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Antioxidant Activity and Lipid-Lowering Effect of Essential Oils Extracted from Ocimum sanctum L. Leaves in Rats Fed with a High Cholesterol Diet.

Suanarunsawat T, Devakul Na Ayutthaya W, Songsak T, Thirawarapan S, Poungshompoo S - J Clin Biochem Nutr (2009)

Bottom Line: In addition, EO was found to decrease the high levels of thiobarbituric acid reactive substances (TBARS), glutathione peroxidase (GPx) and superoxide dismutase (SOD) without impacting catalase (CAT) in the cardiac tissue while in the liver, it decreased high level of TBARS without significantly effecting GPx, SOD and CAT.Histopathological results confirmed that EO preserved the myocardial tissue.Eugenol that is contained in EO likely contribute to these pharmacological effects.

View Article: PubMed Central - PubMed

Affiliation: Physiology Unit, Department of Medical Sciences, Faculty of Science, Rangsit University, Pathumtani, 12000 Thailand.

ABSTRACT
It has been reported that Ocimum sanctum L. (OS) leaves decrease serum lipid profile in normal and diabetic animals. No experimental evidences support the anti-hyperlipidemic and antioxidative actions against hypercholesterolemia. Moreover the identity of the specific chemical ingredients in OS leaves responsible for these pharmacological effects are unknown. Since OS leaves are rich in essential oil (EO). Therefore the present study was conducted to investigate the anti-hyperlipidemic and antioxidative activities of EO extracted from OS leaves in rats fed with high cholesterol (HC) diet. EO was extracted by the hydrodistillation method and the chemical constituents were then identified by Gas Chromatography-Mass Spectrometry. The experiment was performed in Male Wistar rats fed with 2.5 g%(w/w) of cholesterol diet for seven weeks. During the last 3 weeks, rats were daily fed with EO. The results showed that phenyl propanoid compounds including eugenol and methyl eugenol were the major constituents of EO. EO suppressed the high serum lipid profile and atherogenic index as well as serum lactate dehydrogenase and creatine kinase MB subunit without significant effect on high serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in rats fed with HC diet. In addition, EO was found to decrease the high levels of thiobarbituric acid reactive substances (TBARS), glutathione peroxidase (GPx) and superoxide dismutase (SOD) without impacting catalase (CAT) in the cardiac tissue while in the liver, it decreased high level of TBARS without significantly effecting GPx, SOD and CAT. Histopathological results confirmed that EO preserved the myocardial tissue. It can be concluded that EO extracted from OS leaves has lipid-lowering and antioxidative effects that protect the heart against hypercholesterolemia. Eugenol that is contained in EO likely contribute to these pharmacological effects.

No MeSH data available.


Related in: MedlinePlus

Chromatogram of the essential oils extracted from OS leaves
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Related In: Results  -  Collection


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Figure 1: Chromatogram of the essential oils extracted from OS leaves

Mentions: Fig. 1 shows GC of the EO extracted from OS leaves. The chemical constituents of EO are displayed in Table 1. The EO is predominantly composed mainly of phenylpropanoids (65.31%) eugenol and methyl eugenol. 9-epi (E)-Caryophyllene which is sesquiterpene compound, was found 23.68% in EO. Small amounts of monoterpene and oxygenated monoterpene were also found in EO.


Antioxidant Activity and Lipid-Lowering Effect of Essential Oils Extracted from Ocimum sanctum L. Leaves in Rats Fed with a High Cholesterol Diet.

Suanarunsawat T, Devakul Na Ayutthaya W, Songsak T, Thirawarapan S, Poungshompoo S - J Clin Biochem Nutr (2009)

Chromatogram of the essential oils extracted from OS leaves
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2803133&req=5

Figure 1: Chromatogram of the essential oils extracted from OS leaves
Mentions: Fig. 1 shows GC of the EO extracted from OS leaves. The chemical constituents of EO are displayed in Table 1. The EO is predominantly composed mainly of phenylpropanoids (65.31%) eugenol and methyl eugenol. 9-epi (E)-Caryophyllene which is sesquiterpene compound, was found 23.68% in EO. Small amounts of monoterpene and oxygenated monoterpene were also found in EO.

Bottom Line: In addition, EO was found to decrease the high levels of thiobarbituric acid reactive substances (TBARS), glutathione peroxidase (GPx) and superoxide dismutase (SOD) without impacting catalase (CAT) in the cardiac tissue while in the liver, it decreased high level of TBARS without significantly effecting GPx, SOD and CAT.Histopathological results confirmed that EO preserved the myocardial tissue.Eugenol that is contained in EO likely contribute to these pharmacological effects.

View Article: PubMed Central - PubMed

Affiliation: Physiology Unit, Department of Medical Sciences, Faculty of Science, Rangsit University, Pathumtani, 12000 Thailand.

ABSTRACT
It has been reported that Ocimum sanctum L. (OS) leaves decrease serum lipid profile in normal and diabetic animals. No experimental evidences support the anti-hyperlipidemic and antioxidative actions against hypercholesterolemia. Moreover the identity of the specific chemical ingredients in OS leaves responsible for these pharmacological effects are unknown. Since OS leaves are rich in essential oil (EO). Therefore the present study was conducted to investigate the anti-hyperlipidemic and antioxidative activities of EO extracted from OS leaves in rats fed with high cholesterol (HC) diet. EO was extracted by the hydrodistillation method and the chemical constituents were then identified by Gas Chromatography-Mass Spectrometry. The experiment was performed in Male Wistar rats fed with 2.5 g%(w/w) of cholesterol diet for seven weeks. During the last 3 weeks, rats were daily fed with EO. The results showed that phenyl propanoid compounds including eugenol and methyl eugenol were the major constituents of EO. EO suppressed the high serum lipid profile and atherogenic index as well as serum lactate dehydrogenase and creatine kinase MB subunit without significant effect on high serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in rats fed with HC diet. In addition, EO was found to decrease the high levels of thiobarbituric acid reactive substances (TBARS), glutathione peroxidase (GPx) and superoxide dismutase (SOD) without impacting catalase (CAT) in the cardiac tissue while in the liver, it decreased high level of TBARS without significantly effecting GPx, SOD and CAT. Histopathological results confirmed that EO preserved the myocardial tissue. It can be concluded that EO extracted from OS leaves has lipid-lowering and antioxidative effects that protect the heart against hypercholesterolemia. Eugenol that is contained in EO likely contribute to these pharmacological effects.

No MeSH data available.


Related in: MedlinePlus