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MICB0106 gene polymorphism is associated with ulcerative colitis in central China.

Li Y, Xia B, Lü M, Ge L, Zhang X - Int J Colorectal Dis (2009)

Bottom Line: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006).The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University School of Medicine, Wuhan, People's Republic of China.

ABSTRACT

Background: The highly polymorphic nonclassical MHC class I chain-related genes A and B (MICA and MICB) encode stress-inducible glycoproteins expressed on various epithelial cells including intestinal epithelial cells. MICA and MICB gene polymorphisms and expressions are associated with autoimmune diseases but not known in ulcerative colitis (UC).

Aims: To investigate the association of MICB exon 2-4 polymorphisms and soluble MICA (sMICA) expression with the susceptibility of UC in central China.

Materials and methods: Genomic DNA was isolated from peripheral blood. The allele frequencies of MICB exon 2-4 were genotyped in 105 UC patients and 213 healthy controls by PCR single-stranded conformation polymorphism method. Thirty-two patients and 32 controls were selected for determining serum sMICA expression by ELISA.

Results: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006). The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.

Conclusions: Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

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a sMICA concentration in UC patients and HC.* The mean sMICA concentration in the serum of 33 UC patients (604.41 ± 480.43 pg/ml) was significantly higher than that in 32 healthy controls (175.37 ± 28.31 pg/ml). Z = −6.967, P = 0.0001. b sMICA concentration in three subgroups of UC patients. The sMICA concentration in the subgroups of MICB0106 carriers, MICA-A5.1 carriers, and non-MICB0106/MICA-A5.1 carriers was 647.64 ± 518.07 pg/ml, 641.76 ± 603.73 pg/ml, and 543.32 ± 343.70 pg/ml, respectively. The differences between sMICA levels among them did not reach statistical significance ()
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Fig2: a sMICA concentration in UC patients and HC.* The mean sMICA concentration in the serum of 33 UC patients (604.41 ± 480.43 pg/ml) was significantly higher than that in 32 healthy controls (175.37 ± 28.31 pg/ml). Z = −6.967, P = 0.0001. b sMICA concentration in three subgroups of UC patients. The sMICA concentration in the subgroups of MICB0106 carriers, MICA-A5.1 carriers, and non-MICB0106/MICA-A5.1 carriers was 647.64 ± 518.07 pg/ml, 641.76 ± 603.73 pg/ml, and 543.32 ± 343.70 pg/ml, respectively. The differences between sMICA levels among them did not reach statistical significance ()

Mentions: In order to investigate sMICA, we selected 33 UC patients and 32 healthy controls randomly from the subjects we had previously. The patients were divided into three subgroups: (1) 12 UC patients carried MICB0106; (2) Eight UC patients carried MICA-A5.1; (3) 13 UC patients carried neither MICB0106 nor MICA-A5.1. Those who carried both MICB0106 and MICA-A5.1 were not included. Tested by a sandwich ELISA, the mean sMICA concentration in the serum of 33 UC patients (604.41 ± 480.43 pg/ml) was 3∼6-fold higher than that in 32 healthy control (175.37 ± 28.31 pg/ml; Fig. 2a). The significant difference reached Z = -6.967, P = 0.0001, which indicates that sMICA was strongly associated with UC.Fig. 2


MICB0106 gene polymorphism is associated with ulcerative colitis in central China.

Li Y, Xia B, Lü M, Ge L, Zhang X - Int J Colorectal Dis (2009)

a sMICA concentration in UC patients and HC.* The mean sMICA concentration in the serum of 33 UC patients (604.41 ± 480.43 pg/ml) was significantly higher than that in 32 healthy controls (175.37 ± 28.31 pg/ml). Z = −6.967, P = 0.0001. b sMICA concentration in three subgroups of UC patients. The sMICA concentration in the subgroups of MICB0106 carriers, MICA-A5.1 carriers, and non-MICB0106/MICA-A5.1 carriers was 647.64 ± 518.07 pg/ml, 641.76 ± 603.73 pg/ml, and 543.32 ± 343.70 pg/ml, respectively. The differences between sMICA levels among them did not reach statistical significance ()
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Related In: Results  -  Collection

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Fig2: a sMICA concentration in UC patients and HC.* The mean sMICA concentration in the serum of 33 UC patients (604.41 ± 480.43 pg/ml) was significantly higher than that in 32 healthy controls (175.37 ± 28.31 pg/ml). Z = −6.967, P = 0.0001. b sMICA concentration in three subgroups of UC patients. The sMICA concentration in the subgroups of MICB0106 carriers, MICA-A5.1 carriers, and non-MICB0106/MICA-A5.1 carriers was 647.64 ± 518.07 pg/ml, 641.76 ± 603.73 pg/ml, and 543.32 ± 343.70 pg/ml, respectively. The differences between sMICA levels among them did not reach statistical significance ()
Mentions: In order to investigate sMICA, we selected 33 UC patients and 32 healthy controls randomly from the subjects we had previously. The patients were divided into three subgroups: (1) 12 UC patients carried MICB0106; (2) Eight UC patients carried MICA-A5.1; (3) 13 UC patients carried neither MICB0106 nor MICA-A5.1. Those who carried both MICB0106 and MICA-A5.1 were not included. Tested by a sandwich ELISA, the mean sMICA concentration in the serum of 33 UC patients (604.41 ± 480.43 pg/ml) was 3∼6-fold higher than that in 32 healthy control (175.37 ± 28.31 pg/ml; Fig. 2a). The significant difference reached Z = -6.967, P = 0.0001, which indicates that sMICA was strongly associated with UC.Fig. 2

Bottom Line: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006).The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University School of Medicine, Wuhan, People's Republic of China.

ABSTRACT

Background: The highly polymorphic nonclassical MHC class I chain-related genes A and B (MICA and MICB) encode stress-inducible glycoproteins expressed on various epithelial cells including intestinal epithelial cells. MICA and MICB gene polymorphisms and expressions are associated with autoimmune diseases but not known in ulcerative colitis (UC).

Aims: To investigate the association of MICB exon 2-4 polymorphisms and soluble MICA (sMICA) expression with the susceptibility of UC in central China.

Materials and methods: Genomic DNA was isolated from peripheral blood. The allele frequencies of MICB exon 2-4 were genotyped in 105 UC patients and 213 healthy controls by PCR single-stranded conformation polymorphism method. Thirty-two patients and 32 controls were selected for determining serum sMICA expression by ELISA.

Results: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006). The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.

Conclusions: Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

Show MeSH
Related in: MedlinePlus