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A suspected case of Lyme borreliosis in a hunting dog in Korea.

Choi US, Kim HW, You SE, Youn HJ - J. Vet. Sci. (2009)

Bottom Line: Spleen was enlarged by radiography, and the lymph nodes showed neutrophilic lymphadenitis by cytological examination.A polymerase chain reaction test for babesiosis and commercial ELISA tests for Ehrlichia canis, heartworm, and Lyme disease was negative except for Lyme disease, which was verified by both an IFA-IgG test and a quantitative C(6) assay.Post-treatment C(6) titer decreased to within normal limits.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Korea.

ABSTRACT
A two-year-old male Pointer had been presented with anorexia, cachexia, and weight loss of 10-day duration. Upon physical examination, fever, lethargy, superficial lymph node enlargement, and tick infestation were noted. The only abnormality in CBC and serum chemistry analyses was mild hyperglobulinemia. Spleen was enlarged by radiography, and the lymph nodes showed neutrophilic lymphadenitis by cytological examination. A polymerase chain reaction test for babesiosis and commercial ELISA tests for Ehrlichia canis, heartworm, and Lyme disease was negative except for Lyme disease, which was verified by both an IFA-IgG test and a quantitative C(6) assay. Doxycycline was administered for 2 weeks and the recovery was uneventful. Post-treatment C(6) titer decreased to within normal limits.

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Radiograph of the spleen. The abdomen was decreased by lack of fat. In the lateral view, soft tissue density structure (arrows) was identified in the ventral region of the middle abdomen, and this structure was considered to be splenomegaly based on the position and shape.
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Figure 1: Radiograph of the spleen. The abdomen was decreased by lack of fat. In the lateral view, soft tissue density structure (arrows) was identified in the ventral region of the middle abdomen, and this structure was considered to be splenomegaly based on the position and shape.

Mentions: A 2-year-old male Pointer dog was presented to Haemaru Referral Animal Hospital with signs of anorexia, cachexia, and weight loss of a 10-day duration. The body condition score decreased from 3/5 to 1/5 at presentation. The dog was born and raised in Kwangju, Kyonggi-do. It had been used in hunting, and was exposed to ticks. The hunting area covered Bongwha and Youngju, Kyongsangnam-do. Upon physical examination, a mild fever (39.7℃), lethargy, and enlargement of both superficial cervical lymph nodes were noted. Initial screening tests include CBC, serum chemistry, cytology of fine needle aspirates of enlarged lymph nodes, and abdominal radiography. Blood works and serum chemistry results were all within normal limits, except for mild hyperglobulinemia (4.6 g/dl, reference range 2.5-4.5 g/dl) (Table 1). The radiography showed that the spleen was enlarged (Fig. 1). Smears of lymph node aspirates revealed a predominant population of small lymphocytes with a lesser number of medium to large lymphocytes, plasma cells, neutrophils, and macrophages, consistent with neutrophilic lymphadenitis (Fig. 2). Based on this finding, increased serum globulin was considered to be associated with chronic inflammation, and lymphoproliferative neoplasia was ruled out. On the day of the first visit, infectious diseases were screened using a commercial ELISA kit (SNAP 3Dx; IDEXX, USA) for a heartworm (Ehrlichia canis) antigen, and a Lyme antibody (C6 antibody). All the tests were negative except for Lyme disease. On the same day, EDTA whole blood was also submitted to the College of Veterinary Medicine, Chonbuk National University, for PCR testing for E. canis and Babesia (the results were negative for both). To confirm the positive results of the ELISA kit for Lyme disease, the rest of the patient's serum was submitted to an IDEXX Reference Laboratory (USA) for a C6 titer assay and to Antech Diagnostics (USA) for a Lyme IgG assay. The C6 serum titer was 30 U/ml (positive ≥ 30), and the IgG titer was 1 : 256 (negative < 1 : 64). These results were highly suggestive of active borreliosis.


A suspected case of Lyme borreliosis in a hunting dog in Korea.

Choi US, Kim HW, You SE, Youn HJ - J. Vet. Sci. (2009)

Radiograph of the spleen. The abdomen was decreased by lack of fat. In the lateral view, soft tissue density structure (arrows) was identified in the ventral region of the middle abdomen, and this structure was considered to be splenomegaly based on the position and shape.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801107&req=5

Figure 1: Radiograph of the spleen. The abdomen was decreased by lack of fat. In the lateral view, soft tissue density structure (arrows) was identified in the ventral region of the middle abdomen, and this structure was considered to be splenomegaly based on the position and shape.
Mentions: A 2-year-old male Pointer dog was presented to Haemaru Referral Animal Hospital with signs of anorexia, cachexia, and weight loss of a 10-day duration. The body condition score decreased from 3/5 to 1/5 at presentation. The dog was born and raised in Kwangju, Kyonggi-do. It had been used in hunting, and was exposed to ticks. The hunting area covered Bongwha and Youngju, Kyongsangnam-do. Upon physical examination, a mild fever (39.7℃), lethargy, and enlargement of both superficial cervical lymph nodes were noted. Initial screening tests include CBC, serum chemistry, cytology of fine needle aspirates of enlarged lymph nodes, and abdominal radiography. Blood works and serum chemistry results were all within normal limits, except for mild hyperglobulinemia (4.6 g/dl, reference range 2.5-4.5 g/dl) (Table 1). The radiography showed that the spleen was enlarged (Fig. 1). Smears of lymph node aspirates revealed a predominant population of small lymphocytes with a lesser number of medium to large lymphocytes, plasma cells, neutrophils, and macrophages, consistent with neutrophilic lymphadenitis (Fig. 2). Based on this finding, increased serum globulin was considered to be associated with chronic inflammation, and lymphoproliferative neoplasia was ruled out. On the day of the first visit, infectious diseases were screened using a commercial ELISA kit (SNAP 3Dx; IDEXX, USA) for a heartworm (Ehrlichia canis) antigen, and a Lyme antibody (C6 antibody). All the tests were negative except for Lyme disease. On the same day, EDTA whole blood was also submitted to the College of Veterinary Medicine, Chonbuk National University, for PCR testing for E. canis and Babesia (the results were negative for both). To confirm the positive results of the ELISA kit for Lyme disease, the rest of the patient's serum was submitted to an IDEXX Reference Laboratory (USA) for a C6 titer assay and to Antech Diagnostics (USA) for a Lyme IgG assay. The C6 serum titer was 30 U/ml (positive ≥ 30), and the IgG titer was 1 : 256 (negative < 1 : 64). These results were highly suggestive of active borreliosis.

Bottom Line: Spleen was enlarged by radiography, and the lymph nodes showed neutrophilic lymphadenitis by cytological examination.A polymerase chain reaction test for babesiosis and commercial ELISA tests for Ehrlichia canis, heartworm, and Lyme disease was negative except for Lyme disease, which was verified by both an IFA-IgG test and a quantitative C(6) assay.Post-treatment C(6) titer decreased to within normal limits.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Korea.

ABSTRACT
A two-year-old male Pointer had been presented with anorexia, cachexia, and weight loss of 10-day duration. Upon physical examination, fever, lethargy, superficial lymph node enlargement, and tick infestation were noted. The only abnormality in CBC and serum chemistry analyses was mild hyperglobulinemia. Spleen was enlarged by radiography, and the lymph nodes showed neutrophilic lymphadenitis by cytological examination. A polymerase chain reaction test for babesiosis and commercial ELISA tests for Ehrlichia canis, heartworm, and Lyme disease was negative except for Lyme disease, which was verified by both an IFA-IgG test and a quantitative C(6) assay. Doxycycline was administered for 2 weeks and the recovery was uneventful. Post-treatment C(6) titer decreased to within normal limits.

Show MeSH
Related in: MedlinePlus