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Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).

Gao FS, Hu GX, Xia XZ, Gao YW, Bai YD, Zou XH - J. Vet. Sci. (2009)

Bottom Line: Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo.It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV.The results suggest that the affected pandas had been infected with CCV.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China.

ABSTRACT
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

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Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) cell line. (A) Uninfected FCWF cells; (B) FCWF cells inoculated with 10,000 TCID50 GPV. The arrows indicate cells with cytopathic effect, which became round and detatch from the bottom of the culture flask. ×400.
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Figure 1: Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) cell line. (A) Uninfected FCWF cells; (B) FCWF cells inoculated with 10,000 TCID50 GPV. The arrows indicate cells with cytopathic effect, which became round and detatch from the bottom of the culture flask. ×400.

Mentions: Cell cultures displayed CPE after 10 passages. Cell fusions could also be observed when comparing infected cultures with negative controls (Figs. 1A and B). The isolated virus was named as giant panda virus (GPV).


Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).

Gao FS, Hu GX, Xia XZ, Gao YW, Bai YD, Zou XH - J. Vet. Sci. (2009)

Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) cell line. (A) Uninfected FCWF cells; (B) FCWF cells inoculated with 10,000 TCID50 GPV. The arrows indicate cells with cytopathic effect, which became round and detatch from the bottom of the culture flask. ×400.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801125&req=5

Figure 1: Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) cell line. (A) Uninfected FCWF cells; (B) FCWF cells inoculated with 10,000 TCID50 GPV. The arrows indicate cells with cytopathic effect, which became round and detatch from the bottom of the culture flask. ×400.
Mentions: Cell cultures displayed CPE after 10 passages. Cell fusions could also be observed when comparing infected cultures with negative controls (Figs. 1A and B). The isolated virus was named as giant panda virus (GPV).

Bottom Line: Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo.It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV.The results suggest that the affected pandas had been infected with CCV.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China.

ABSTRACT
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

Show MeSH
Related in: MedlinePlus