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A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen.

Lee HL, Kim SH, Ji DB, Kim YJ - J. Vet. Sci. (2009)

Bottom Line: The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen.These results indicate that spermatozoa with good quality can be achieved by these three separation techniques and can be used for bovine IVF.In particular, it suggests that glass wool filtration would be the most effective method of the three for improving sperm quality and embryo production for cryopreserved bovine spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Korea.

ABSTRACT
The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The motility of samples obtained by the three separation techniques were greater compared to the control samples (p < 0.05). The percentage of spermatozoa with intact plasma-membrane integrity, identified by 6-carboxyfluoresceindiacetate/ propidium iodide fluorescent staining and the hypo-osmotic swelling test, was highest in the glass wool filtration samples (p < 0.05). The cleavage and blastocyst rate of total oocytes produced from glass wool filtration samples were also higher than the control and Sephadex filtration samples (p < 0.05), but were not significantly different from Percoll separation samples. However, a significantly greater number of cleaved embryos produced by glass wool filtration developed to blastocyst stage than those produced by Percoll separation (p < 0.05). These results indicate that spermatozoa with good quality can be achieved by these three separation techniques and can be used for bovine IVF. In particular, it suggests that glass wool filtration would be the most effective method of the three for improving sperm quality and embryo production for cryopreserved bovine spermatozoa.

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Sperm motility in treated and control samples. Data are presented as mean ± SE. a,bDifferent superscripts indicate significant differences among treatments (p < 0.05, n = 6).
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Figure 1: Sperm motility in treated and control samples. Data are presented as mean ± SE. a,bDifferent superscripts indicate significant differences among treatments (p < 0.05, n = 6).

Mentions: In order to evaluate and to compare the effectiveness of different spermatozoa treatments, the percentage of motile spermatozoa from cryopreserved bovine semen was determined. From the data presented in Fig. 1, spermatozoa recovered by the different spermatozoa treatments showed a significant increase in the percentage of motility with respect to control samples (p < 0.05), but the percentage of motility did not differ among the spermatozoa treatments (p > 0.05). The percentage of motility was 45.83 ± 7.35, 64.17 ± 6.51, 65.83 ± 5.98 and 70.83 ± 6.25% for control, Sephadex filtration, glass wool filtration and Percoll separation samples, respectively. The percentage of spermatozoa with normal morphology was not significantly different among all groups (Table 1) and was above 80% in all groups.


A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen.

Lee HL, Kim SH, Ji DB, Kim YJ - J. Vet. Sci. (2009)

Sperm motility in treated and control samples. Data are presented as mean ± SE. a,bDifferent superscripts indicate significant differences among treatments (p < 0.05, n = 6).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801134&req=5

Figure 1: Sperm motility in treated and control samples. Data are presented as mean ± SE. a,bDifferent superscripts indicate significant differences among treatments (p < 0.05, n = 6).
Mentions: In order to evaluate and to compare the effectiveness of different spermatozoa treatments, the percentage of motile spermatozoa from cryopreserved bovine semen was determined. From the data presented in Fig. 1, spermatozoa recovered by the different spermatozoa treatments showed a significant increase in the percentage of motility with respect to control samples (p < 0.05), but the percentage of motility did not differ among the spermatozoa treatments (p > 0.05). The percentage of motility was 45.83 ± 7.35, 64.17 ± 6.51, 65.83 ± 5.98 and 70.83 ± 6.25% for control, Sephadex filtration, glass wool filtration and Percoll separation samples, respectively. The percentage of spermatozoa with normal morphology was not significantly different among all groups (Table 1) and was above 80% in all groups.

Bottom Line: The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen.These results indicate that spermatozoa with good quality can be achieved by these three separation techniques and can be used for bovine IVF.In particular, it suggests that glass wool filtration would be the most effective method of the three for improving sperm quality and embryo production for cryopreserved bovine spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Korea.

ABSTRACT
The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The motility of samples obtained by the three separation techniques were greater compared to the control samples (p < 0.05). The percentage of spermatozoa with intact plasma-membrane integrity, identified by 6-carboxyfluoresceindiacetate/ propidium iodide fluorescent staining and the hypo-osmotic swelling test, was highest in the glass wool filtration samples (p < 0.05). The cleavage and blastocyst rate of total oocytes produced from glass wool filtration samples were also higher than the control and Sephadex filtration samples (p < 0.05), but were not significantly different from Percoll separation samples. However, a significantly greater number of cleaved embryos produced by glass wool filtration developed to blastocyst stage than those produced by Percoll separation (p < 0.05). These results indicate that spermatozoa with good quality can be achieved by these three separation techniques and can be used for bovine IVF. In particular, it suggests that glass wool filtration would be the most effective method of the three for improving sperm quality and embryo production for cryopreserved bovine spermatozoa.

Show MeSH
Related in: MedlinePlus