Limits...
Ginsenoside Rg3 Suppresses Palmitate-Induced Apoptosis in MIN6N8 Pancreatic beta-Cells.

Kim K, Park M, Young Kim H - J Clin Biochem Nutr (2009)

Bottom Line: Ginsenoside Rg3 (Rg3), one of the active ingredients of ginseng saponins, has not been known about the effects on beta-cell apoptosis mediated with FFA.The aims of this study were to investigate the in vitro protective effects of Rg3 on MIN6N8 mouse insulinoma beta-cells against FFA-induced apoptosis, as well as the modulating effects on p44/42 MAPK activation.Our results showed that Rg3 inhibited the palmitate-induced apoptosis through modulating p44/42 MAPK activation.

View Article: PubMed Central - PubMed

Affiliation: Functional Food Technology Research Group, Research Division for Emerging Innovative Techology, Korea Food Research Institute, 516 Baekhyun-dong, Bundang-gu, Songnam-si, Kyonggi-do 463-746, Republic of Korea.

ABSTRACT
Chronic exposure to elevated levels of free fatty acids (FFA) causes beta-cell dysfunction and may induce beta-cell apoptosis in type 2 diabetes. The execution of beta-cell apoptosis occurs through activation of mitogen-activated protein kinases (MAPKs). Ginsenoside Rg3 (Rg3), one of the active ingredients of ginseng saponins, has not been known about the effects on beta-cell apoptosis mediated with FFA. The aims of this study were to investigate the in vitro protective effects of Rg3 on MIN6N8 mouse insulinoma beta-cells against FFA-induced apoptosis, as well as the modulating effects on p44/42 MAPK activation. Our results showed that Rg3 inhibited the palmitate-induced apoptosis through modulating p44/42 MAPK activation. We conclude that Rg3 has the potential role in suppressing the progression of type 2 diabetes by inhibiting FFA-mediated loss of beta-cells.

No MeSH data available.


Related in: MedlinePlus

(A) Effect of ginsenoside Rg3 on cell viability in palmitate-treated MIN6N8 cells. The cells were treated with 500 µM palmitate in the presence or absence of 1–5 µM Rg3 for 48 h. Cell viability was determined by MTT assay. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05). (B) Effect of Rg3 on palmitate-induced apoptosis in MIN6N8 cells. The cells were treated with Rg3 simultaneously with palmitate for 48 h. Apoptosis was determined as the amount of cytosolic histone-associated DNA fragments. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2803130&req=5

Figure 1: (A) Effect of ginsenoside Rg3 on cell viability in palmitate-treated MIN6N8 cells. The cells were treated with 500 µM palmitate in the presence or absence of 1–5 µM Rg3 for 48 h. Cell viability was determined by MTT assay. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05). (B) Effect of Rg3 on palmitate-induced apoptosis in MIN6N8 cells. The cells were treated with Rg3 simultaneously with palmitate for 48 h. Apoptosis was determined as the amount of cytosolic histone-associated DNA fragments. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05).

Mentions: We first examined the effects of Rg3 on the cell viability of MIN6N8 cells using the MTT assay. Cells treated with 500 µM palmitate for 48 h reduced cell viability by approximately 50% relative to the palmitate-untreated cells. This cytotoxic effect was attenuated by co-treatment with Rg3 in 1–5 µM in part dose-dependently (Fig. 1A). Palmitate-untreated cells administered with 1–5 µM Rg3 showed no difference on cell viability from that of the palmitate-untreated control cells (not shown in data).


Ginsenoside Rg3 Suppresses Palmitate-Induced Apoptosis in MIN6N8 Pancreatic beta-Cells.

Kim K, Park M, Young Kim H - J Clin Biochem Nutr (2009)

(A) Effect of ginsenoside Rg3 on cell viability in palmitate-treated MIN6N8 cells. The cells were treated with 500 µM palmitate in the presence or absence of 1–5 µM Rg3 for 48 h. Cell viability was determined by MTT assay. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05). (B) Effect of Rg3 on palmitate-induced apoptosis in MIN6N8 cells. The cells were treated with Rg3 simultaneously with palmitate for 48 h. Apoptosis was determined as the amount of cytosolic histone-associated DNA fragments. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2803130&req=5

Figure 1: (A) Effect of ginsenoside Rg3 on cell viability in palmitate-treated MIN6N8 cells. The cells were treated with 500 µM palmitate in the presence or absence of 1–5 µM Rg3 for 48 h. Cell viability was determined by MTT assay. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05). (B) Effect of Rg3 on palmitate-induced apoptosis in MIN6N8 cells. The cells were treated with Rg3 simultaneously with palmitate for 48 h. Apoptosis was determined as the amount of cytosolic histone-associated DNA fragments. Values are means ± SD (n = 9). Means with different letters differ significantly among groups (p<0.05).
Mentions: We first examined the effects of Rg3 on the cell viability of MIN6N8 cells using the MTT assay. Cells treated with 500 µM palmitate for 48 h reduced cell viability by approximately 50% relative to the palmitate-untreated cells. This cytotoxic effect was attenuated by co-treatment with Rg3 in 1–5 µM in part dose-dependently (Fig. 1A). Palmitate-untreated cells administered with 1–5 µM Rg3 showed no difference on cell viability from that of the palmitate-untreated control cells (not shown in data).

Bottom Line: Ginsenoside Rg3 (Rg3), one of the active ingredients of ginseng saponins, has not been known about the effects on beta-cell apoptosis mediated with FFA.The aims of this study were to investigate the in vitro protective effects of Rg3 on MIN6N8 mouse insulinoma beta-cells against FFA-induced apoptosis, as well as the modulating effects on p44/42 MAPK activation.Our results showed that Rg3 inhibited the palmitate-induced apoptosis through modulating p44/42 MAPK activation.

View Article: PubMed Central - PubMed

Affiliation: Functional Food Technology Research Group, Research Division for Emerging Innovative Techology, Korea Food Research Institute, 516 Baekhyun-dong, Bundang-gu, Songnam-si, Kyonggi-do 463-746, Republic of Korea.

ABSTRACT
Chronic exposure to elevated levels of free fatty acids (FFA) causes beta-cell dysfunction and may induce beta-cell apoptosis in type 2 diabetes. The execution of beta-cell apoptosis occurs through activation of mitogen-activated protein kinases (MAPKs). Ginsenoside Rg3 (Rg3), one of the active ingredients of ginseng saponins, has not been known about the effects on beta-cell apoptosis mediated with FFA. The aims of this study were to investigate the in vitro protective effects of Rg3 on MIN6N8 mouse insulinoma beta-cells against FFA-induced apoptosis, as well as the modulating effects on p44/42 MAPK activation. Our results showed that Rg3 inhibited the palmitate-induced apoptosis through modulating p44/42 MAPK activation. We conclude that Rg3 has the potential role in suppressing the progression of type 2 diabetes by inhibiting FFA-mediated loss of beta-cells.

No MeSH data available.


Related in: MedlinePlus