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Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice.

Hue JJ, Lee KN, Jeong JH, Lee SH, Lee YH, Jeong SW, Nam SY, Yun YW, Lee BJ - J. Vet. Sci. (2009)

Bottom Line: The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05).CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05).DG and DGCLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DGCLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism.

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Microphotographs of the liver of C57BL/6J ob/ob mice. Many fat droplets were diffusely present in the liver of the obese mice in control and treated with test solutions. (A) Control, (B) Diglyceride, (C) Conjugated linoleic acid, (D) Diglyceride containing 22% conjugated linoleic acid. H&E stain, ×200.
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Figure 3: Microphotographs of the liver of C57BL/6J ob/ob mice. Many fat droplets were diffusely present in the liver of the obese mice in control and treated with test solutions. (A) Control, (B) Diglyceride, (C) Conjugated linoleic acid, (D) Diglyceride containing 22% conjugated linoleic acid. H&E stain, ×200.

Mentions: Livers of the control mice (C57BL/6J ob/ob) had numerous lipid droplets compared with those from lean normal mice (C57BL/6J) (data not shown). Large fat droplets were present diffusely in the liver of the control obese mice (Fig. 3). The treatment CLA and DG-CLA showed a reasonable decrease in the number of lipid droplets compared to the control (Fig. 3). In the liver stained with oil red O, a remarkable decrease in lipid droplets by CLA and DGCLA treatments were clearly shown, compared with the control (Fig. 4). Treatment of DG also weakly ameliorated the lipid accumulation in the liver cells compared with the control (Fig. 4).


Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice.

Hue JJ, Lee KN, Jeong JH, Lee SH, Lee YH, Jeong SW, Nam SY, Yun YW, Lee BJ - J. Vet. Sci. (2009)

Microphotographs of the liver of C57BL/6J ob/ob mice. Many fat droplets were diffusely present in the liver of the obese mice in control and treated with test solutions. (A) Control, (B) Diglyceride, (C) Conjugated linoleic acid, (D) Diglyceride containing 22% conjugated linoleic acid. H&E stain, ×200.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801123&req=5

Figure 3: Microphotographs of the liver of C57BL/6J ob/ob mice. Many fat droplets were diffusely present in the liver of the obese mice in control and treated with test solutions. (A) Control, (B) Diglyceride, (C) Conjugated linoleic acid, (D) Diglyceride containing 22% conjugated linoleic acid. H&E stain, ×200.
Mentions: Livers of the control mice (C57BL/6J ob/ob) had numerous lipid droplets compared with those from lean normal mice (C57BL/6J) (data not shown). Large fat droplets were present diffusely in the liver of the control obese mice (Fig. 3). The treatment CLA and DG-CLA showed a reasonable decrease in the number of lipid droplets compared to the control (Fig. 3). In the liver stained with oil red O, a remarkable decrease in lipid droplets by CLA and DGCLA treatments were clearly shown, compared with the control (Fig. 4). Treatment of DG also weakly ameliorated the lipid accumulation in the liver cells compared with the control (Fig. 4).

Bottom Line: The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05).CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05).DG and DGCLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DGCLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism.

Show MeSH
Related in: MedlinePlus