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Composition and morphology of nanocrystals in urines of lithogenic patients and healthy persons.

Gui BS, Xie R, Yao XQ, Li MR, Ouyang JM - Bioinorg Chem Appl (2009)

Bottom Line: TEM results showed that the nanocrystals in healthy urine were dispersive and uniform with a mean size of about 38 nm.In contrast, the nanocrystals in lithogenic urine were much aggregated with a mean size of about 55 nm.The results in this work indicated that the urinary stone formation may be prevented by diminishing the aggregation and the size differentiation of urinary nanocrystals by physical or chemical methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, The Second Hospital of Xi'an Jiaotong University, Xi'an, 710004, China. guibsdoctor@sina.com

ABSTRACT
The composition and morphology of nanocrystals in urines of healthy persons and lithogenic patients were comparatively investigated by means of X-ray diffraction (XRD) and transmission electron microscopy (TEM). It was shown that the main composition of urinary nanocrystals in healthy persons were calcium oxalate dihydrate (COD), uric acid, and ammonium magnesium phosphate (struvite). However, the main compositions of urinary nanocrystals in lithogenic patients were struvite, beta-tricalcium phosphate, uric acid, COD, and calcium oxalate monohydrate (COM). According to the XRD data, the size of nanocrystals was calculated to be 23 approximately 72 nm in healthy urine and 12 approximately 118 nm in lithogenic urine by Scherer formula. TEM results showed that the nanocrystals in healthy urine were dispersive and uniform with a mean size of about 38 nm. In contrast, the nanocrystals in lithogenic urine were much aggregated with a mean size of about 55 nm. The results in this work indicated that the urinary stone formation may be prevented by diminishing the aggregation and the size differentiation of urinary nanocrystals by physical or chemical methods.

No MeSH data available.


XRD patterns of typical urinary crystals of three health persons (H1, H2, H3). (∗: COM, ∗∗: COD, ●: uric acid, ▴: MgNH4PO4 · H2O, ▴ ▴: MgNH4PO4 · 6H2O).
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fig2: XRD patterns of typical urinary crystals of three health persons (H1, H2, H3). (∗: COM, ∗∗: COD, ●: uric acid, ▴: MgNH4PO4 · H2O, ▴ ▴: MgNH4PO4 · 6H2O).

Mentions: XRD was used to investigate the composition of urinary nanocrystals of 16 lithogenic patients and 17 healthy persons. Figures 2 and 3 showed the representative XRD patterns of the urinary nanocrystals in three healthy persons and in three lithogenic patients, respectively. Compared with the standard diagrams (see Table 2) [9], the main peaks in Figure 2(a) were assigned to the (110) crystal plane of MgNH4PO4 · H2O, the (021) plane of uric acid (411), (501), and (730) planes of calcium oxalate dihydrate (COD). The main peaks in Figure 2(b) were assigned to the () plane of uric acid, the (121) plane of MgNH4PO4 · 6H2O, and the (411) and (323) planes of COD crystals. The main peaks in Figure 2(c) were assigned to the (310), (411), and (323) planes of COD crystal. That is, all the three urinary nanocrystals in healthy persons contained COD crystals.


Composition and morphology of nanocrystals in urines of lithogenic patients and healthy persons.

Gui BS, Xie R, Yao XQ, Li MR, Ouyang JM - Bioinorg Chem Appl (2009)

XRD patterns of typical urinary crystals of three health persons (H1, H2, H3). (∗: COM, ∗∗: COD, ●: uric acid, ▴: MgNH4PO4 · H2O, ▴ ▴: MgNH4PO4 · 6H2O).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801016&req=5

fig2: XRD patterns of typical urinary crystals of three health persons (H1, H2, H3). (∗: COM, ∗∗: COD, ●: uric acid, ▴: MgNH4PO4 · H2O, ▴ ▴: MgNH4PO4 · 6H2O).
Mentions: XRD was used to investigate the composition of urinary nanocrystals of 16 lithogenic patients and 17 healthy persons. Figures 2 and 3 showed the representative XRD patterns of the urinary nanocrystals in three healthy persons and in three lithogenic patients, respectively. Compared with the standard diagrams (see Table 2) [9], the main peaks in Figure 2(a) were assigned to the (110) crystal plane of MgNH4PO4 · H2O, the (021) plane of uric acid (411), (501), and (730) planes of calcium oxalate dihydrate (COD). The main peaks in Figure 2(b) were assigned to the () plane of uric acid, the (121) plane of MgNH4PO4 · 6H2O, and the (411) and (323) planes of COD crystals. The main peaks in Figure 2(c) were assigned to the (310), (411), and (323) planes of COD crystal. That is, all the three urinary nanocrystals in healthy persons contained COD crystals.

Bottom Line: TEM results showed that the nanocrystals in healthy urine were dispersive and uniform with a mean size of about 38 nm.In contrast, the nanocrystals in lithogenic urine were much aggregated with a mean size of about 55 nm.The results in this work indicated that the urinary stone formation may be prevented by diminishing the aggregation and the size differentiation of urinary nanocrystals by physical or chemical methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, The Second Hospital of Xi'an Jiaotong University, Xi'an, 710004, China. guibsdoctor@sina.com

ABSTRACT
The composition and morphology of nanocrystals in urines of healthy persons and lithogenic patients were comparatively investigated by means of X-ray diffraction (XRD) and transmission electron microscopy (TEM). It was shown that the main composition of urinary nanocrystals in healthy persons were calcium oxalate dihydrate (COD), uric acid, and ammonium magnesium phosphate (struvite). However, the main compositions of urinary nanocrystals in lithogenic patients were struvite, beta-tricalcium phosphate, uric acid, COD, and calcium oxalate monohydrate (COM). According to the XRD data, the size of nanocrystals was calculated to be 23 approximately 72 nm in healthy urine and 12 approximately 118 nm in lithogenic urine by Scherer formula. TEM results showed that the nanocrystals in healthy urine were dispersive and uniform with a mean size of about 38 nm. In contrast, the nanocrystals in lithogenic urine were much aggregated with a mean size of about 55 nm. The results in this work indicated that the urinary stone formation may be prevented by diminishing the aggregation and the size differentiation of urinary nanocrystals by physical or chemical methods.

No MeSH data available.