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An agarose-gel based method for transporting cell lines.

Yang L, Li C, Chen L, Li Z - Curr Chem Genomics (2009)

Bottom Line: Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability.Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process.This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences (GIBH), Guangzhou 510663, China.

ABSTRACT
Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process. We have recently developed an agarose gel based method for directly transporting the live adherent cells in cell culture plates or dishes in ambient temperature. This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.

No MeSH data available.


Related in: MedlinePlus

The Effect of agarose concentration on colony-formation.
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Figure 3: The Effect of agarose concentration on colony-formation.

Mentions: We used this colony formation experiment to test the viability and function of the agarose-medium gel preserved cells after transportation. In this colony formation experiment, cells are sparsely seeded in 6 cm dish (e.g. 500 cells/dish) and cultured for 7 to 10 days for the colonies to grow. The numbers of colony formation in the recovered cells after being kept 1 to 3 days in the agarose-medium gel at room temperature were similar to these in regular cell culture condition. The results of colony formation experiment showed that the morphology and size of cell colonies for three cell lines were remained similar after recovering from the transportation with the agarose-medium gel method (Fig. 3). We observed that the number of colonies was reduced in the HEK293 and A549 cell lines after the 1.2% agarose treatment though these remained unchanged at the lower agarose concentration (Fig. 3). This result suggested that the agarose concentration higher than 1.2 % might may reduce the cell viability with this transportation method. Thus, agarose concentration at 1 % is an optimal condition for this cell transportation method because it has the highest cell recovery viability and is firm enough to form a proper layer of gel to protect cell during the transportation.


An agarose-gel based method for transporting cell lines.

Yang L, Li C, Chen L, Li Z - Curr Chem Genomics (2009)

The Effect of agarose concentration on colony-formation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2802760&req=5

Figure 3: The Effect of agarose concentration on colony-formation.
Mentions: We used this colony formation experiment to test the viability and function of the agarose-medium gel preserved cells after transportation. In this colony formation experiment, cells are sparsely seeded in 6 cm dish (e.g. 500 cells/dish) and cultured for 7 to 10 days for the colonies to grow. The numbers of colony formation in the recovered cells after being kept 1 to 3 days in the agarose-medium gel at room temperature were similar to these in regular cell culture condition. The results of colony formation experiment showed that the morphology and size of cell colonies for three cell lines were remained similar after recovering from the transportation with the agarose-medium gel method (Fig. 3). We observed that the number of colonies was reduced in the HEK293 and A549 cell lines after the 1.2% agarose treatment though these remained unchanged at the lower agarose concentration (Fig. 3). This result suggested that the agarose concentration higher than 1.2 % might may reduce the cell viability with this transportation method. Thus, agarose concentration at 1 % is an optimal condition for this cell transportation method because it has the highest cell recovery viability and is firm enough to form a proper layer of gel to protect cell during the transportation.

Bottom Line: Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability.Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process.This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences (GIBH), Guangzhou 510663, China.

ABSTRACT
Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process. We have recently developed an agarose gel based method for directly transporting the live adherent cells in cell culture plates or dishes in ambient temperature. This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.

No MeSH data available.


Related in: MedlinePlus