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Survey of Helicobacter infection in domestic and feral cats in Korea.

Ghil HM, Yoo JH, Jung WS, Chung TH, Youn HY, Hwang CY - J. Vet. Sci. (2009)

Bottom Line: Saliva and feces were evaluated by Helicobacter genus-specific polymerase chain reaction (PCR).Genus-specific PCR positive samples were further evaluated for H. felis and H. pylori using specific primer pairs.H. felis and H. pylori species-specific PCR was uniformly negative.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.

ABSTRACT
Discovery of Helicobacter (H.) pylori has led to a fundamental change in our understanding of gastric diseases in humans. Previous studies have found various Helicobacter spp. in dogs and cats, and pets have been questioned as a zoonotic carrier. The present study surveyed the Helicobacter infections and investigated the presence of H. felis and H. pylori infections in domestic and feral cats in Korea. Sixty-four domestic cats and 101 feral cats were selected from an animal shelter. Saliva and feces were evaluated by Helicobacter genus-specific polymerase chain reaction (PCR). Genus-specific PCR positive samples were further evaluated for H. felis and H. pylori using specific primer pairs. Thirty-six of 64 (56.3%) samples from domestic cats and 92 of 101 (91.1%) samples from feral cats were PCR positive; the positive rate of feces samples was higher than that of saliva samples in both groups. H. felis and H. pylori species-specific PCR was uniformly negative. The prevalence of Helicobacter spp. in feral cats was approximately two-fold higher than that of domestic cats. The fecal-oral route may be more a common transmission route not only between cats but also in humans.

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Related in: MedlinePlus

PCR amplication of Helicobacter (H.) pylori urease B gene fragment. DNA molecular weight standard marker (Lane 1), H. pylori positive control of DNA product at 1,707 bps (Lane 2), negative control (Lane 3), feces of feral cats no.71-73 (Lane 4-6) are shown. All saliva and feces samples of positive Helicobacter genus-specific PCR were H. pylori negative.
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Figure 3: PCR amplication of Helicobacter (H.) pylori urease B gene fragment. DNA molecular weight standard marker (Lane 1), H. pylori positive control of DNA product at 1,707 bps (Lane 2), negative control (Lane 3), feces of feral cats no.71-73 (Lane 4-6) are shown. All saliva and feces samples of positive Helicobacter genus-specific PCR were H. pylori negative.

Mentions: Species-specific PCR was performed on 17 saliva samples and 29 feces samples from the domestic cats and 47 saliva samples and 85 feces samples from the feral cats, which showed a positive result on genus-specific PCR. In H. felis specific PCR, which amplified a 1,200 bp fragment in the positive control (ATCC 49179), none of the samples were positive (Fig. 2). Also, no samples were positive on H. pylori specific PCR, which revealed a 1,700 bp fragment on the positive control (SS1 strain) (Fig. 3).


Survey of Helicobacter infection in domestic and feral cats in Korea.

Ghil HM, Yoo JH, Jung WS, Chung TH, Youn HY, Hwang CY - J. Vet. Sci. (2009)

PCR amplication of Helicobacter (H.) pylori urease B gene fragment. DNA molecular weight standard marker (Lane 1), H. pylori positive control of DNA product at 1,707 bps (Lane 2), negative control (Lane 3), feces of feral cats no.71-73 (Lane 4-6) are shown. All saliva and feces samples of positive Helicobacter genus-specific PCR were H. pylori negative.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801104&req=5

Figure 3: PCR amplication of Helicobacter (H.) pylori urease B gene fragment. DNA molecular weight standard marker (Lane 1), H. pylori positive control of DNA product at 1,707 bps (Lane 2), negative control (Lane 3), feces of feral cats no.71-73 (Lane 4-6) are shown. All saliva and feces samples of positive Helicobacter genus-specific PCR were H. pylori negative.
Mentions: Species-specific PCR was performed on 17 saliva samples and 29 feces samples from the domestic cats and 47 saliva samples and 85 feces samples from the feral cats, which showed a positive result on genus-specific PCR. In H. felis specific PCR, which amplified a 1,200 bp fragment in the positive control (ATCC 49179), none of the samples were positive (Fig. 2). Also, no samples were positive on H. pylori specific PCR, which revealed a 1,700 bp fragment on the positive control (SS1 strain) (Fig. 3).

Bottom Line: Saliva and feces were evaluated by Helicobacter genus-specific polymerase chain reaction (PCR).Genus-specific PCR positive samples were further evaluated for H. felis and H. pylori using specific primer pairs.H. felis and H. pylori species-specific PCR was uniformly negative.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.

ABSTRACT
Discovery of Helicobacter (H.) pylori has led to a fundamental change in our understanding of gastric diseases in humans. Previous studies have found various Helicobacter spp. in dogs and cats, and pets have been questioned as a zoonotic carrier. The present study surveyed the Helicobacter infections and investigated the presence of H. felis and H. pylori infections in domestic and feral cats in Korea. Sixty-four domestic cats and 101 feral cats were selected from an animal shelter. Saliva and feces were evaluated by Helicobacter genus-specific polymerase chain reaction (PCR). Genus-specific PCR positive samples were further evaluated for H. felis and H. pylori using specific primer pairs. Thirty-six of 64 (56.3%) samples from domestic cats and 92 of 101 (91.1%) samples from feral cats were PCR positive; the positive rate of feces samples was higher than that of saliva samples in both groups. H. felis and H. pylori species-specific PCR was uniformly negative. The prevalence of Helicobacter spp. in feral cats was approximately two-fold higher than that of domestic cats. The fecal-oral route may be more a common transmission route not only between cats but also in humans.

Show MeSH
Related in: MedlinePlus