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A novel beta-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages.

Chang ZQ, Lee JS, Hwang MH, Hong JH, Jung HK, Lee SP, Park SC - J. Vet. Sci. (2009)

Bottom Line: The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner.Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.

ABSTRACT
The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner. Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.

Show MeSH
β-glucan induced mRNA expression of cytokines in RAW264.7 macrophages. RAW264.7 cells were exposed to β-glucan at various concentrations, or LPS. After an 8 h incubation, i-NOS, COX-2, IL-6 and TNF-α mRNA were assessed by semi-quantitative RT-PCR.
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Figure 5: β-glucan induced mRNA expression of cytokines in RAW264.7 macrophages. RAW264.7 cells were exposed to β-glucan at various concentrations, or LPS. After an 8 h incubation, i-NOS, COX-2, IL-6 and TNF-α mRNA were assessed by semi-quantitative RT-PCR.

Mentions: Finally, the mRNA expression of various cytokines was investigated in RAW264.7 macrophages which were exposed to β-glucan or LPS. P. polymyxa JB115 β-glucan induced mRNA expressions of i-NOS in a concentration-dependent manner, which might play a key role in NO production. A similar result was also observed for the mRNA expression of COX-2 and IL-6 (Fig. 5).


A novel beta-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages.

Chang ZQ, Lee JS, Hwang MH, Hong JH, Jung HK, Lee SP, Park SC - J. Vet. Sci. (2009)

β-glucan induced mRNA expression of cytokines in RAW264.7 macrophages. RAW264.7 cells were exposed to β-glucan at various concentrations, or LPS. After an 8 h incubation, i-NOS, COX-2, IL-6 and TNF-α mRNA were assessed by semi-quantitative RT-PCR.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801109&req=5

Figure 5: β-glucan induced mRNA expression of cytokines in RAW264.7 macrophages. RAW264.7 cells were exposed to β-glucan at various concentrations, or LPS. After an 8 h incubation, i-NOS, COX-2, IL-6 and TNF-α mRNA were assessed by semi-quantitative RT-PCR.
Mentions: Finally, the mRNA expression of various cytokines was investigated in RAW264.7 macrophages which were exposed to β-glucan or LPS. P. polymyxa JB115 β-glucan induced mRNA expressions of i-NOS in a concentration-dependent manner, which might play a key role in NO production. A similar result was also observed for the mRNA expression of COX-2 and IL-6 (Fig. 5).

Bottom Line: The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner.Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.

ABSTRACT
The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner. Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.

Show MeSH