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Low dietary inorganic phosphate affects the lung growth of developing mice.

Xu CX, Jin H, Chung YS, Shin JY, Hwang SK, Kwon JT, Park SJ, Lee ES, Minai-Tehrani A, Chang SH, Woo MA, Noh MS, An GH, Lee KH, Cho MH - J. Vet. Sci. (2009)

Bottom Line: Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene.Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2.These results suggest that optimally regulating Pi consumption may be important to maintain health.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.

ABSTRACT
Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.

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Western blot analysis of the cell cycle signaling proteins. The lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expressions of p53, p21 and p27 protein in lung. (B) The expressions of cyclin D3, cyclin-dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA) protein in lung. (C, D) The bands-of-interests were further analyzed by using a densitometer. (E) Immunohistochemical measurement of PCNA in the lung. The dark brown color indicates the PCNA expression (scale bar = 100 µm). (F) Comparison of the PCNA labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).
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Figure 4: Western blot analysis of the cell cycle signaling proteins. The lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expressions of p53, p21 and p27 protein in lung. (B) The expressions of cyclin D3, cyclin-dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA) protein in lung. (C, D) The bands-of-interests were further analyzed by using a densitometer. (E) Immunohistochemical measurement of PCNA in the lung. The dark brown color indicates the PCNA expression (scale bar = 100 µm). (F) Comparison of the PCNA labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).

Mentions: Low Pi decreased the protein expressions of p53, p21 and p27 (Figs. 4A and C). In contrast, low Pi significantly increased the protein expressions of cyclin D3, CDK4 and PCNA (Figs. 4B and D). IHC analysis of PCNA clearly showed that low dietary Pi stimulated lung cell proliferation in the lungs of the dual luciferase reporter mice (Figs. 4E and F).


Low dietary inorganic phosphate affects the lung growth of developing mice.

Xu CX, Jin H, Chung YS, Shin JY, Hwang SK, Kwon JT, Park SJ, Lee ES, Minai-Tehrani A, Chang SH, Woo MA, Noh MS, An GH, Lee KH, Cho MH - J. Vet. Sci. (2009)

Western blot analysis of the cell cycle signaling proteins. The lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expressions of p53, p21 and p27 protein in lung. (B) The expressions of cyclin D3, cyclin-dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA) protein in lung. (C, D) The bands-of-interests were further analyzed by using a densitometer. (E) Immunohistochemical measurement of PCNA in the lung. The dark brown color indicates the PCNA expression (scale bar = 100 µm). (F) Comparison of the PCNA labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801121&req=5

Figure 4: Western blot analysis of the cell cycle signaling proteins. The lungs of mice fed a low Pi diet (0.1% Pi) or a normal (0.5% Pi) diet for 4 weeks. (A) The expressions of p53, p21 and p27 protein in lung. (B) The expressions of cyclin D3, cyclin-dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA) protein in lung. (C, D) The bands-of-interests were further analyzed by using a densitometer. (E) Immunohistochemical measurement of PCNA in the lung. The dark brown color indicates the PCNA expression (scale bar = 100 µm). (F) Comparison of the PCNA labeling index in the lungs. p values (*p < 0.05, **p < 0.01) indicate a significant difference compared with normal (mean ± SE, n = 4).
Mentions: Low Pi decreased the protein expressions of p53, p21 and p27 (Figs. 4A and C). In contrast, low Pi significantly increased the protein expressions of cyclin D3, CDK4 and PCNA (Figs. 4B and D). IHC analysis of PCNA clearly showed that low dietary Pi stimulated lung cell proliferation in the lungs of the dual luciferase reporter mice (Figs. 4E and F).

Bottom Line: Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene.Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2.These results suggest that optimally regulating Pi consumption may be important to maintain health.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.

ABSTRACT
Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.

Show MeSH