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Synthesis and expression of CDw75 antigen in human colorectal cancer.

Costa-Nogueira C, Villar-Portela S, Cuevas E, Gil-Martín E, Fernández-Briera A - BMC Cancer (2009)

Bottom Line: Increased ST6Gal I activity has been associated with the alpha(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC).This epitope has been reported to be associated with the progression of gastric and colorectal tumours, hence there are only a few conclusive studies to date.ST6Gal I activity and CDw75 antigen expression were increased in CRC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, Vigo, Spain. cloticn@yahoo.es

ABSTRACT

Background: Increased ST6Gal I activity has been associated with the alpha(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC). Siaalpha(2,6)Galbeta(1,4)GlcNAc sequence, known as CDw75, is a sialylated carbohydrate determinant generated by the ST6Gal I. This epitope has been reported to be associated with the progression of gastric and colorectal tumours, hence there are only a few conclusive studies to date.

Methods: By radioisotopic techniques we evaluated the ST6Gal I activity in healthy, transitional and tumour tissues from 43 patients with CRC. By immunohistochemistry we assessed the CDw75 expression in 25 colorectal adenomas, 43 tumours, 13 transitional and 28 healthy tissues of CRC patients.

Results: ST6Gal I activity was likewise found to be statistically higher in tumour tissue respect to healthy tissue from CRC patients. CDw75 expression was positive in 20% of colorectal adenomas. Furthermore, 70% of tumour specimens and 8.3% of transitional specimens were positive for CDw75 expression, whereas none of the healthy ones showed the presence of the epitope.

Conclusion: The major contribution of this study is the inclusion of data from transitional tissue and the analysis of CDw75 antigen expression in CRC and in colorectal adenomas, little known so far. ST6Gal I activity and CDw75 antigen expression were increased in CRC. Although their comparison did not reach the statistical significance, a great extent of patients showed both, an enhanced tumour ST6Gal I activity and an increased CDw75 expression in the tumour tissue. So, these two variables may play a role in malignant transformation. The expression of CDw75 in colorectal adenomas suggests that this antigen may be a tumour marker in CRC.

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Percentage of positive and negative CDw75 expression in AJCC stages. n = number of specimens.
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Figure 4: Percentage of positive and negative CDw75 expression in AJCC stages. n = number of specimens.

Mentions: TNM and AJCC classifications were used to correlate CDw75 expression (presence or absence and degree of expression) and the tumour stage, and the χ2 test was used to perform the statistical analysis. Although there were no significant differences, highest percentages of CDw75 positive expression in advanced tumour stages were observed (Fig. 4).


Synthesis and expression of CDw75 antigen in human colorectal cancer.

Costa-Nogueira C, Villar-Portela S, Cuevas E, Gil-Martín E, Fernández-Briera A - BMC Cancer (2009)

Percentage of positive and negative CDw75 expression in AJCC stages. n = number of specimens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2803195&req=5

Figure 4: Percentage of positive and negative CDw75 expression in AJCC stages. n = number of specimens.
Mentions: TNM and AJCC classifications were used to correlate CDw75 expression (presence or absence and degree of expression) and the tumour stage, and the χ2 test was used to perform the statistical analysis. Although there were no significant differences, highest percentages of CDw75 positive expression in advanced tumour stages were observed (Fig. 4).

Bottom Line: Increased ST6Gal I activity has been associated with the alpha(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC).This epitope has been reported to be associated with the progression of gastric and colorectal tumours, hence there are only a few conclusive studies to date.ST6Gal I activity and CDw75 antigen expression were increased in CRC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, Vigo, Spain. cloticn@yahoo.es

ABSTRACT

Background: Increased ST6Gal I activity has been associated with the alpha(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC). Siaalpha(2,6)Galbeta(1,4)GlcNAc sequence, known as CDw75, is a sialylated carbohydrate determinant generated by the ST6Gal I. This epitope has been reported to be associated with the progression of gastric and colorectal tumours, hence there are only a few conclusive studies to date.

Methods: By radioisotopic techniques we evaluated the ST6Gal I activity in healthy, transitional and tumour tissues from 43 patients with CRC. By immunohistochemistry we assessed the CDw75 expression in 25 colorectal adenomas, 43 tumours, 13 transitional and 28 healthy tissues of CRC patients.

Results: ST6Gal I activity was likewise found to be statistically higher in tumour tissue respect to healthy tissue from CRC patients. CDw75 expression was positive in 20% of colorectal adenomas. Furthermore, 70% of tumour specimens and 8.3% of transitional specimens were positive for CDw75 expression, whereas none of the healthy ones showed the presence of the epitope.

Conclusion: The major contribution of this study is the inclusion of data from transitional tissue and the analysis of CDw75 antigen expression in CRC and in colorectal adenomas, little known so far. ST6Gal I activity and CDw75 antigen expression were increased in CRC. Although their comparison did not reach the statistical significance, a great extent of patients showed both, an enhanced tumour ST6Gal I activity and an increased CDw75 expression in the tumour tissue. So, these two variables may play a role in malignant transformation. The expression of CDw75 in colorectal adenomas suggests that this antigen may be a tumour marker in CRC.

Show MeSH
Related in: MedlinePlus