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MICB0106 gene polymorphism is associated with ulcerative colitis in central China.

Li Y, Xia B, Lü M, Ge L, Zhang X - Int J Colorectal Dis (2009)

Bottom Line: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006).The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University School of Medicine, Wuhan, People's Republic of China.

ABSTRACT

Background: The highly polymorphic nonclassical MHC class I chain-related genes A and B (MICA and MICB) encode stress-inducible glycoproteins expressed on various epithelial cells including intestinal epithelial cells. MICA and MICB gene polymorphisms and expressions are associated with autoimmune diseases but not known in ulcerative colitis (UC).

Aims: To investigate the association of MICB exon 2-4 polymorphisms and soluble MICA (sMICA) expression with the susceptibility of UC in central China.

Materials and methods: Genomic DNA was isolated from peripheral blood. The allele frequencies of MICB exon 2-4 were genotyped in 105 UC patients and 213 healthy controls by PCR single-stranded conformation polymorphism method. Thirty-two patients and 32 controls were selected for determining serum sMICA expression by ELISA.

Results: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006). The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.

Conclusions: Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

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PCR-SSCP analysis of MICB allele. a Three distinct banding patterns were observed in exon 2. b Four distinct banding patterns were observed in exon 3. c Only two distinct banding patterns were observed in exon 4. Banding patterns with one or two lanes showed homozygosis, while three or four lanes showed heterozygosis
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Fig1: PCR-SSCP analysis of MICB allele. a Three distinct banding patterns were observed in exon 2. b Four distinct banding patterns were observed in exon 3. c Only two distinct banding patterns were observed in exon 4. Banding patterns with one or two lanes showed homozygosis, while three or four lanes showed heterozygosis

Mentions: MICB alleles were assigned based on the distinct and combined SSCP patterns of exons 2, 3, 4, respectively (Fig. 1a–c). PCR products were directly sequenced as proved by an automated DNA sequencer (ABI PRISM 3730, BigDye terminator v3.1 reagent) using the same primers.Fig. 1


MICB0106 gene polymorphism is associated with ulcerative colitis in central China.

Li Y, Xia B, Lü M, Ge L, Zhang X - Int J Colorectal Dis (2009)

PCR-SSCP analysis of MICB allele. a Three distinct banding patterns were observed in exon 2. b Four distinct banding patterns were observed in exon 3. c Only two distinct banding patterns were observed in exon 4. Banding patterns with one or two lanes showed homozygosis, while three or four lanes showed heterozygosis
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2803256&req=5

Fig1: PCR-SSCP analysis of MICB allele. a Three distinct banding patterns were observed in exon 2. b Four distinct banding patterns were observed in exon 3. c Only two distinct banding patterns were observed in exon 4. Banding patterns with one or two lanes showed homozygosis, while three or four lanes showed heterozygosis
Mentions: MICB alleles were assigned based on the distinct and combined SSCP patterns of exons 2, 3, 4, respectively (Fig. 1a–c). PCR products were directly sequenced as proved by an automated DNA sequencer (ABI PRISM 3730, BigDye terminator v3.1 reagent) using the same primers.Fig. 1

Bottom Line: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006).The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University School of Medicine, Wuhan, People's Republic of China.

ABSTRACT

Background: The highly polymorphic nonclassical MHC class I chain-related genes A and B (MICA and MICB) encode stress-inducible glycoproteins expressed on various epithelial cells including intestinal epithelial cells. MICA and MICB gene polymorphisms and expressions are associated with autoimmune diseases but not known in ulcerative colitis (UC).

Aims: To investigate the association of MICB exon 2-4 polymorphisms and soluble MICA (sMICA) expression with the susceptibility of UC in central China.

Materials and methods: Genomic DNA was isolated from peripheral blood. The allele frequencies of MICB exon 2-4 were genotyped in 105 UC patients and 213 healthy controls by PCR single-stranded conformation polymorphism method. Thirty-two patients and 32 controls were selected for determining serum sMICA expression by ELISA.

Results: Allele frequency of MICB0106 was significantly higher in UC patients than in healthy controls (19.0% vs. 8.9%, corrected P (Pc) = 0.0006), especially in patients with extensive colitis (24.4% vs. 8.9%, Pc = 0.0006), moderate and severe disease (24.1% vs. 8.9%, Pc = 0.0006), extraintestinal manifestations (20.5% vs. 8.9%, Pc = 0.012), male patients (22.1% vs. 8.0%, Pc = 0.006), and patients over the age of 40 years (28.8% vs. 8.3%, Pc = 0.0006). The sMICA level was significantly higher in UC than in healthy controls (604.41 +/- 480.43 pg/ml vs. 175.37 +/- 28.31 pg/ml, P = 0.0001) but not associated with the MICB0106 genotypes.

Conclusions: Overall, MICB0106 allele was positively associated with UC in the Han Chinese in central China. sMICA was highly expressed in UC but not associated with the MICB0106 genotype.

Show MeSH
Related in: MedlinePlus