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Ophiostoma denticiliatum sp. nov. and other Ophiostoma species associated with the birch bark beetle in southern Norway.

Linnakoski R, de Beer ZW, Rousi M, Solheim H, Wingfield MJ - Persoonia (2009)

Bottom Line: The most abundant fungus was Ophiostoma karelicum, suggesting a specific relationship between the fungus, the vector insect and the host tree.Our results suggest that O. karelicum occurs across the geographic range of S. ratzeburgi and its close relatedness to the Dutch elm disease fungi suggests that it could be important if introduced into other parts of the world.Other fungi, only occasionally isolated from S. ratzeburgi, were identified as O. quercus and a novel taxon, described here as O. denticiliatum sp. nov.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Forest Sciences, University of Joensuu, P.O. Box 111, FI-80101 Joensuu, Finland;

ABSTRACT
Ophiostomatoid fungi were isolated from Scolytus ratzeburgi infesting Betula pendula and B. pubescens in Norway. Fungi were identified based on morphology, DNA sequence comparison for two gene regions and phylogenetic analyses. The most abundant fungus was Ophiostoma karelicum, suggesting a specific relationship between the fungus, the vector insect and the host tree. Our results suggest that O. karelicum occurs across the geographic range of S. ratzeburgi and its close relatedness to the Dutch elm disease fungi suggests that it could be important if introduced into other parts of the world. Other fungi, only occasionally isolated from S. ratzeburgi, were identified as O. quercus and a novel taxon, described here as O. denticiliatum sp. nov.

No MeSH data available.


Phylograms obtained from Neighbour-joining analyses of DNA sequences of (a) the nuclear ITS region and (b–d) the β-tubulin (BT) gene. Bootstrap support values (1 000 replicates) above 75 % are indicated at the nodes (normal type for Neighbour-joining, bold type for maximum parsimony). Posterior probabilities (above 90 %) obtained from Bayesian analyses are indicated by bold lines at the relevant branching points. * = bootstrap values lower than 75 %. Isolate numbers of sequences obtained in this study are printed in bold type. — Scale bar = total nucleotide difference between taxa.
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Figure 1: Phylograms obtained from Neighbour-joining analyses of DNA sequences of (a) the nuclear ITS region and (b–d) the β-tubulin (BT) gene. Bootstrap support values (1 000 replicates) above 75 % are indicated at the nodes (normal type for Neighbour-joining, bold type for maximum parsimony). Posterior probabilities (above 90 %) obtained from Bayesian analyses are indicated by bold lines at the relevant branching points. * = bootstrap values lower than 75 %. Isolate numbers of sequences obtained in this study are printed in bold type. — Scale bar = total nucleotide difference between taxa.

Mentions: Aligned DNA sequence from the ITS regions yielded 715 characters including gaps (Fig. 1a), while alignments of the three β-tubulin data subsets consisted of 339, 332 and 329 characters, respectively, including gaps (Fig. 1b–d). All the trees presented were obtained from NJ analyses (Fig. 1). The Consistency Indices (CI) obtained from heuristic searches in MP analyses for the four datasets were 0.757, 0.819, 0.886 and 0.933, respectively, while the Retention Indices were 0.926, 0.889, 0.925 and 0.941, respectively. The Bayesian analyses for ITS and β-tubulin gene regions produced trees with topologies similar to those of the NJ and MP analyses. The best fitting substitution model selected for the Bayesian analyses was GTR+I+G for all the datasets.


Ophiostoma denticiliatum sp. nov. and other Ophiostoma species associated with the birch bark beetle in southern Norway.

Linnakoski R, de Beer ZW, Rousi M, Solheim H, Wingfield MJ - Persoonia (2009)

Phylograms obtained from Neighbour-joining analyses of DNA sequences of (a) the nuclear ITS region and (b–d) the β-tubulin (BT) gene. Bootstrap support values (1 000 replicates) above 75 % are indicated at the nodes (normal type for Neighbour-joining, bold type for maximum parsimony). Posterior probabilities (above 90 %) obtained from Bayesian analyses are indicated by bold lines at the relevant branching points. * = bootstrap values lower than 75 %. Isolate numbers of sequences obtained in this study are printed in bold type. — Scale bar = total nucleotide difference between taxa.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2802728&req=5

Figure 1: Phylograms obtained from Neighbour-joining analyses of DNA sequences of (a) the nuclear ITS region and (b–d) the β-tubulin (BT) gene. Bootstrap support values (1 000 replicates) above 75 % are indicated at the nodes (normal type for Neighbour-joining, bold type for maximum parsimony). Posterior probabilities (above 90 %) obtained from Bayesian analyses are indicated by bold lines at the relevant branching points. * = bootstrap values lower than 75 %. Isolate numbers of sequences obtained in this study are printed in bold type. — Scale bar = total nucleotide difference between taxa.
Mentions: Aligned DNA sequence from the ITS regions yielded 715 characters including gaps (Fig. 1a), while alignments of the three β-tubulin data subsets consisted of 339, 332 and 329 characters, respectively, including gaps (Fig. 1b–d). All the trees presented were obtained from NJ analyses (Fig. 1). The Consistency Indices (CI) obtained from heuristic searches in MP analyses for the four datasets were 0.757, 0.819, 0.886 and 0.933, respectively, while the Retention Indices were 0.926, 0.889, 0.925 and 0.941, respectively. The Bayesian analyses for ITS and β-tubulin gene regions produced trees with topologies similar to those of the NJ and MP analyses. The best fitting substitution model selected for the Bayesian analyses was GTR+I+G for all the datasets.

Bottom Line: The most abundant fungus was Ophiostoma karelicum, suggesting a specific relationship between the fungus, the vector insect and the host tree.Our results suggest that O. karelicum occurs across the geographic range of S. ratzeburgi and its close relatedness to the Dutch elm disease fungi suggests that it could be important if introduced into other parts of the world.Other fungi, only occasionally isolated from S. ratzeburgi, were identified as O. quercus and a novel taxon, described here as O. denticiliatum sp. nov.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Forest Sciences, University of Joensuu, P.O. Box 111, FI-80101 Joensuu, Finland;

ABSTRACT
Ophiostomatoid fungi were isolated from Scolytus ratzeburgi infesting Betula pendula and B. pubescens in Norway. Fungi were identified based on morphology, DNA sequence comparison for two gene regions and phylogenetic analyses. The most abundant fungus was Ophiostoma karelicum, suggesting a specific relationship between the fungus, the vector insect and the host tree. Our results suggest that O. karelicum occurs across the geographic range of S. ratzeburgi and its close relatedness to the Dutch elm disease fungi suggests that it could be important if introduced into other parts of the world. Other fungi, only occasionally isolated from S. ratzeburgi, were identified as O. quercus and a novel taxon, described here as O. denticiliatum sp. nov.

No MeSH data available.