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Barriopsis iraniana and Phaeobotryon cupressi: two new species of the Botryosphaeriaceae from trees in Iran.

Abdollahzadeh J, Mohammadi Goltapeh E, Javadi A, Shams-Bakhsh M, Zare R, Phillips AJ - Persoonia (2009)

Bottom Line: In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts.Phylogenetic analyses placed them within a clade consisting of Barriopsis and Phaeobotryon species, but they were clearly distinct from known species in these genera.Therefore, they are described here as two new species, namely Barriopsis iraniana on Citrus, Mangifera and Olea, and Phaeobotryon cupressi on Cupressus sempervirens.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.

ABSTRACT
Species in the Botryosphaeriaceae are well known as pathogens and saprobes of woody hosts, but little is known about the species that occur in Iran. In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts. These two fungi were fully characterised in terms of morphology of the anamorphs in culture, and sequences of the ITS1/ITS2 regions of the ribosomal DNA operon and partial sequences of the translation elongation factor 1-alpha. Phylogenetic analyses placed them within a clade consisting of Barriopsis and Phaeobotryon species, but they were clearly distinct from known species in these genera. Therefore, they are described here as two new species, namely Barriopsis iraniana on Citrus, Mangifera and Olea, and Phaeobotryon cupressi on Cupressus sempervirens.

No MeSH data available.


Single most parsimonious tree resulting from maximum parsimony analysis of combined ITS and EF1-α sequence data. MP bootstrap values are given at the nodes. The tree was rooted to two isolates of Pseudofusicoccum stromaticum. The new species are in bold face.
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Figure 1: Single most parsimonious tree resulting from maximum parsimony analysis of combined ITS and EF1-α sequence data. MP bootstrap values are given at the nodes. The tree was rooted to two isolates of Pseudofusicoccum stromaticum. The new species are in bold face.

Mentions: The ITS and EF1-α sequences for the 11 isolates studied were combined and aligned with 41 sequences of 22 taxa retrieved from GenBank, representing a selection of genera and species in the Botryosphaeriaceae. Incomplete portions at the ends of the sequences were excluded from the analyses. The combined dataset after alignment consisted of 941 characters including alignment gaps. A partition homogeneity test in PAUP was not significant (P = 0.52) indicating that the individual datasets were congruent and produced trees with the same topology. Therefore the two datasets were combined in a single analysis. Of the 941 characters, 446 were constant, while 10 were variable and parsimony-uninformative. Maximum parsimony analysis of the remaining 485 parsimony-informative characters resulted in a single tree of 1 340 steps (HI = 0.343). The overall topology of the 50 % majority rule consensus tree of 9 000 trees sampled in the Bayesian analysis had a similar topology as the MP tree (TreeBase S2392), which is presented in Fig. 1.


Barriopsis iraniana and Phaeobotryon cupressi: two new species of the Botryosphaeriaceae from trees in Iran.

Abdollahzadeh J, Mohammadi Goltapeh E, Javadi A, Shams-Bakhsh M, Zare R, Phillips AJ - Persoonia (2009)

Single most parsimonious tree resulting from maximum parsimony analysis of combined ITS and EF1-α sequence data. MP bootstrap values are given at the nodes. The tree was rooted to two isolates of Pseudofusicoccum stromaticum. The new species are in bold face.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2802722&req=5

Figure 1: Single most parsimonious tree resulting from maximum parsimony analysis of combined ITS and EF1-α sequence data. MP bootstrap values are given at the nodes. The tree was rooted to two isolates of Pseudofusicoccum stromaticum. The new species are in bold face.
Mentions: The ITS and EF1-α sequences for the 11 isolates studied were combined and aligned with 41 sequences of 22 taxa retrieved from GenBank, representing a selection of genera and species in the Botryosphaeriaceae. Incomplete portions at the ends of the sequences were excluded from the analyses. The combined dataset after alignment consisted of 941 characters including alignment gaps. A partition homogeneity test in PAUP was not significant (P = 0.52) indicating that the individual datasets were congruent and produced trees with the same topology. Therefore the two datasets were combined in a single analysis. Of the 941 characters, 446 were constant, while 10 were variable and parsimony-uninformative. Maximum parsimony analysis of the remaining 485 parsimony-informative characters resulted in a single tree of 1 340 steps (HI = 0.343). The overall topology of the 50 % majority rule consensus tree of 9 000 trees sampled in the Bayesian analysis had a similar topology as the MP tree (TreeBase S2392), which is presented in Fig. 1.

Bottom Line: In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts.Phylogenetic analyses placed them within a clade consisting of Barriopsis and Phaeobotryon species, but they were clearly distinct from known species in these genera.Therefore, they are described here as two new species, namely Barriopsis iraniana on Citrus, Mangifera and Olea, and Phaeobotryon cupressi on Cupressus sempervirens.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.

ABSTRACT
Species in the Botryosphaeriaceae are well known as pathogens and saprobes of woody hosts, but little is known about the species that occur in Iran. In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts. These two fungi were fully characterised in terms of morphology of the anamorphs in culture, and sequences of the ITS1/ITS2 regions of the ribosomal DNA operon and partial sequences of the translation elongation factor 1-alpha. Phylogenetic analyses placed them within a clade consisting of Barriopsis and Phaeobotryon species, but they were clearly distinct from known species in these genera. Therefore, they are described here as two new species, namely Barriopsis iraniana on Citrus, Mangifera and Olea, and Phaeobotryon cupressi on Cupressus sempervirens.

No MeSH data available.