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Improvement of trochanteric bone quality in an osteoporosis model after short-term treatment with parathyroid hormone: a new mechanical test for trochanteric region of rat femur.

Tezval M, Stuermer EK, Sehmisch S, Rack T, Stary A, Stebener M, Konietschke F, Stuermer KM - Osteoporos Int (2009)

Bottom Line: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

View Article: PubMed Central - PubMed

Affiliation: Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany. mtezval@med.uni-goettingen.de

ABSTRACT

Unlabelled: We have examined the changes induced in the trochanteric region of femur of ovariectomized rat after administration of estradiol and parathyroid hormone. We have developed a reproducible biomechanical test and produced trochanteric fractures to evaluate stiffness and strength of this region in addition to histomorphometry.

Introduction: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.

Methods: Forty-four 3-month-old female Sprague-Dawley rats were ovariectomized and 8 weeks later treated with soy-free diet (C), daily applications of orally supplied E (0.5 mg/kg food) or subcutaneously injected PTH (0.014 mg/kg), for 5 weeks, and an additional untreated group was added as sham-operated. The femurs were examined for biomechanical and histomorphometric changes.

Results: Our new mechanical test was validated in a right-left comparison. The PTH treatment induced significantly superior biomechanical results (F (max) = 225.3 N, stiffness = 314.9 N/mm) compared to E (F (max) = 182.9 N, stiffness = 237.2 N/mm), C (F (max) = 166.03 N, stiffness = 235.56 N/mm), and sham (F (max) = 192.1 N, stiffness = 267.2 N/mm). Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.

Conclusion: Our new biomechanical test is valid and produces trochanteric fracture. Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

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Scatter plots from right–left comparison of rat femurs in the new breaking test. The mean difference between the trochanteric loads of both femurs 9.8% for the maximum load (Fmax), 11.5% for the failure load (fL), 21.4% for the stiffness (elasticity), and 9.3% for the yield load
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Fig3: Scatter plots from right–left comparison of rat femurs in the new breaking test. The mean difference between the trochanteric loads of both femurs 9.8% for the maximum load (Fmax), 11.5% for the failure load (fL), 21.4% for the stiffness (elasticity), and 9.3% for the yield load

Mentions: A graphical comparison of the strength of each femur in individual rats showed great similarity. The scatter plots from the results of the right–left comparison are presented in Fig. 3.Fig. 3


Improvement of trochanteric bone quality in an osteoporosis model after short-term treatment with parathyroid hormone: a new mechanical test for trochanteric region of rat femur.

Tezval M, Stuermer EK, Sehmisch S, Rack T, Stary A, Stebener M, Konietschke F, Stuermer KM - Osteoporos Int (2009)

Scatter plots from right–left comparison of rat femurs in the new breaking test. The mean difference between the trochanteric loads of both femurs 9.8% for the maximum load (Fmax), 11.5% for the failure load (fL), 21.4% for the stiffness (elasticity), and 9.3% for the yield load
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801842&req=5

Fig3: Scatter plots from right–left comparison of rat femurs in the new breaking test. The mean difference between the trochanteric loads of both femurs 9.8% for the maximum load (Fmax), 11.5% for the failure load (fL), 21.4% for the stiffness (elasticity), and 9.3% for the yield load
Mentions: A graphical comparison of the strength of each femur in individual rats showed great similarity. The scatter plots from the results of the right–left comparison are presented in Fig. 3.Fig. 3

Bottom Line: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

View Article: PubMed Central - PubMed

Affiliation: Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany. mtezval@med.uni-goettingen.de

ABSTRACT

Unlabelled: We have examined the changes induced in the trochanteric region of femur of ovariectomized rat after administration of estradiol and parathyroid hormone. We have developed a reproducible biomechanical test and produced trochanteric fractures to evaluate stiffness and strength of this region in addition to histomorphometry.

Introduction: We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test.

Methods: Forty-four 3-month-old female Sprague-Dawley rats were ovariectomized and 8 weeks later treated with soy-free diet (C), daily applications of orally supplied E (0.5 mg/kg food) or subcutaneously injected PTH (0.014 mg/kg), for 5 weeks, and an additional untreated group was added as sham-operated. The femurs were examined for biomechanical and histomorphometric changes.

Results: Our new mechanical test was validated in a right-left comparison. The PTH treatment induced significantly superior biomechanical results (F (max) = 225.3 N, stiffness = 314.9 N/mm) compared to E (F (max) = 182.9 N, stiffness = 237.2 N/mm), C (F (max) = 166.03 N, stiffness = 235.56 N/mm), and sham (F (max) = 192.1 N, stiffness = 267.2 N/mm). Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups.

Conclusion: Our new biomechanical test is valid and produces trochanteric fracture. Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E.

Show MeSH
Related in: MedlinePlus