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Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine.

Lee J, Moon C, Kim J, Jung C, Lee KH, Joo HG, Ahn M, Shin T - J. Vet. Sci. (2009)

Bottom Line: The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease.Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum.These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.

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Related in: MedlinePlus

Histochemical detection of acid-fast bacteria and immunohistochemical localization of galectin-3 in serial sections of small intestines infected with Johne's disease. Acid-fast bacilli were identified in the lamina propria of the small intestines using Ziehl-Neelsen staining (red color, A and C). Galectin-3 immunoreactivity (B and D) overlapped with acid-fast bacteria-containing cells (A) in the adjacent section. C and D show higher magnifications of arrow indicated fields in A and B, respectively. A and C: Ziehl-Neelsen staining. B and D: Immunostaining of galectin-3. A and B: Scale bars = 200 µm. C and D: Scale bars = 50 µm.
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Figure 2: Histochemical detection of acid-fast bacteria and immunohistochemical localization of galectin-3 in serial sections of small intestines infected with Johne's disease. Acid-fast bacilli were identified in the lamina propria of the small intestines using Ziehl-Neelsen staining (red color, A and C). Galectin-3 immunoreactivity (B and D) overlapped with acid-fast bacteria-containing cells (A) in the adjacent section. C and D show higher magnifications of arrow indicated fields in A and B, respectively. A and C: Ziehl-Neelsen staining. B and D: Immunostaining of galectin-3. A and B: Scale bars = 200 µm. C and D: Scale bars = 50 µm.

Mentions: In the acid-fast stained tissue sections of the small intestine, the presence of acid-fast bacilli was confirmed due to its red coloration (Fig. 2A). Acid-fast bacilli were found in various cell types, including macrophages, in the lamina propria and submucosa of the small intestine. The lamina propria just below the epithelium of the villi was intensely stained red, suggesting that acid-fast bacilli were compacted in macrophages within this area (Fig. 2C). In the adjacent tissues of the acid-fast stained section, galectin-3 was strongly expressed in the round cells (typical of macrophages) in the lamina propria and submucosa of the intestines (Fig. 2B) and moderately expressed in the intestinal epithelium. The intense staining patterns of galectin-3 in the villous lamina propria (Fig. 2D) largely overlapped with the acid-fast staining (Fig. 2C).


Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine.

Lee J, Moon C, Kim J, Jung C, Lee KH, Joo HG, Ahn M, Shin T - J. Vet. Sci. (2009)

Histochemical detection of acid-fast bacteria and immunohistochemical localization of galectin-3 in serial sections of small intestines infected with Johne's disease. Acid-fast bacilli were identified in the lamina propria of the small intestines using Ziehl-Neelsen staining (red color, A and C). Galectin-3 immunoreactivity (B and D) overlapped with acid-fast bacteria-containing cells (A) in the adjacent section. C and D show higher magnifications of arrow indicated fields in A and B, respectively. A and C: Ziehl-Neelsen staining. B and D: Immunostaining of galectin-3. A and B: Scale bars = 200 µm. C and D: Scale bars = 50 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801129&req=5

Figure 2: Histochemical detection of acid-fast bacteria and immunohistochemical localization of galectin-3 in serial sections of small intestines infected with Johne's disease. Acid-fast bacilli were identified in the lamina propria of the small intestines using Ziehl-Neelsen staining (red color, A and C). Galectin-3 immunoreactivity (B and D) overlapped with acid-fast bacteria-containing cells (A) in the adjacent section. C and D show higher magnifications of arrow indicated fields in A and B, respectively. A and C: Ziehl-Neelsen staining. B and D: Immunostaining of galectin-3. A and B: Scale bars = 200 µm. C and D: Scale bars = 50 µm.
Mentions: In the acid-fast stained tissue sections of the small intestine, the presence of acid-fast bacilli was confirmed due to its red coloration (Fig. 2A). Acid-fast bacilli were found in various cell types, including macrophages, in the lamina propria and submucosa of the small intestine. The lamina propria just below the epithelium of the villi was intensely stained red, suggesting that acid-fast bacilli were compacted in macrophages within this area (Fig. 2C). In the adjacent tissues of the acid-fast stained section, galectin-3 was strongly expressed in the round cells (typical of macrophages) in the lamina propria and submucosa of the intestines (Fig. 2B) and moderately expressed in the intestinal epithelium. The intense staining patterns of galectin-3 in the villous lamina propria (Fig. 2D) largely overlapped with the acid-fast staining (Fig. 2C).

Bottom Line: The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease.Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum.These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.

Show MeSH
Related in: MedlinePlus