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Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms.

Kim HK, Yang JS, Moon HJ, Park SJ, Luo Y, Lee CS, Song DS, Kang BK, Ann SK, Jun CH, Park BK - J. Vet. Sci. (2009)

Bottom Line: The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4.Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%).The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea.

ABSTRACT
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2 approximately 88.9% similarity with the Lelystad virus, showing a close relationship with the 27 approximately 2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.

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The neighbor-joining (N-J) tree of open reading frame (ORF) 5 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) in viremic pigs from 2007 to 2008. The name of viremic sera sequences were presented as 'I.D-V (or not)-Year', where the 'V' means the PRRSV ORF5 sequences were from a vaccinating farm. Korean specific subgroup (Ksg)-1, 2, 3 and 4 indicates the phylogenetic groups which were grouped as Korean PRRSV-specific subgroup when compared with Asian isolates in the N-J method of a previous paper [7].
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Figure 1: The neighbor-joining (N-J) tree of open reading frame (ORF) 5 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) in viremic pigs from 2007 to 2008. The name of viremic sera sequences were presented as 'I.D-V (or not)-Year', where the 'V' means the PRRSV ORF5 sequences were from a vaccinating farm. Korean specific subgroup (Ksg)-1, 2, 3 and 4 indicates the phylogenetic groups which were grouped as Korean PRRSV-specific subgroup when compared with Asian isolates in the N-J method of a previous paper [7].

Mentions: The neighbor-joining tree was presented in Fig. 1. Korean specific subgroups (Ksg)-1, 2 and subgroup 3 were clustered near the VR-2332-like subgroup. The majority of the PRRSV samples collected from 2007 to 2008 were shown to belong to Ksg-4, which was previously reported by Cha et al. [7]. There was no subgroup-specific differences between viruses from MLV-vaccinating or non-vaccinating farms. The viruses of the Ksg-4 subgroup had 87.8-89.0% of nucleotide similarity with VR-2332 in the ORF5 region. The recent Korean PRRSVs from 2007 to 2008 made a novel subgroup which was not reported by Cha et al. [7] and were in the same cluster with MN184 strain from USA. The PRRSVs in this subgroup (MN184-like) had a similarity of 90.3~90.5% compared to MN184 and 84.9~87.2% compared to the MLV on nucleotides of the ORF5 region. The type I Korean PRRSVs were clustered with a 27-2003 strain from Spain with 89.1~91.7% of similarity. The Korean type I PRRSV had 84.9~88.4% of similarity in ORF5 when compared with a European-like PRRSV strain (SD-02-11, Genbank Accession no. AY395078) isolated from USA.


Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms.

Kim HK, Yang JS, Moon HJ, Park SJ, Luo Y, Lee CS, Song DS, Kang BK, Ann SK, Jun CH, Park BK - J. Vet. Sci. (2009)

The neighbor-joining (N-J) tree of open reading frame (ORF) 5 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) in viremic pigs from 2007 to 2008. The name of viremic sera sequences were presented as 'I.D-V (or not)-Year', where the 'V' means the PRRSV ORF5 sequences were from a vaccinating farm. Korean specific subgroup (Ksg)-1, 2, 3 and 4 indicates the phylogenetic groups which were grouped as Korean PRRSV-specific subgroup when compared with Asian isolates in the N-J method of a previous paper [7].
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801115&req=5

Figure 1: The neighbor-joining (N-J) tree of open reading frame (ORF) 5 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) in viremic pigs from 2007 to 2008. The name of viremic sera sequences were presented as 'I.D-V (or not)-Year', where the 'V' means the PRRSV ORF5 sequences were from a vaccinating farm. Korean specific subgroup (Ksg)-1, 2, 3 and 4 indicates the phylogenetic groups which were grouped as Korean PRRSV-specific subgroup when compared with Asian isolates in the N-J method of a previous paper [7].
Mentions: The neighbor-joining tree was presented in Fig. 1. Korean specific subgroups (Ksg)-1, 2 and subgroup 3 were clustered near the VR-2332-like subgroup. The majority of the PRRSV samples collected from 2007 to 2008 were shown to belong to Ksg-4, which was previously reported by Cha et al. [7]. There was no subgroup-specific differences between viruses from MLV-vaccinating or non-vaccinating farms. The viruses of the Ksg-4 subgroup had 87.8-89.0% of nucleotide similarity with VR-2332 in the ORF5 region. The recent Korean PRRSVs from 2007 to 2008 made a novel subgroup which was not reported by Cha et al. [7] and were in the same cluster with MN184 strain from USA. The PRRSVs in this subgroup (MN184-like) had a similarity of 90.3~90.5% compared to MN184 and 84.9~87.2% compared to the MLV on nucleotides of the ORF5 region. The type I Korean PRRSVs were clustered with a 27-2003 strain from Spain with 89.1~91.7% of similarity. The Korean type I PRRSV had 84.9~88.4% of similarity in ORF5 when compared with a European-like PRRSV strain (SD-02-11, Genbank Accession no. AY395078) isolated from USA.

Bottom Line: The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4.Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%).The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea.

ABSTRACT
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2 approximately 88.9% similarity with the Lelystad virus, showing a close relationship with the 27 approximately 2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.

Show MeSH
Related in: MedlinePlus