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Improvement of trochanteric bone quality in an osteoporosis model after short-term treatment with parathyroid hormone: a new mechanical test for trochanteric region of rat femur.

Tezval M, Stuermer EK, Sehmisch S, Rack T, Stary A, Stebener M, Konietschke F, Stuermer KM - Osteoporos Int (2009)

Bottom Line: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

View Article: PubMed Central - PubMed

Affiliation: Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany. mtezval@med.uni-goettingen.de

ABSTRACT

Unlabelled: We have examined the changes induced in the trochanteric region of femur of ovariectomized rat after administration of estradiol and parathyroid hormone. We have developed a reproducible biomechanical test and produced trochanteric fractures to evaluate stiffness and strength of this region in addition to histomorphometry.

Introduction: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.

Methods: Forty-four 3-month-old female Sprague-Dawley rats were ovariectomized and 8 weeks later treated with soy-free diet (C), daily applications of orally supplied E (0.5 mg/kg food) or subcutaneously injected PTH (0.014 mg/kg), for 5 weeks, and an additional untreated group was added as sham-operated. The femurs were examined for biomechanical and histomorphometric changes.

Results: Our new mechanical test was validated in a right-left comparison. The PTH treatment induced significantly superior biomechanical results (F (max) = 225.3 N, stiffness = 314.9 N/mm) compared to E (F (max) = 182.9 N, stiffness = 237.2 N/mm), C (F (max) = 166.03 N, stiffness = 235.56 N/mm), and sham (F (max) = 192.1 N, stiffness = 267.2 N/mm). Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.

Conclusion: Our new biomechanical test is valid and produces trochanteric fracture. Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

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Microradiographs of the proximal femur of rat show the high content of both cortical and trabecular surfaces (after breaking test). a The control group (C) with a low trabecular density. b The rat femur after treatment with estradiol, c the sham group, and d after substitution with PTH (high trabecular content and thick cortical shell)
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Fig5: Microradiographs of the proximal femur of rat show the high content of both cortical and trabecular surfaces (after breaking test). a The control group (C) with a low trabecular density. b The rat femur after treatment with estradiol, c the sham group, and d after substitution with PTH (high trabecular content and thick cortical shell)

Mentions: The epiphysis line of the capitis femoris represented the proximal border of the histomorphometry frame. The distal limit of the sections was marked by the base of the major trochanter (Fig. 5a–d). We assessed ratio of trabecular bone area to total cancellous bone area at the prox. Femur (Tb.Ar) and trabecular connectivity (N. Nd/mm2) and trabecular thickness (Tb.Wi).


Improvement of trochanteric bone quality in an osteoporosis model after short-term treatment with parathyroid hormone: a new mechanical test for trochanteric region of rat femur.

Tezval M, Stuermer EK, Sehmisch S, Rack T, Stary A, Stebener M, Konietschke F, Stuermer KM - Osteoporos Int (2009)

Microradiographs of the proximal femur of rat show the high content of both cortical and trabecular surfaces (after breaking test). a The control group (C) with a low trabecular density. b The rat femur after treatment with estradiol, c the sham group, and d after substitution with PTH (high trabecular content and thick cortical shell)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801842&req=5

Fig5: Microradiographs of the proximal femur of rat show the high content of both cortical and trabecular surfaces (after breaking test). a The control group (C) with a low trabecular density. b The rat femur after treatment with estradiol, c the sham group, and d after substitution with PTH (high trabecular content and thick cortical shell)
Mentions: The epiphysis line of the capitis femoris represented the proximal border of the histomorphometry frame. The distal limit of the sections was marked by the base of the major trochanter (Fig. 5a–d). We assessed ratio of trabecular bone area to total cancellous bone area at the prox. Femur (Tb.Ar) and trabecular connectivity (N. Nd/mm2) and trabecular thickness (Tb.Wi).

Bottom Line: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

View Article: PubMed Central - PubMed

Affiliation: Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany. mtezval@med.uni-goettingen.de

ABSTRACT

Unlabelled: We have examined the changes induced in the trochanteric region of femur of ovariectomized rat after administration of estradiol and parathyroid hormone. We have developed a reproducible biomechanical test and produced trochanteric fractures to evaluate stiffness and strength of this region in addition to histomorphometry.

Introduction: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.

Methods: Forty-four 3-month-old female Sprague-Dawley rats were ovariectomized and 8 weeks later treated with soy-free diet (C), daily applications of orally supplied E (0.5 mg/kg food) or subcutaneously injected PTH (0.014 mg/kg), for 5 weeks, and an additional untreated group was added as sham-operated. The femurs were examined for biomechanical and histomorphometric changes.

Results: Our new mechanical test was validated in a right-left comparison. The PTH treatment induced significantly superior biomechanical results (F (max) = 225.3 N, stiffness = 314.9 N/mm) compared to E (F (max) = 182.9 N, stiffness = 237.2 N/mm), C (F (max) = 166.03 N, stiffness = 235.56 N/mm), and sham (F (max) = 192.1 N, stiffness = 267.2 N/mm). Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.

Conclusion: Our new biomechanical test is valid and produces trochanteric fracture. Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

Show MeSH
Related in: MedlinePlus