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Stereolithographic biomodeling of equine ovary based on 3D serial digitizing device.

Kimura J, Kakusho N, Yamazawa K, Hirano Y, Nambo Y, Yokota H, Himeno R - J. Vet. Sci. (2009)

Bottom Line: Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary.The understanding of the spatial relationship between the ovulation fossa and follicles and/or corpora lutea in the equine ovary was a great benefit.The peculiar structure of the equine ovary could be thoroughly observed and understood through this model.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Seoul National University, Seoul, Korea. kimura@snu.ac.kr

ABSTRACT
The 3D internal structure microscopy (3D-ISM) was applied to the equine ovary, which possesses peculiar structural characteristics. Stereolithography was applied to make a life-sized model by means of data obtained from 3D-ISM. Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary. Photopolymerized resin models of equine ovaries produced by stereolithography can clearly show the internal structure and spatial localizations in the ovary. The understanding of the spatial relationship between the ovulation fossa and follicles and/or corpora lutea in the equine ovary was a great benefit. The peculiar structure of the equine ovary could be thoroughly observed and understood through this model.

Show MeSH
The computer simulation of the spatial arrangement of follicles in the equine ovary. These images were created by 3-dimensional reconstruction from serially sliced images. Assuming that the ovary is in the hexahedron box, six views are illustrated from the bottom surface (A), the top surface (B), the front surface (C), the back surface (D), the left side surface (E) and the right side surface (F). Follicles whose size is bigger than 10,000 mm3 marked in red, 3,000~9,999 mm3 marked in green, 1,000~2,999 mm3 marked in blue and 100~999 mm3 marked in white.
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Figure 1: The computer simulation of the spatial arrangement of follicles in the equine ovary. These images were created by 3-dimensional reconstruction from serially sliced images. Assuming that the ovary is in the hexahedron box, six views are illustrated from the bottom surface (A), the top surface (B), the front surface (C), the back surface (D), the left side surface (E) and the right side surface (F). Follicles whose size is bigger than 10,000 mm3 marked in red, 3,000~9,999 mm3 marked in green, 1,000~2,999 mm3 marked in blue and 100~999 mm3 marked in white.

Mentions: Two equine ovaries, one with follicles in varying sizes and the other with a large corpora luteum, were processed to make serially sliced images by three dimensional internal microscopy, and more than a thousand sliced images were obtained and stored as digital data. The size and the spatial localization of the follicle and corpus luteum were expressed by computational simulation with the aid of an RV editor (Riken, Japan) after analysis by 3D internal microscopy. Fig. 1 shows the location and size of the follicles in variable sizes in the ovary in the follicular phase. Six surfaces can be seen from different angles. Colors indicate the size of the follicles as mentioned previously in the paper. Based on the digital data obtained by image processing, manufacture of the stereolithographs of equine ovaries with the aid of the laser stereolithography device was successful in these two examples (Fig. 2). The model is to a 1 : 1 scale and the space for the follicles or corpus luteum was created. The injection of dye-silicone mixtures into these spaces was successful through the narrow passages specifically made for this purpose. The injected dyes into the empty spaces were very effective for visualization of the follicles (Fig. 2B) and corpus luteum (Fig. 2C).


Stereolithographic biomodeling of equine ovary based on 3D serial digitizing device.

Kimura J, Kakusho N, Yamazawa K, Hirano Y, Nambo Y, Yokota H, Himeno R - J. Vet. Sci. (2009)

The computer simulation of the spatial arrangement of follicles in the equine ovary. These images were created by 3-dimensional reconstruction from serially sliced images. Assuming that the ovary is in the hexahedron box, six views are illustrated from the bottom surface (A), the top surface (B), the front surface (C), the back surface (D), the left side surface (E) and the right side surface (F). Follicles whose size is bigger than 10,000 mm3 marked in red, 3,000~9,999 mm3 marked in green, 1,000~2,999 mm3 marked in blue and 100~999 mm3 marked in white.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801120&req=5

Figure 1: The computer simulation of the spatial arrangement of follicles in the equine ovary. These images were created by 3-dimensional reconstruction from serially sliced images. Assuming that the ovary is in the hexahedron box, six views are illustrated from the bottom surface (A), the top surface (B), the front surface (C), the back surface (D), the left side surface (E) and the right side surface (F). Follicles whose size is bigger than 10,000 mm3 marked in red, 3,000~9,999 mm3 marked in green, 1,000~2,999 mm3 marked in blue and 100~999 mm3 marked in white.
Mentions: Two equine ovaries, one with follicles in varying sizes and the other with a large corpora luteum, were processed to make serially sliced images by three dimensional internal microscopy, and more than a thousand sliced images were obtained and stored as digital data. The size and the spatial localization of the follicle and corpus luteum were expressed by computational simulation with the aid of an RV editor (Riken, Japan) after analysis by 3D internal microscopy. Fig. 1 shows the location and size of the follicles in variable sizes in the ovary in the follicular phase. Six surfaces can be seen from different angles. Colors indicate the size of the follicles as mentioned previously in the paper. Based on the digital data obtained by image processing, manufacture of the stereolithographs of equine ovaries with the aid of the laser stereolithography device was successful in these two examples (Fig. 2). The model is to a 1 : 1 scale and the space for the follicles or corpus luteum was created. The injection of dye-silicone mixtures into these spaces was successful through the narrow passages specifically made for this purpose. The injected dyes into the empty spaces were very effective for visualization of the follicles (Fig. 2B) and corpus luteum (Fig. 2C).

Bottom Line: Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary.The understanding of the spatial relationship between the ovulation fossa and follicles and/or corpora lutea in the equine ovary was a great benefit.The peculiar structure of the equine ovary could be thoroughly observed and understood through this model.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Seoul National University, Seoul, Korea. kimura@snu.ac.kr

ABSTRACT
The 3D internal structure microscopy (3D-ISM) was applied to the equine ovary, which possesses peculiar structural characteristics. Stereolithography was applied to make a life-sized model by means of data obtained from 3D-ISM. Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary. Photopolymerized resin models of equine ovaries produced by stereolithography can clearly show the internal structure and spatial localizations in the ovary. The understanding of the spatial relationship between the ovulation fossa and follicles and/or corpora lutea in the equine ovary was a great benefit. The peculiar structure of the equine ovary could be thoroughly observed and understood through this model.

Show MeSH