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Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms.

Kim HK, Yang JS, Moon HJ, Park SJ, Luo Y, Lee CS, Song DS, Kang BK, Ann SK, Jun CH, Park BK - J. Vet. Sci. (2009)

Bottom Line: The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4.Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%).The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea.

ABSTRACT
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2 approximately 88.9% similarity with the Lelystad virus, showing a close relationship with the 27 approximately 2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.

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Amino acid sequences of two type II PRRSV isolates and 23 type II PRRSV ORF5 in the viremic sera from 2007 to 2008. The amino acid sequences were compared with the sequence of MLV. The known B-cell epitopes, epitope A (decoying epitope, 27~30), epitope B (neutralizing epitope, 37~45) and recently identified region (187~200) were indicated by small, medium and large boxes, respectively. Furthermore, the two T-cell epitope regions of GP5 were also indicated by grey backgrounds.
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Figure 3: Amino acid sequences of two type II PRRSV isolates and 23 type II PRRSV ORF5 in the viremic sera from 2007 to 2008. The amino acid sequences were compared with the sequence of MLV. The known B-cell epitopes, epitope A (decoying epitope, 27~30), epitope B (neutralizing epitope, 37~45) and recently identified region (187~200) were indicated by small, medium and large boxes, respectively. Furthermore, the two T-cell epitope regions of GP5 were also indicated by grey backgrounds.

Mentions: The amino acid sequences of 23 type II PRRSV ORF5 in the viremic sera taken from 2007 to 2008 were aligned to compare several epitope regions (Fig. 3). The Ksg-4-containing subgroup had at least one mutated sequences at H38, L39, L41 and N44 (K/Q38, F/I39, S41 and K44). In the case of the 3rd B-cell epitope (182~200), I189, R191 and Q196 were frequently replaced by V189, K191 and R196 in those groups. The MN184-like subgroup showed the replacement of V185 and R191 to A185 and K191. The first T-cell epitope (117~131) was also a variable region among Korean type II PRRSVs obtained from viremic sera. Most common was V124 and A128 being dominantly replaced by T/I124 and V/T128 in the Ksg-4-containing subgroup. Notably, the ORF5s in MN184-like subgroup had common mutations (FA127-128 → LT127-128) which were also observed in the isolate CP07-626-2 belonging to this subgroup. The second T-cell epitope (149~163) was more conserved than the first T-cell epitope (127~131) in the ORF5 sequences used in this study. The obvious differences of epitope regions were not observed between MLV-vaccinating or non-vaccinating farms, comparing the viruses in same subgroup.


Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms.

Kim HK, Yang JS, Moon HJ, Park SJ, Luo Y, Lee CS, Song DS, Kang BK, Ann SK, Jun CH, Park BK - J. Vet. Sci. (2009)

Amino acid sequences of two type II PRRSV isolates and 23 type II PRRSV ORF5 in the viremic sera from 2007 to 2008. The amino acid sequences were compared with the sequence of MLV. The known B-cell epitopes, epitope A (decoying epitope, 27~30), epitope B (neutralizing epitope, 37~45) and recently identified region (187~200) were indicated by small, medium and large boxes, respectively. Furthermore, the two T-cell epitope regions of GP5 were also indicated by grey backgrounds.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801115&req=5

Figure 3: Amino acid sequences of two type II PRRSV isolates and 23 type II PRRSV ORF5 in the viremic sera from 2007 to 2008. The amino acid sequences were compared with the sequence of MLV. The known B-cell epitopes, epitope A (decoying epitope, 27~30), epitope B (neutralizing epitope, 37~45) and recently identified region (187~200) were indicated by small, medium and large boxes, respectively. Furthermore, the two T-cell epitope regions of GP5 were also indicated by grey backgrounds.
Mentions: The amino acid sequences of 23 type II PRRSV ORF5 in the viremic sera taken from 2007 to 2008 were aligned to compare several epitope regions (Fig. 3). The Ksg-4-containing subgroup had at least one mutated sequences at H38, L39, L41 and N44 (K/Q38, F/I39, S41 and K44). In the case of the 3rd B-cell epitope (182~200), I189, R191 and Q196 were frequently replaced by V189, K191 and R196 in those groups. The MN184-like subgroup showed the replacement of V185 and R191 to A185 and K191. The first T-cell epitope (117~131) was also a variable region among Korean type II PRRSVs obtained from viremic sera. Most common was V124 and A128 being dominantly replaced by T/I124 and V/T128 in the Ksg-4-containing subgroup. Notably, the ORF5s in MN184-like subgroup had common mutations (FA127-128 → LT127-128) which were also observed in the isolate CP07-626-2 belonging to this subgroup. The second T-cell epitope (149~163) was more conserved than the first T-cell epitope (127~131) in the ORF5 sequences used in this study. The obvious differences of epitope regions were not observed between MLV-vaccinating or non-vaccinating farms, comparing the viruses in same subgroup.

Bottom Line: The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4.Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%).The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea.

ABSTRACT
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2 approximately 88.9% similarity with the Lelystad virus, showing a close relationship with the 27 approximately 2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.

Show MeSH
Related in: MedlinePlus