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Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).

Gao FS, Hu GX, Xia XZ, Gao YW, Bai YD, Zou XH - J. Vet. Sci. (2009)

Bottom Line: Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo.It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV.The results suggest that the affected pandas had been infected with CCV.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China.

ABSTRACT
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

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Analysis of GPV based on gene sequencing. (A) Comparison of amino acid sequences of S gene product from GPV by nested PCR assay with that of the S gene of canine coronavirus (CCV) K378 (X77047). The asterisk indicates conserved amino acids between GPV and CCV K378; the dot indicates synonymous mutations of amino acids between GPV and CCV K378; the blanks indicate mutant amino acids between GPV to CCV K378; the number after the virus' name corresponds to the amino acid position in the S gene. (B) Percentage of amino acid identity between the partial S gene of GPV and CCV.
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Figure 3: Analysis of GPV based on gene sequencing. (A) Comparison of amino acid sequences of S gene product from GPV by nested PCR assay with that of the S gene of canine coronavirus (CCV) K378 (X77047). The asterisk indicates conserved amino acids between GPV and CCV K378; the dot indicates synonymous mutations of amino acids between GPV and CCV K378; the blanks indicate mutant amino acids between GPV to CCV K378; the number after the virus' name corresponds to the amino acid position in the S gene. (B) Percentage of amino acid identity between the partial S gene of GPV and CCV.

Mentions: The virus was further analyzed at the molecular level using RT-PCR. After RT-PCR a 514 bp fragment was amplified from tissues and infected FCWF cells, while PCR for non-infected FCWF cells yielded no results. By sequencing and analysis, the sequences from tissues and infected FCWF cells showed 100% nucleotide identity. As shown in Fig. 3A, the amino acid sequence of the amplified GPV S gene was 98.7% identical to the S protein of CCV K378. In addition, the sequence of the GPV S gene was also 84.3% to 100% identical to the other strains of CCV, including CCV1-71 (AF116246), CCV6 (A22882), CCV C54 (A22886), CCV INSAVC (D13096), UWSMN-1 (AF327928), CCV TN449 (AF116245), and CCV5821 (AB017789) (Fig. 3B).


Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).

Gao FS, Hu GX, Xia XZ, Gao YW, Bai YD, Zou XH - J. Vet. Sci. (2009)

Analysis of GPV based on gene sequencing. (A) Comparison of amino acid sequences of S gene product from GPV by nested PCR assay with that of the S gene of canine coronavirus (CCV) K378 (X77047). The asterisk indicates conserved amino acids between GPV and CCV K378; the dot indicates synonymous mutations of amino acids between GPV and CCV K378; the blanks indicate mutant amino acids between GPV to CCV K378; the number after the virus' name corresponds to the amino acid position in the S gene. (B) Percentage of amino acid identity between the partial S gene of GPV and CCV.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801125&req=5

Figure 3: Analysis of GPV based on gene sequencing. (A) Comparison of amino acid sequences of S gene product from GPV by nested PCR assay with that of the S gene of canine coronavirus (CCV) K378 (X77047). The asterisk indicates conserved amino acids between GPV and CCV K378; the dot indicates synonymous mutations of amino acids between GPV and CCV K378; the blanks indicate mutant amino acids between GPV to CCV K378; the number after the virus' name corresponds to the amino acid position in the S gene. (B) Percentage of amino acid identity between the partial S gene of GPV and CCV.
Mentions: The virus was further analyzed at the molecular level using RT-PCR. After RT-PCR a 514 bp fragment was amplified from tissues and infected FCWF cells, while PCR for non-infected FCWF cells yielded no results. By sequencing and analysis, the sequences from tissues and infected FCWF cells showed 100% nucleotide identity. As shown in Fig. 3A, the amino acid sequence of the amplified GPV S gene was 98.7% identical to the S protein of CCV K378. In addition, the sequence of the GPV S gene was also 84.3% to 100% identical to the other strains of CCV, including CCV1-71 (AF116246), CCV6 (A22882), CCV C54 (A22886), CCV INSAVC (D13096), UWSMN-1 (AF327928), CCV TN449 (AF116245), and CCV5821 (AB017789) (Fig. 3B).

Bottom Line: Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo.It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV.The results suggest that the affected pandas had been infected with CCV.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China.

ABSTRACT
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

Show MeSH
Related in: MedlinePlus