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Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells.

Soliman MM, El-Senosi YA, Salem MM, Abdel Hamid OM, Kazuhiro K - J. Vet. Sci. (2009)

Bottom Line: Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation.Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation.The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 020-013, Egypt. mohamedsoliman8896@yahoo.com

ABSTRACT
Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microg/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.

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Effect of PI on ASP stimulated lipids accumulation in 3T3-L1 cells. Mature cells were incubated for 4 days as described in Fig. 4. Lipid accumulation was measured spectrophotmetrically at OD 540 nm. The lipids were removed from cells after staining by oil red O and removal by isopropanol. Values are means ± SE obtained from 3 experiments. *p < 0.05 compared to control, †p < 0.05 compared to insulin, ‡p < 0.05 compared to insulin plus PI and, §p < 0.05 compared to insulin and insulin plus ASP.
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Figure 5: Effect of PI on ASP stimulated lipids accumulation in 3T3-L1 cells. Mature cells were incubated for 4 days as described in Fig. 4. Lipid accumulation was measured spectrophotmetrically at OD 540 nm. The lipids were removed from cells after staining by oil red O and removal by isopropanol. Values are means ± SE obtained from 3 experiments. *p < 0.05 compared to control, †p < 0.05 compared to insulin, ‡p < 0.05 compared to insulin plus PI and, §p < 0.05 compared to insulin and insulin plus ASP.

Mentions: To test that effect, cells were incubated with insulin and as seen in Fig. 4, the level of adipocytes differentiation and lipids accumulation was increased. Moreover, when it incubated with ASP in high dose (450 ng/mL) together with insulin, there was an additive increase in lipids accumulation was seen (Fig. 4). When the cells were incubated in presence of insulin and PI, TG accumulation was decreased. When ASP in low, medium and high doses of ASP was added together with insulin and PI, TG accumulation was partially reversed when compared with that of insulin and PI together (Figs. 4 and 5).


Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells.

Soliman MM, El-Senosi YA, Salem MM, Abdel Hamid OM, Kazuhiro K - J. Vet. Sci. (2009)

Effect of PI on ASP stimulated lipids accumulation in 3T3-L1 cells. Mature cells were incubated for 4 days as described in Fig. 4. Lipid accumulation was measured spectrophotmetrically at OD 540 nm. The lipids were removed from cells after staining by oil red O and removal by isopropanol. Values are means ± SE obtained from 3 experiments. *p < 0.05 compared to control, †p < 0.05 compared to insulin, ‡p < 0.05 compared to insulin plus PI and, §p < 0.05 compared to insulin and insulin plus ASP.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801122&req=5

Figure 5: Effect of PI on ASP stimulated lipids accumulation in 3T3-L1 cells. Mature cells were incubated for 4 days as described in Fig. 4. Lipid accumulation was measured spectrophotmetrically at OD 540 nm. The lipids were removed from cells after staining by oil red O and removal by isopropanol. Values are means ± SE obtained from 3 experiments. *p < 0.05 compared to control, †p < 0.05 compared to insulin, ‡p < 0.05 compared to insulin plus PI and, §p < 0.05 compared to insulin and insulin plus ASP.
Mentions: To test that effect, cells were incubated with insulin and as seen in Fig. 4, the level of adipocytes differentiation and lipids accumulation was increased. Moreover, when it incubated with ASP in high dose (450 ng/mL) together with insulin, there was an additive increase in lipids accumulation was seen (Fig. 4). When the cells were incubated in presence of insulin and PI, TG accumulation was decreased. When ASP in low, medium and high doses of ASP was added together with insulin and PI, TG accumulation was partially reversed when compared with that of insulin and PI together (Figs. 4 and 5).

Bottom Line: Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation.Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation.The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 020-013, Egypt. mohamedsoliman8896@yahoo.com

ABSTRACT
Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microg/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.

Show MeSH
Related in: MedlinePlus