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Low dietary inorganic phosphate affects the lung growth of developing mice.

Xu CX, Jin H, Chung YS, Shin JY, Hwang SK, Kwon JT, Park SJ, Lee ES, Minai-Tehrani A, Chang SH, Woo MA, Noh MS, An GH, Lee KH, Cho MH - J. Vet. Sci. (2009)

Bottom Line: Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene.Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2.These results suggest that optimally regulating Pi consumption may be important to maintain health.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.

ABSTRACT
Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.

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Analysis of fibroblast growth factor 2 (FGF-2) protein in the lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expression of FGF-2 protein in the lung. (B) The bands-of-interests were further analyzed by using a densitometer. (C) Immunohistochemical measurement of FGF-2 in the lung of transgenic mice. The dark brown color indicates the expression of FGF-2 (scale bar = 100 µm). (D) Comparison of the FGF-2 labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).
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Figure 5: Analysis of fibroblast growth factor 2 (FGF-2) protein in the lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expression of FGF-2 protein in the lung. (B) The bands-of-interests were further analyzed by using a densitometer. (C) Immunohistochemical measurement of FGF-2 in the lung of transgenic mice. The dark brown color indicates the expression of FGF-2 (scale bar = 100 µm). (D) Comparison of the FGF-2 labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).

Mentions: Low dietary Pi significantly increased the FGF-2 protein expression as shown on Western blotting and densitometric analysis (Figs. 5A and B). Such an overexpression of FGF-2 was clearly demonstrated by the IHC study. As shown Figs. 5C and D, FGF-2 expression was increased about 7.5 fold in the low Pi diet group than control group.


Low dietary inorganic phosphate affects the lung growth of developing mice.

Xu CX, Jin H, Chung YS, Shin JY, Hwang SK, Kwon JT, Park SJ, Lee ES, Minai-Tehrani A, Chang SH, Woo MA, Noh MS, An GH, Lee KH, Cho MH - J. Vet. Sci. (2009)

Analysis of fibroblast growth factor 2 (FGF-2) protein in the lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expression of FGF-2 protein in the lung. (B) The bands-of-interests were further analyzed by using a densitometer. (C) Immunohistochemical measurement of FGF-2 in the lung of transgenic mice. The dark brown color indicates the expression of FGF-2 (scale bar = 100 µm). (D) Comparison of the FGF-2 labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801121&req=5

Figure 5: Analysis of fibroblast growth factor 2 (FGF-2) protein in the lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expression of FGF-2 protein in the lung. (B) The bands-of-interests were further analyzed by using a densitometer. (C) Immunohistochemical measurement of FGF-2 in the lung of transgenic mice. The dark brown color indicates the expression of FGF-2 (scale bar = 100 µm). (D) Comparison of the FGF-2 labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).
Mentions: Low dietary Pi significantly increased the FGF-2 protein expression as shown on Western blotting and densitometric analysis (Figs. 5A and B). Such an overexpression of FGF-2 was clearly demonstrated by the IHC study. As shown Figs. 5C and D, FGF-2 expression was increased about 7.5 fold in the low Pi diet group than control group.

Bottom Line: Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene.Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2.These results suggest that optimally regulating Pi consumption may be important to maintain health.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.

ABSTRACT
Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.

Show MeSH