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Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa.

Mok FS, Thiyagarajan V, Qian PY - Proteome Sci (2009)

Bottom Line: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite.Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles.The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

View Article: PubMed Central - HTML - PubMed

Affiliation: KAUST Global Partnership Program, Department of Biology/Atmospheric, Marine and Coastal Environment Program, The Hong Kong University of Science and Technology, Hong Kong SAR, China.

ABSTRACT

Background: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.

Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).

Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

No MeSH data available.


Related in: MedlinePlus

The mean numbers of protein spots expressed in P. vexillosa pre-competent larvae, competent larvae and newly-metamorphosed juveniles. Each bar represents the mean (± S.D.) of 2 replicate 2-DE gels. Letters indicate significant differences by Tukey HSD posthoc test.
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Figure 3: The mean numbers of protein spots expressed in P. vexillosa pre-competent larvae, competent larvae and newly-metamorphosed juveniles. Each bar represents the mean (± S.D.) of 2 replicate 2-DE gels. Letters indicate significant differences by Tukey HSD posthoc test.

Mentions: Most of the protein spots in the 3 developmental stages (pre-competent larvae, competent larvae, and newly metamorphosed juveniles, Fig. 1) were in the range of pI 4-7 and concentrated in the molecular weight range of 14-90 kDa (Fig. 2). The number of protein spots varied significantly between stages (F2,3 = 383.603, p < 0.001); pre-competent larvae had significantly fewer protein spots (Tukey HSD: p < 0.05; 384 ± 1; mean ± S.D.) than competent larvae (657 ± 4; mean ± S.D.) and newly metamorphosed juveniles (658 ± 5; mean ± S.D.) (Fig. 3).


Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa.

Mok FS, Thiyagarajan V, Qian PY - Proteome Sci (2009)

The mean numbers of protein spots expressed in P. vexillosa pre-competent larvae, competent larvae and newly-metamorphosed juveniles. Each bar represents the mean (± S.D.) of 2 replicate 2-DE gels. Letters indicate significant differences by Tukey HSD posthoc test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2803448&req=5

Figure 3: The mean numbers of protein spots expressed in P. vexillosa pre-competent larvae, competent larvae and newly-metamorphosed juveniles. Each bar represents the mean (± S.D.) of 2 replicate 2-DE gels. Letters indicate significant differences by Tukey HSD posthoc test.
Mentions: Most of the protein spots in the 3 developmental stages (pre-competent larvae, competent larvae, and newly metamorphosed juveniles, Fig. 1) were in the range of pI 4-7 and concentrated in the molecular weight range of 14-90 kDa (Fig. 2). The number of protein spots varied significantly between stages (F2,3 = 383.603, p < 0.001); pre-competent larvae had significantly fewer protein spots (Tukey HSD: p < 0.05; 384 ± 1; mean ± S.D.) than competent larvae (657 ± 4; mean ± S.D.) and newly metamorphosed juveniles (658 ± 5; mean ± S.D.) (Fig. 3).

Bottom Line: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite.Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles.The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

View Article: PubMed Central - HTML - PubMed

Affiliation: KAUST Global Partnership Program, Department of Biology/Atmospheric, Marine and Coastal Environment Program, The Hong Kong University of Science and Technology, Hong Kong SAR, China.

ABSTRACT

Background: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.

Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).

Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.

No MeSH data available.


Related in: MedlinePlus