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Regulation of Immune Responses by the Activating and Inhibitory Myeloid-Associate Immunoglobuline-Like Receptors (MAIR) (CD300).

Shibuya A, Nakahashi-Oda C, Tahara-Hanaoka S - Immune Netw (2009)

Bottom Line: We and others identified paired activating and inhibitory immunoglobulin-like receptors, designated myeloid-associated immunoglobulin-like receptors (MAIR) (CD300).MAIR family receptors are preferentially expressed on myeloid cells, including macrophages, dendritic cells, granulocytes, and bone-marrow-derived cultured mast cells, and a subset of B cells and regulate activation of these cells.Thus, MAIR plays an important role in innate immunity mediated by myeloid cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan.

ABSTRACT
Activating and inhibitory cell surface receptors play important roles in regulation of immune responses. Recent progress has demonstrated that many inhibitory receptors pair with activating, as well as inhibitory, isoforms, both of whose genes are located in small clusters on a chromosome. We and others identified paired activating and inhibitory immunoglobulin-like receptors, designated myeloid-associated immunoglobulin-like receptors (MAIR) (CD300). MAIR is a multigene family consisting of nine genes on a small segment of mouse chromosome 11. MAIR family receptors are preferentially expressed on myeloid cells, including macrophages, dendritic cells, granulocytes, and bone-marrow-derived cultured mast cells, and a subset of B cells and regulate activation of these cells. Thus, MAIR plays an important role in innate immunity mediated by myeloid cells.

No MeSH data available.


Molecular phylogenetic tree of MAIR gene family. Phylogenetic analysis of the MAIR family genes was performed by using the UPGMA method of GENETYX-MAC software (Software develoment, Tokyo, Japan). MAIR-II, MAIR-III, MAIR-VII and MAIR-VIII contained a charged amino acid in the transmembrane portion, and some of them associate with ITAM-bearing adaptor. MAIR-I and MAIR-V contains ITIM in the cytoplasmic portion.
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Figure 3: Molecular phylogenetic tree of MAIR gene family. Phylogenetic analysis of the MAIR family genes was performed by using the UPGMA method of GENETYX-MAC software (Software develoment, Tokyo, Japan). MAIR-II, MAIR-III, MAIR-VII and MAIR-VIII contained a charged amino acid in the transmembrane portion, and some of them associate with ITAM-bearing adaptor. MAIR-I and MAIR-V contains ITIM in the cytoplasmic portion.

Mentions: We cloned all the full-length cDNAs of the MAIR family genes other than MAIR-I and MAIR-II by PCR from the spleen of C57BL/6 mice and designated them as MAIR-III to MAIR-IX, based on a phylogenetic tree analysis (Fig. 3). We found that one of the genes, MAIR-IV, has a short cytoplasmic tail (24 aa) with no signaling motif and a negatively charged glutamic acid (E) in its transmembrane (TM) region (Fig. 1). The Ig-like domain of MAIR-IV in the extracellular portion has 91% identity with that of MAIR-V at the amino acid level. In contrast to MAIR-IV, MAIR-V has a long cytoplasmic tail containing two consensus immunoreceptor tyrosine-based inhibitory motif (ITIM), suggesting that MAIR-IV and MAIR-V constitute paired activating and inhibitory receptors (Fig. 1). MAIR-IV and MAIR-V were also named as CLM5 and CLM1/DIgR2 (11,14,15), respectively. The MAIR family was found to be a murine counterpart of the human CMRF-35 (CD300) family (16-18), which is located on human chromosome 17, syntenic region of mouse chromosome 11 (Fig. 2).


Regulation of Immune Responses by the Activating and Inhibitory Myeloid-Associate Immunoglobuline-Like Receptors (MAIR) (CD300).

Shibuya A, Nakahashi-Oda C, Tahara-Hanaoka S - Immune Netw (2009)

Molecular phylogenetic tree of MAIR gene family. Phylogenetic analysis of the MAIR family genes was performed by using the UPGMA method of GENETYX-MAC software (Software develoment, Tokyo, Japan). MAIR-II, MAIR-III, MAIR-VII and MAIR-VIII contained a charged amino acid in the transmembrane portion, and some of them associate with ITAM-bearing adaptor. MAIR-I and MAIR-V contains ITIM in the cytoplasmic portion.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2803305&req=5

Figure 3: Molecular phylogenetic tree of MAIR gene family. Phylogenetic analysis of the MAIR family genes was performed by using the UPGMA method of GENETYX-MAC software (Software develoment, Tokyo, Japan). MAIR-II, MAIR-III, MAIR-VII and MAIR-VIII contained a charged amino acid in the transmembrane portion, and some of them associate with ITAM-bearing adaptor. MAIR-I and MAIR-V contains ITIM in the cytoplasmic portion.
Mentions: We cloned all the full-length cDNAs of the MAIR family genes other than MAIR-I and MAIR-II by PCR from the spleen of C57BL/6 mice and designated them as MAIR-III to MAIR-IX, based on a phylogenetic tree analysis (Fig. 3). We found that one of the genes, MAIR-IV, has a short cytoplasmic tail (24 aa) with no signaling motif and a negatively charged glutamic acid (E) in its transmembrane (TM) region (Fig. 1). The Ig-like domain of MAIR-IV in the extracellular portion has 91% identity with that of MAIR-V at the amino acid level. In contrast to MAIR-IV, MAIR-V has a long cytoplasmic tail containing two consensus immunoreceptor tyrosine-based inhibitory motif (ITIM), suggesting that MAIR-IV and MAIR-V constitute paired activating and inhibitory receptors (Fig. 1). MAIR-IV and MAIR-V were also named as CLM5 and CLM1/DIgR2 (11,14,15), respectively. The MAIR family was found to be a murine counterpart of the human CMRF-35 (CD300) family (16-18), which is located on human chromosome 17, syntenic region of mouse chromosome 11 (Fig. 2).

Bottom Line: We and others identified paired activating and inhibitory immunoglobulin-like receptors, designated myeloid-associated immunoglobulin-like receptors (MAIR) (CD300).MAIR family receptors are preferentially expressed on myeloid cells, including macrophages, dendritic cells, granulocytes, and bone-marrow-derived cultured mast cells, and a subset of B cells and regulate activation of these cells.Thus, MAIR plays an important role in innate immunity mediated by myeloid cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan.

ABSTRACT
Activating and inhibitory cell surface receptors play important roles in regulation of immune responses. Recent progress has demonstrated that many inhibitory receptors pair with activating, as well as inhibitory, isoforms, both of whose genes are located in small clusters on a chromosome. We and others identified paired activating and inhibitory immunoglobulin-like receptors, designated myeloid-associated immunoglobulin-like receptors (MAIR) (CD300). MAIR is a multigene family consisting of nine genes on a small segment of mouse chromosome 11. MAIR family receptors are preferentially expressed on myeloid cells, including macrophages, dendritic cells, granulocytes, and bone-marrow-derived cultured mast cells, and a subset of B cells and regulate activation of these cells. Thus, MAIR plays an important role in innate immunity mediated by myeloid cells.

No MeSH data available.