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Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms.

Kim HK, Yang JS, Moon HJ, Park SJ, Luo Y, Lee CS, Song DS, Kang BK, Ann SK, Jun CH, Park BK - J. Vet. Sci. (2009)

Bottom Line: The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4.Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%).The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea.

ABSTRACT
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2 approximately 88.9% similarity with the Lelystad virus, showing a close relationship with the 27 approximately 2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.

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Maximum parsimony tree of type II Korean PRRSV ORF5 from 1997 to 2008. The Lelystad virus, which was a prototype of type I PRRSV, was used as an outgroup. In the maximum parsimony tree, type II Korean PRRSV were mainly divided into four subgroups, subgroup 1, 2, 3 and 4. Ksg-1 and 2 were in the same group, subgroup 1, in this study. Subgroup 2 only contained samples from 2000 to 2003. The MN184-like subgroup was shown to belong to the subgroup 3 which had not been classified before in Korea.
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Figure 2: Maximum parsimony tree of type II Korean PRRSV ORF5 from 1997 to 2008. The Lelystad virus, which was a prototype of type I PRRSV, was used as an outgroup. In the maximum parsimony tree, type II Korean PRRSV were mainly divided into four subgroups, subgroup 1, 2, 3 and 4. Ksg-1 and 2 were in the same group, subgroup 1, in this study. Subgroup 2 only contained samples from 2000 to 2003. The MN184-like subgroup was shown to belong to the subgroup 3 which had not been classified before in Korea.

Mentions: To observe the evolutionary pattern of type II PRRSV from 1997 to 2008, a Maximum parsimony tree was drawn (Fig. 2). The type II Korean PRRSVs were shown to have been evolved into four subgroups, subgroups 1, 2, 3 and 4. Subgroup 1 included a PL97-1, a prototype of type II Korean PRRSV. The previously reported Ksg-1 and 2 subgroups were shown to belong to subgroup 1 and 24% (6/25) of recent type II Korean PRRSVs from 2007 to 2008, were also included in this group. Subgroup 2 containing Ksg-3 had only PRRSVs from samples taken as of 2000 to 2003. The subgroup 3 formed a novel cluster which was not reported before in Korea and had a MN184-like cluster. 28% (7/25) of recent PRRSVs belonged to this subgroup. Subgroup 4 consisted of 48% (12/25) of recent PRRSVs and was consistent with previously reports of the Ksg-4 subgroup.


Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms.

Kim HK, Yang JS, Moon HJ, Park SJ, Luo Y, Lee CS, Song DS, Kang BK, Ann SK, Jun CH, Park BK - J. Vet. Sci. (2009)

Maximum parsimony tree of type II Korean PRRSV ORF5 from 1997 to 2008. The Lelystad virus, which was a prototype of type I PRRSV, was used as an outgroup. In the maximum parsimony tree, type II Korean PRRSV were mainly divided into four subgroups, subgroup 1, 2, 3 and 4. Ksg-1 and 2 were in the same group, subgroup 1, in this study. Subgroup 2 only contained samples from 2000 to 2003. The MN184-like subgroup was shown to belong to the subgroup 3 which had not been classified before in Korea.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801115&req=5

Figure 2: Maximum parsimony tree of type II Korean PRRSV ORF5 from 1997 to 2008. The Lelystad virus, which was a prototype of type I PRRSV, was used as an outgroup. In the maximum parsimony tree, type II Korean PRRSV were mainly divided into four subgroups, subgroup 1, 2, 3 and 4. Ksg-1 and 2 were in the same group, subgroup 1, in this study. Subgroup 2 only contained samples from 2000 to 2003. The MN184-like subgroup was shown to belong to the subgroup 3 which had not been classified before in Korea.
Mentions: To observe the evolutionary pattern of type II PRRSV from 1997 to 2008, a Maximum parsimony tree was drawn (Fig. 2). The type II Korean PRRSVs were shown to have been evolved into four subgroups, subgroups 1, 2, 3 and 4. Subgroup 1 included a PL97-1, a prototype of type II Korean PRRSV. The previously reported Ksg-1 and 2 subgroups were shown to belong to subgroup 1 and 24% (6/25) of recent type II Korean PRRSVs from 2007 to 2008, were also included in this group. Subgroup 2 containing Ksg-3 had only PRRSVs from samples taken as of 2000 to 2003. The subgroup 3 formed a novel cluster which was not reported before in Korea and had a MN184-like cluster. 28% (7/25) of recent PRRSVs belonged to this subgroup. Subgroup 4 consisted of 48% (12/25) of recent PRRSVs and was consistent with previously reports of the Ksg-4 subgroup.

Bottom Line: The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4.Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%).The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea.

ABSTRACT
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2 approximately 88.9% similarity with the Lelystad virus, showing a close relationship with the 27 approximately 2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.

Show MeSH
Related in: MedlinePlus