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Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand.

Pohuang T, Chansiripornchai N, Tawatsin A, Sasipreeyajan J - J. Vet. Sci. (2009)

Bottom Line: This group likely represents the strains indigenous to Thailand.The isolates in group II showed a close relationship with Chinese IBVs.They had nucleotide sequence identities of 97-98% and amino acid sequence identities 96-98% in common with Chinese IBVs (strain A2, SH and QXIBV).

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and amino acid sequence identities less than 84% in common with IBVs published in the GenBank database. This group likely represents the strains indigenous to Thailand. The isolates in group II showed a close relationship with Chinese IBVs. They had nucleotide sequence identities of 97-98% and amino acid sequence identities 96-98% in common with Chinese IBVs (strain A2, SH and QXIBV). This finding indicated that the recent Thai IBVs evolved separately and at least two groups of viruses are circulating in Thailand.

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Related in: MedlinePlus

Phylogenetic tree based on the nucleotide sequence between aligned S1 sequences from Thai infectious bronchitis virus isolates and published sequences.
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Figure 1: Phylogenetic tree based on the nucleotide sequence between aligned S1 sequences from Thai infectious bronchitis virus isolates and published sequences.

Mentions: To assess the genetic relationship among the IBV isolates, a phylogenetic tree was constructed from the nucleotide sequences of S1 genes. The results are shown in Fig.1. The thirteen IBV isolates were separated into two distinct groups. Group I consisted of five isolates including THA20151, THA40151, THA50151, THA60151, and THA90151. The isolates in group I showed evolutionary distances from each other. Group II consisted of eight isolates including THA30151, THA 70151, THA 80151, THA100151, THA110351, THA120351, THA130551, and THA140551, which had a close relationship with Chinese IBV isolates (strain A2, SH and QXIBV).


Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand.

Pohuang T, Chansiripornchai N, Tawatsin A, Sasipreeyajan J - J. Vet. Sci. (2009)

Phylogenetic tree based on the nucleotide sequence between aligned S1 sequences from Thai infectious bronchitis virus isolates and published sequences.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801127&req=5

Figure 1: Phylogenetic tree based on the nucleotide sequence between aligned S1 sequences from Thai infectious bronchitis virus isolates and published sequences.
Mentions: To assess the genetic relationship among the IBV isolates, a phylogenetic tree was constructed from the nucleotide sequences of S1 genes. The results are shown in Fig.1. The thirteen IBV isolates were separated into two distinct groups. Group I consisted of five isolates including THA20151, THA40151, THA50151, THA60151, and THA90151. The isolates in group I showed evolutionary distances from each other. Group II consisted of eight isolates including THA30151, THA 70151, THA 80151, THA100151, THA110351, THA120351, THA130551, and THA140551, which had a close relationship with Chinese IBV isolates (strain A2, SH and QXIBV).

Bottom Line: This group likely represents the strains indigenous to Thailand.The isolates in group II showed a close relationship with Chinese IBVs.They had nucleotide sequence identities of 97-98% and amino acid sequence identities 96-98% in common with Chinese IBVs (strain A2, SH and QXIBV).

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and amino acid sequence identities less than 84% in common with IBVs published in the GenBank database. This group likely represents the strains indigenous to Thailand. The isolates in group II showed a close relationship with Chinese IBVs. They had nucleotide sequence identities of 97-98% and amino acid sequence identities 96-98% in common with Chinese IBVs (strain A2, SH and QXIBV). This finding indicated that the recent Thai IBVs evolved separately and at least two groups of viruses are circulating in Thailand.

Show MeSH
Related in: MedlinePlus