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Zinc Supplementation with Polaprezinc Protects Mouse Hepatocytes against Acetaminophen-Induced Toxicity via Induction of Heat Shock Protein 70.

Nishida T, Ohata S, Kusumoto C, Mochida S, Nakada J, Inagaki Y, Ohta Y, Matsura T - J Clin Biochem Nutr (2009)

Bottom Line: Pretreatment of the cells with polaprezinc or zinc sulfate significantly suppressed cell death as well as cellular lipid peroxidation after APAP treatment.In contrast, pretreatment with polaprezinc did not affect decrease in intracellular glutathione after APAP.Furthermore, treatment with KNK437, an HSP inhibitor, attenuated increase in HSP70 expression induced by polaprezinc, and abolished protective effect of polaprezinc on cell death after APAP.

View Article: PubMed Central - PubMed

Affiliation: Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.

ABSTRACT
Polaprezinc, a chelate compound consisting of zinc and l-carnosine, is clinically used as a medicine for gastric ulcers. It has been shown that induction of heat shock protein (HSP) is involved in protective effects of polaprezinc against gastric mucosal injury. In the present study, we investigated whether polaprezinc and its components could induce HSP70 and prevent acetaminophen (APAP) toxicity in mouse primary cultured hepatocytes. Hepatocytes were treated with polaprezinc, zinc sulfate or l-carnosine at the concentration of 100 microM for 9 h, and then exposed to 10 mM APAP. Polaprezinc or zinc sulfate increased cellular HSP70 expression. However, l-carnosine had no influence on it. Pretreatment of the cells with polaprezinc or zinc sulfate significantly suppressed cell death as well as cellular lipid peroxidation after APAP treatment. In contrast, pretreatment with polaprezinc did not affect decrease in intracellular glutathione after APAP. Furthermore, treatment with KNK437, an HSP inhibitor, attenuated increase in HSP70 expression induced by polaprezinc, and abolished protective effect of polaprezinc on cell death after APAP. These results suggested that polaprezinc, in particular its zinc component, induces HSP70 expression in mouse primary cultured hepatocytes, and inhibits lipid peroxidation after APAP treatment, resulting in protection against APAP toxicity.

No MeSH data available.


Related in: MedlinePlus

Changes in zinc concentrations after polaprezinc or zinc sulfate treatment. Cells were incubated in the presence of polaprezinc or zinc sulfate at a concentration of 100 µM for 0, 1, 3, 6 or 9 h. Zinc concentration was measured by atomic absorption spectrophotometry. The data are expressed as means ± S.E. of 5 separate experiments. PZ; polaprezinc, ZS; zinc sulfate. **p<0.01, *p<0.05 vs initial (0 h) time point of PZ treatment, ##p<0.01 vs initial (0 h) time point of ZS treatment.
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Figure 2: Changes in zinc concentrations after polaprezinc or zinc sulfate treatment. Cells were incubated in the presence of polaprezinc or zinc sulfate at a concentration of 100 µM for 0, 1, 3, 6 or 9 h. Zinc concentration was measured by atomic absorption spectrophotometry. The data are expressed as means ± S.E. of 5 separate experiments. PZ; polaprezinc, ZS; zinc sulfate. **p<0.01, *p<0.05 vs initial (0 h) time point of PZ treatment, ##p<0.01 vs initial (0 h) time point of ZS treatment.

Mentions: Since zinc reportedly induced HSPs [32, 33], we evaluated zinc concentrations in mouse hepatocytes after polaprezinc or zinc sulfate treatment. The intracellular zinc concentrations increased after treatment with each reagent, reached a peak level after 1 h (1.5- and 1.7-fold of control level, respectively) and remained unchanged from 1 h onwards (Fig. 2).


Zinc Supplementation with Polaprezinc Protects Mouse Hepatocytes against Acetaminophen-Induced Toxicity via Induction of Heat Shock Protein 70.

Nishida T, Ohata S, Kusumoto C, Mochida S, Nakada J, Inagaki Y, Ohta Y, Matsura T - J Clin Biochem Nutr (2009)

Changes in zinc concentrations after polaprezinc or zinc sulfate treatment. Cells were incubated in the presence of polaprezinc or zinc sulfate at a concentration of 100 µM for 0, 1, 3, 6 or 9 h. Zinc concentration was measured by atomic absorption spectrophotometry. The data are expressed as means ± S.E. of 5 separate experiments. PZ; polaprezinc, ZS; zinc sulfate. **p<0.01, *p<0.05 vs initial (0 h) time point of PZ treatment, ##p<0.01 vs initial (0 h) time point of ZS treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2803132&req=5

Figure 2: Changes in zinc concentrations after polaprezinc or zinc sulfate treatment. Cells were incubated in the presence of polaprezinc or zinc sulfate at a concentration of 100 µM for 0, 1, 3, 6 or 9 h. Zinc concentration was measured by atomic absorption spectrophotometry. The data are expressed as means ± S.E. of 5 separate experiments. PZ; polaprezinc, ZS; zinc sulfate. **p<0.01, *p<0.05 vs initial (0 h) time point of PZ treatment, ##p<0.01 vs initial (0 h) time point of ZS treatment.
Mentions: Since zinc reportedly induced HSPs [32, 33], we evaluated zinc concentrations in mouse hepatocytes after polaprezinc or zinc sulfate treatment. The intracellular zinc concentrations increased after treatment with each reagent, reached a peak level after 1 h (1.5- and 1.7-fold of control level, respectively) and remained unchanged from 1 h onwards (Fig. 2).

Bottom Line: Pretreatment of the cells with polaprezinc or zinc sulfate significantly suppressed cell death as well as cellular lipid peroxidation after APAP treatment.In contrast, pretreatment with polaprezinc did not affect decrease in intracellular glutathione after APAP.Furthermore, treatment with KNK437, an HSP inhibitor, attenuated increase in HSP70 expression induced by polaprezinc, and abolished protective effect of polaprezinc on cell death after APAP.

View Article: PubMed Central - PubMed

Affiliation: Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.

ABSTRACT
Polaprezinc, a chelate compound consisting of zinc and l-carnosine, is clinically used as a medicine for gastric ulcers. It has been shown that induction of heat shock protein (HSP) is involved in protective effects of polaprezinc against gastric mucosal injury. In the present study, we investigated whether polaprezinc and its components could induce HSP70 and prevent acetaminophen (APAP) toxicity in mouse primary cultured hepatocytes. Hepatocytes were treated with polaprezinc, zinc sulfate or l-carnosine at the concentration of 100 microM for 9 h, and then exposed to 10 mM APAP. Polaprezinc or zinc sulfate increased cellular HSP70 expression. However, l-carnosine had no influence on it. Pretreatment of the cells with polaprezinc or zinc sulfate significantly suppressed cell death as well as cellular lipid peroxidation after APAP treatment. In contrast, pretreatment with polaprezinc did not affect decrease in intracellular glutathione after APAP. Furthermore, treatment with KNK437, an HSP inhibitor, attenuated increase in HSP70 expression induced by polaprezinc, and abolished protective effect of polaprezinc on cell death after APAP. These results suggested that polaprezinc, in particular its zinc component, induces HSP70 expression in mouse primary cultured hepatocytes, and inhibits lipid peroxidation after APAP treatment, resulting in protection against APAP toxicity.

No MeSH data available.


Related in: MedlinePlus