), n = 2.
Mentions: Ninety-nine new protein spots, concentrated in the pI range of 4.5 to 7, appeared in the competent larvae that were not evident in pre-competent larvae; no new protein spots were detected in metamorphosed juveniles (Fig. 2, 5A). A visual comparison of the protein profiles from competent larvae and metamorphosed juveniles showed that the positions of the protein spots in these two stages were unchanged (see Fig. 2B, 2C). Between the competent larvae and the newly-metamorphosed juveniles, 149 proteins were up-regulated and 11 were down-regulated (Fig. 5B, 5C). The protein gels of the competent larvae showed 124 protein spots that were significantly different (in terms of both presence and volumes of the protein spots) from those of the pre-competent larvae, but only 34 spots that were significantly different from the newly-metamorphosed juveniles (t2 = 6.967, p < 0.02, Fig. 5D).

Bottom Line: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite.Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles.The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

View Article: PubMed Central - HTML - PubMed

Affiliation: KAUST Global Partnership Program, Department of Biology/Atmospheric, Marine and Coastal Environment Program, The Hong Kong University of Science and Technology, Hong Kong SAR, China.

ABSTRACT

Background: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.

Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).

Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

No MeSH data available.


Related in: MedlinePlus